Restriction map for
pFN21AE6774
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# NO CUT ENZYMES ( 97 enzymes) #
##################################
AatII Acc65I AclI AcyI AflII
AflIII AgeI AhdI Alw44I ApaLI
AscI AseI AsnI Asp700I Asp718I
AspEI AspI AviII AvrII BanI
BbeI BbrPI BbsI BclI BfrI
BglII BlnI BlpI Bpu1102I BpuAI
BsaAI BsaHI BsiWI BsmBI Bsp1407I
BspLU11I BsrBI BsrGI BssHII Bst1107I
BstAPI BstZ17I CelII CpoI DraI
DrdI Eam1105I EcoNI EcoRI EcoRV
FbaI FspI Hin1I HincII HindII
HindIII HpaI KasI KpnI MfeI
MluI MunI NarI NdeI NheI
NotI NruI PacI PaeR7I PflFI
PinAI PmaCI PmeI PmlI PshBI
Psp1406I PvuI RsrII SalI SbfI
ScaI SexAI SfiI SfoI SgrAI
SmlI SnaBI SpeI SphI Sse8387I
SspBI StuI SwaI Tth111I XcmI
XhoI XmnI
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# ONE CUT ENZYMES ( 50 enzymes) #
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AccI 145 GTAGAC GT^[AC][GT]AC
AccIII 878 TCCGGA T^CCGGA
AocI 381 CCTGAGG CC^T[ACGT]AGG
Aor51HI 296 AGCGCT AGC^GCT
BamHI 249 GGATCC G^GATCC
BbvCI 1154 CCTCAGC CCTCAGC(-5/-2)
BciVI 1279 GGATAC GTATCC(6/5)
BsaBI 421 GATTACTATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
BsaWI 878 TCCGGA [AT]^CCGG[AT]
BseAI 878 TCCGGA T^CCGGA
BseRI 1336 CTCCTC GAGGAG(10/8)
BsgI 1449 CTGCAC GTGCAG(16/14)
BsmI 1223 GAATGC GAATGC(1/-1)
BspDI 1514 ATCGAT AT^CGAT
BspEI 878 TCCGGA T^CCGGA
BsrDI 1003 CATTGC GCAATG(2/0)
BsrFI 1693 GCCGGC [GA]^CCGG[CT]
BssSI 1291 CTCGTG CACGAG(-5/-1)
BstBI 455 TTCGAA TT^CGAA
BstYI 249 GGATCC [GA]^GATC[CT]
Bsu36I 381 CCTGAGG CC^T[ACGT]AGG
Cfr10I 1693 GCCGGC [GA]^CCGG[CT]
ClaI 1514 ATCGAT AT^CGAT
DraIII 1288 CACCTCGTG CAC[ACGT][ACGT][ACGT]^GTG
EarI 858 GAAGAG CTCTTC(1/4)
Eco47III 296 AGCGCT AGC^GCT
Eco81I 381 CCTGAGG CC^T[ACGT]AGG
FseI 1692 GGCCGGCC GGCCGG^CC
HgaI 684 GCGTC GACGC(5/10)
Ksp632I 858 GAAGAG CTCTTC(1/4)
KspI 402 CCGCGG CCGC^GG
MaeII 1475 ACGT A^CGT
MamI 421 GATTACTATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
MflI 249 GGATCC [GA]^GATC[CT]
MroI 878 TCCGGA T^CCGGA
NaeI 1693 GCCGGC GCC^GGC
NgoMIV 1693 GCCGGC G^CCGGC
NspI 1377 GCATGT [GA]CATG^[CT]
NspV 455 TTCGAA TT^CGAA
PflMI 1402 CCACGGCCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
PpuMI 1657 GGGACCC [GA]G^G[AT]CC[CT]
PshAI 1701 GACATTGGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC
SacI 523 GAGCTC GAGCT^C
SacII 402 CCGCGG CCGC^GG
SapI 858 GAAGAGC GCTCTTC(1/4)
SfuI 455 TTCGAA TT^CGAA
SspI 913 AATATT AAT^ATT
Van91I 1402 CCACGGCCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
XbaI 618 TCTAGA T^CTAGA
XhoII 249 GGATCC [GA]^GATC[CT]
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# TWO CUT ENZYMES ( 30 enzymes) #
###################################
AccII 57 CGCG CG^CG
403 CGCG
AcsI 1088 AAATTC [GA]^AATT[CT]
1302 GAATTT
AlwNI 376 CAGAACCTG CAG[ACGT][ACGT][ACGT]^CTG
1570 CAGTACCTG
ApaI 408 GGGCCC GGGCC^C
414 GGGCCC
ApoI 1088 AAATTC [GA]^AATT[CT]
1302 GAATTT
AspHI 195 GAGCAC G[AT]GC[AT]^C
523 GAGCTC
BalI 1229 TGGCCA TGG^CCA
1629 TGGCCA
BcgI 1182 CGATTCCTATGC (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
1241 GCATTGATGTCG
BsaI 132 GAGACC GGTCTC(1/5)
614 GGTCTC
BsiEI 922 CGGCCG CG[GA][CT]^CG
1695 CGGCCG
BsiHKAI 195 GAGCAC G[AT]GC[AT]^C
523 GAGCTC
BspHI 264 TCATGA T^CATGA
959 TCATGA
BspMI 1574 ACCTGC ACCTGC(4/8)
157 GCAGGT
BstEII 303 GGTGACC G^GT[ACGT]ACC
1146 GGTGACC
BstPI 303 GGTGACC G^GT[ACGT]ACC
1146 GGTGACC
BstUI 57 CGCG CG^CG
403 CGCG
EagI 922 CGGCCG C^GGCCG
1695 CGGCCG
EclXI 922 CGGCCG C^GGCCG
1695 CGGCCG
Eco52I 922 CGGCCG C^GGCCG
1695 CGGCCG
EcoO65I 303 GGTGACC G^GT[ACGT]ACC
1146 GGTGACC
EcoT14I 1275 CCATGG C^C[AT][AT]GG
1724 CCATGG
EcoT22I 550 ATGCAT ATGCA^T
805 ATGCAT
MluNI 1229 TGGCCA TGG^CCA
1629 TGGCCA
MscI 1229 TGGCCA TGG^CCA
1629 TGGCCA
MslI 505 CACCCTGATG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
960 CATGAAGGTG
MvnI 57 CGCG CG^CG
403 CGCG
NcoI 1275 CCATGG C^CATGG
1724 CCATGG
NsiI 550 ATGCAT ATGCA^T
805 ATGCAT
RcaI 264 TCATGA T^CATGA
959 TCATGA
StyI 1275 CCATGG C^C[AT][AT]GG
1724 CCATGG
#########################################
# 3 OR MORE CUT ENZYMES ( 81 enzymes) #
#########################################
AciI AfaI AluI AlwI AvaI
AvaII BanII BbvI BcnI BfaI
BglI BmyI BpmI BsaJI BsiYI
BslI BsmAI BsmFI BsoBI Bsp1286I
BsrI BssKI BstF5I BstNI BstXI
Cac8I CfoI Cfr13I DdeI DpnII
DraII DsaI EaeI EcoO109I EcoRII
FauI Fnu4HI FokI HaeII HaeIII
HapII HhaI HinP1I HinfI HpaII
HphI ItaI MaeI MaeIII MboI
MboII MnlI MseI MspA1I MspI
MvaI MwoI NciI NdeII NlaIII
NlaIV PleI PstI PvuII RsaI
Sau3AI Sau96I ScrFI SfaNI SfcI
SmaI TaqI TfiI Tru9I TseI
Tsp45I Tsp509I TspRI TthHB8I XmaCI
XmaI