Restriction map for
pFN21AE9563
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# NO CUT ENZYMES ( 106 enzymes) #
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AccI AccIII AclI AcsI AflII
AflIII AhdI Alw44I AlwNI Aor51HI
ApaI ApaLI ApoI AscI AseI
AsnI Asp700I AspEI AspHI AviII
AvrII BalI BamHI BbrPI BciVI
BfrI BglII BlnI BlpI Bpu1102I
BsaAI BsaBI BseAI BsiHKAI BsiWI
BsmBI BsmI Bsp1407I BspDI BspEI
BspLU11I BspMI BsrDI BsrGI BssHII
BssSI Bst1107I BstBI BstEII BstPI
BstZ17I CelII ClaI CpoI DraI
DraIII DrdI Eam1105I Eco47III EcoO65I
EcoRI EcoRV FseI FspI KspI
MamI MluI MluNI MroI MscI
NaeI NdeI NgoMIV NheI NruI
NspV PacI PmaCI PmeI PmlI
PshAI PshBI Psp1406I RsrII SacI
SacII SalI SapI SbfI ScaI
SexAI SfiI SfuI SgrAI SmaI
SnaBI SpeI Sse8387I SspBI SspI
StuI SwaI XbaI XmaCI XmaI
XmnI
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# ONE CUT ENZYMES ( 49 enzymes) #
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AatII 484 GACGTC GACGT^C
Acc65I 539 GGTACC G^GTACC
AgeI 1341 ACCGGT A^CCGGT
AocI 1170 CCTGAGG CC^T[ACGT]AGG
Asp718I 539 GGTACC G^GTACC
AvaI 836 CTCGAG C^[CT]CG[GA]G
BbsI 469 GTCTTC GAAGAC(2/6)
BbvCI 1293 GCTGAGG CCTCAGC(-5/-2)
BcgI 907 GCAAACCTTTCG (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
BfaI 1114 CTAG C^TAG
BglI 160 GCCAGCCCGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
BpuAI 469 GTCTTC GAAGAC(2/6)
BsoBI 836 CTCGAG C^[CT]CG[GA]G
BsrBI 48 CCGCTC CCGCTC(-3/-3)
BsrFI 1341 ACCGGT [GA]^CCGG[CT]
BstAPI 1064 GCACTTCCTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
BstYI 443 GGATCT [GA]^GATC[CT]
Bsu36I 1170 CCTGAGG CC^T[ACGT]AGG
Cfr10I 1341 ACCGGT [GA]^CCGG[CT]
DsaI 479 CCATGG C^C[GA][CT]GG
EagI 21 CGGCCG C^GGCCG
EclXI 21 CGGCCG C^GGCCG
Eco52I 21 CGGCCG C^GGCCG
Eco81I 1170 CCTGAGG CC^T[ACGT]AGG
EcoNI 1301 CCTGCACAAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
HincII 10 GTTAAC GT[CT]^[GA]AC
HindII 10 GTTAAC GT[CT]^[GA]AC
HindIII 1258 AAGCTT A^AGCTT
HpaI 10 GTTAAC GTT^AAC
KpnI 539 GGTACC GGTAC^C
MaeI 1114 CTAG C^TAG
MfeI 1380 CAATTG C^AATTG
MflI 443 GGATCT [GA]^GATC[CT]
MunI 1380 CAATTG C^AATTG
NcoI 479 CCATGG C^CATGG
NotI 20 GCGGCCGC GC^GGCCGC
NspI 1241 GCATGC [GA]CATG^[CT]
PaeR7I 836 CTCGAG C^TCGAG
PflMI 649 CCACTCTTTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
PinAI 1341 ACCGGT A^CCGGT
PpuMI 1166 GGGACCT [GA]G^G[AT]CC[CT]
PvuI 284 CGATCG CGAT^CG
SfaNI 1079 GCATC GCATC(5/9)
SphI 1241 GCATGC GCATG^C
Tsp509I 1381 AATT ^AATT
Van91I 649 CCACTCTTTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
XcmI 649 CCACTCTTTGGATGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
XhoI 836 CTCGAG C^TCGAG
XhoII 443 GGATCT [GA]^GATC[CT]
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# TWO CUT ENZYMES ( 25 enzymes) #
###################################
AspI 112 GACCCGGTC GAC[ACGT]^[ACGT][ACGT]GTC
850 GACAGCGTC
BclI 524 TGATCA T^GATCA
695 TGATCA
BsaI 1278 GAGACC GGTCTC(1/5)
1323 GAGACC
BseRI 922 CTCCTC GAGGAG(10/8)
1033 CTCCTC
BsgI 495 CTGCAC GTGCAG(16/14)
1302 CTGCAC
BsrI 1197 CCAGT ACTGG(1/-1)
1203 CCAGT
BstXI 702 CCAGGACTTTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
977 CCACCACCCTGG
EaeI 21 CGGCCG [CT]^GGCC[GA]
374 TGGCCG
EarI 801 GAAGAG CTCTTC(1/4)
898 GAAGAG
EcoT14I 479 CCATGG C^C[AT][AT]GG
543 CCTTGG
EcoT22I 591 ATGCAT ATGCA^T
1243 ATGCAT
FbaI 524 TGATCA T^GATCA
695 TGATCA
Ksp632I 801 GAAGAG CTCTTC(1/4)
898 GAAGAG
MaeII 8 ACGT A^CGT
485 ACGT
MaeIII 154 GTGAC ^GT[ACGT]AC
418 GTCAC
MslI 474 CATCGCCATG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
1103 CATTTCTGTG
NsiI 591 ATGCAT ATGCA^T
1243 ATGCAT
PflFI 112 GACCCGGTC GAC[ACGT]^[ACGT][ACGT]GTC
850 GACAGCGTC
PleI 920 GACTC GAGTC(4/5)
233 GAGTC
PstI 1071 CTGCAG CTGCA^G
1235 CTGCAG
PvuII 1074 CAGCTG CAG^CTG
1232 CAGCTG
StyI 479 CCATGG C^C[AT][AT]GG
543 CCTTGG
TfiI 606 GATTC G^A[AT]TC
1428 GATTC
Tsp45I 154 GTGAC ^GT[GC]AC
418 GTCAC
Tth111I 112 GACCCGGTC GAC[ACGT]^[ACGT][ACGT]GTC
850 GACAGCGTC
#########################################
# 3 OR MORE CUT ENZYMES ( 78 enzymes) #
#########################################
AccII AciI AcyI AfaI AluI
AlwI AvaII BanI BanII BbeI
BbvI BcnI BmyI BpmI BsaHI
BsaJI BsaWI BsiEI BsiYI BslI
BsmAI BsmFI Bsp1286I BspHI BssKI
BstF5I BstNI BstUI Cac8I CfoI
Cfr13I DdeI DpnII DraII EcoO109I
EcoRII FauI Fnu4HI FokI HaeII
HaeIII HapII HgaI HhaI Hin1I
HinP1I HinfI HpaII HphI ItaI
KasI MboI MboII MnlI MseI
MspA1I MspI MvaI MvnI MwoI
NarI NciI NdeII NlaIII NlaIV
RcaI RsaI Sau3AI Sau96I ScrFI
SfcI SfoI SmlI TaqI Tru9I
TseI TspRI TthHB8I