Restriction map for
pFN21AE3304
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# NO CUT ENZYMES ( 98 enzymes) #
##################################
AatII AccIII AcyI AflII AgeI
AhdI Alw44I AocI Aor51HI ApaI
ApaLI AscI AseI AsnI Asp700I
AspEI AspHI AspI AviII AvrII
BamHI BbeI BbvCI BfrI BlnI
BsaBI BsaHI BseAI BsiEI BsiHKAI
BsiWI BsmBI BspDI BspEI BsrBI
BsrFI BssHII Bst1107I BstAPI BstBI
BstZ17I Bsu36I Cfr10I ClaI CpoI
DraIII EagI Eam1105I EclXI Eco47III
Eco52I Eco81I EcoRI EcoRV FauI
FseI FspI HgaI Hin1I HpaI
KasI KspI MamI MluI MroI
NaeI NarI NdeI NgoMIV NheI
NotI NruI NspV PacI PaeR7I
PflFI PinAI PmeI PshAI PshBI
PvuI RsrII SacI SacII SalI
ScaI SexAI SfiI SfoI SfuI
SgrAI SnaBI SpeI SspI SwaI
Tth111I XhoI XmnI
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# ONE CUT ENZYMES ( 47 enzymes) #
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Acc65I 1048 GGTACC G^GTACC
AccII 1109 CGCG CG^CG
Asp718I 1048 GGTACC G^GTACC
BanI 1048 GGTACC G^G[CT][GA]CC
BanII 688 GAGCCC G[GA]GC[CT]^C
BbsI 76 GTCTTC GAAGAC(2/6)
BciVI 92 GTATCC GTATCC(6/5)
BclI 1661 TGATCA T^GATCA
BlpI 685 GCTGAGC GC^T[ACGT]AGC
BpmI 1115 CTGGAG CTGGAG(16/14)
Bpu1102I 685 GCTGAGC GC^T[ACGT]AGC
BpuAI 76 GTCTTC GAAGAC(2/6)
BsaI 2310 GGTCTC GGTCTC(1/5)
BsaWI 1538 ACCGGA [AT]^CCGG[AT]
BsmI 2163 GAATGC GAATGC(1/-1)
Bsp1407I 67 TGTACA T^GTACA
BsrDI 1493 GCAATG GCAATG(2/0)
BsrGI 67 TGTACA T^GTACA
BssSI 473 CACGAG CACGAG(-5/-1)
BstUI 1109 CGCG CG^CG
CelII 685 GCTGAGC GC^T[ACGT]AGC
DraI 2337 TTTAAA TTT^AAA
DrdI 2302 GACCACGTGGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
EarI 1677 CTCTTC CTCTTC(1/4)
EcoNI 1554 CCTGACCCAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
EcoT22I 2019 ATGCAT ATGCA^T
FbaI 1661 TGATCA T^GATCA
HindIII 844 AAGCTT A^AGCTT
KpnI 1048 GGTACC GGTAC^C
Ksp632I 1677 CTCTTC CTCTTC(1/4)
MfeI 1415 CAATTG C^AATTG
MunI 1415 CAATTG C^AATTG
MvnI 1109 CGCG CG^CG
NcoI 1578 CCATGG C^CATGG
NsiI 2019 ATGCAT ATGCA^T
PpuMI 2006 GGGACCT [GA]G^G[AT]CC[CT]
SapI 1676 GCTCTTC GCTCTTC(1/4)
SmaI 2243 CCCGGG CCC^GGG
SmlI 2260 CTTGAG C^T[CT][GA]AG
SphI 204 GCATGC GCATG^C
SspBI 67 TGTACA T^GTACA
StuI 807 AGGCCT AGG^CCT
TaqI 2230 TCGA T^CGA
TthHB8I 2230 TCGA T^CGA
XbaI 901 TCTAGA T^CTAGA
XmaCI 2243 CCCGGG C^CCGGG
XmaI 2243 CCCGGG C^CCGGG
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# TWO CUT ENZYMES ( 30 enzymes) #
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AccI 1457 GTCTAC GT^[AC][GT]AC
2140 GTAGAC
AclI 1271 AACGTT AA^CGTT
2038 AACGTT
AcsI 1928 AAATTC [GA]^AATT[CT]
1945 AAATTT
AlwNI 540 CAGCATCTG CAG[ACGT][ACGT][ACGT]^CTG
2145 CAGTGCCTG
ApoI 1928 AAATTC [GA]^AATT[CT]
1945 AAATTT
BalI 1130 TGGCCA TGG^CCA
1307 TGGCCA
BbrPI 1670 CACGTG CAC^GTG
2305 CACGTG
BcgI 173 GCAAGTCCTTCG (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
1370 GCACCCGAATCG
BglI 751 GCCCGACTGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
943 GCCCTCTGGGC
BsgI 1526 CTGCAC GTGCAG(16/14)
482 GTGCAG
BspHI 621 TCATGA T^CATGA
2349 TCATGA
BspLU11I 114 ACATGT A^CATGT
1754 ACATGT
BspMI 701 ACCTGC ACCTGC(4/8)
300 GCAGGT
BstXI 1382 CCAAAAACTTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
2064 CCACAAGTATGG
DsaI 239 CCGTGG C^C[GA][CT]GG
1578 CCATGG
EaeI 1130 TGGCCA [CT]^GGCC[GA]
1307 TGGCCA
HaeII 278 GGCGCT [GA]GCGC^[CT]
596 AGCGCC
HincII 655 GTCAAC GT[CT]^[GA]AC
1185 GTTGAC
HindII 655 GTCAAC GT[CT]^[GA]AC
1185 GTTGAC
MluNI 1130 TGGCCA TGG^CCA
1307 TGGCCA
MscI 1130 TGGCCA TGG^CCA
1307 TGGCCA
PflMI 555 CCAGATTTTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
1167 CCATCATCTGG
PmaCI 1670 CACGTG CAC^GTG
2305 CACGTG
PmlI 1670 CACGTG CAC^GTG
2305 CACGTG
Psp1406I 1271 AACGTT AA^CGTT
2038 AACGTT
RcaI 621 TCATGA T^CATGA
2349 TCATGA
SbfI 702 CCTGCAGG CCTGCA^GG
1072 CCTGCAGG
Sse8387I 702 CCTGCAGG CCTGCA^GG
1072 CCTGCAGG
Van91I 555 CCAGATTTTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
1167 CCATCATCTGG
XcmI 209 CCAAGAGAATCGTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
896 CCAAGTCTAGAGTGG
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# 3 OR MORE CUT ENZYMES ( 83 enzymes) #
#########################################
AciI AfaI AflIII AluI AlwI
AvaI AvaII BbvI BcnI BfaI
BglII BmyI BsaAI BsaJI BseRI
BsiYI BslI BsmAI BsmFI BsoBI
Bsp1286I BsrI BssKI BstEII BstF5I
BstNI BstPI BstYI Cac8I CfoI
Cfr13I DdeI DpnII DraII EcoO109I
EcoO65I EcoRII EcoT14I Fnu4HI FokI
HaeIII HapII HhaI HinP1I HinfI
HpaII HphI ItaI MaeI MaeII
MaeIII MboI MboII MflI MnlI
MseI MslI MspA1I MspI MvaI
MwoI NciI NdeII NlaIII NlaIV
NspI PleI PstI PvuII RsaI
Sau3AI Sau96I ScrFI SfaNI SfcI
StyI TfiI Tru9I TseI Tsp45I
Tsp509I TspRI XhoII