Restriction map for
pFN21AE4377
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# NO CUT ENZYMES ( 142 enzymes) #
##################################
AccII AclI AflII AgeI AhdI
Alw44I AlwNI AocI Aor51HI ApaI
ApaLI AscI AspEI AspI AvaI
AviII AvrII BbeI BbrPI BbvCI
BciVI BclI BcnI BfrI BglI
BglII BlnI BlpI Bpu1102I BsaAI
BsaBI BseRI BsgI BsiEI BsiWI
BsmBI BsoBI Bsp1407I BspDI BspHI
BspMI BsrDI BsrFI BsrGI BssHII
BssSI Bst1107I BstAPI BstBI BstEII
BstPI BstUI BstXI BstZ17I Bsu36I
CelII CfoI Cfr10I ClaI CpoI
DraIII DrdI DsaI EagI Eam1105I
EarI EclXI Eco47III Eco52I Eco81I
EcoNI EcoO65I EcoRV EcoT22I FbaI
FseI FspI HaeII HgaI HhaI
HinP1I HincII HindII HindIII HpaI
KasI Ksp632I KspI MamI MfeI
MluI MspA1I MunI MvnI NaeI
NarI NciI NcoI NdeI NgoMIV
NheI NotI NruI NsiI NspV
PacI PaeR7I PflFI PflMI PinAI
PmaCI PmeI PmlI Psp1406I PvuI
PvuII RcaI RsrII SacII SalI
SapI SbfI ScaI SfiI SfoI
SfuI SgrAI SmaI SnaBI SpeI
SphI Sse8387I SspBI SspI StuI
TaqI Tth111I TthHB8I Van91I XhoI
XmaCI XmaI
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# ONE CUT ENZYMES ( 51 enzymes) #
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AatII 1054 GACGTC GACGT^C
Acc65I 730 GGTACC G^GTACC
AccI 1048 GTCTAC GT^[AC][GT]AC
AccIII 1107 TCCGGA T^CCGGA
AcyI 1054 GACGTC G[GA]^CG[CT]C
AseI 1174 ATTAAT AT^TAAT
AsnI 1174 ATTAAT AT^TAAT
Asp700I 694 GAATTTCTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
Asp718I 730 GGTACC G^GTACC
AspHI 763 GAGCTC G[AT]GC[AT]^C
BamHI 87 GGATCC G^GATCC
BanII 763 GAGCTC G[GA]GC[CT]^C
BbsI 244 GTCTTC GAAGAC(2/6)
BbvI 305 GCAGC GCAGC(8/12)
BcgI 1032 CGACTGGCTTGC (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
BmyI 763 GAGCTC G[AGT]GC[ACT]^C
BpuAI 244 GTCTTC GAAGAC(2/6)
BsaHI 1054 GACGTC G[GA]^CG[CT]C
BsaI 374 GGTCTC GGTCTC(1/5)
BsaWI 1107 TCCGGA [AT]^CCGG[AT]
BseAI 1107 TCCGGA T^CCGGA
BsiHKAI 763 GAGCTC G[AT]GC[AT]^C
BsmFI 230 GTCCC GGGAC(10/14)
Bsp1286I 763 GAGCTC G[AGT]GC[ACT]^C
BspEI 1107 TCCGGA T^CCGGA
BspLU11I 875 ACATGT A^CATGT
BsrBI 1020 GAGCGG CCGCTC(-3/-3)
DraI 813 TTTAAA TTT^AAA
EcoT14I 1150 CCTTGG C^C[AT][AT]GG
FauI 1022 GCGGG CCCGC(4/6)
Fnu4HI 305 GCAGC GC^[ACGT]GC
HapII 1108 CCGG C^CGG
Hin1I 1054 GACGTC G[GA]^CG[CT]C
HpaII 1108 CCGG C^CGG
HphI 1079 TCACC GGTGA(8/7)
ItaI 305 GCAGC GC^[ACGT]GC
KpnI 730 GGTACC GGTAC^C
MroI 1107 TCCGGA T^CCGGA
MspI 1108 CCGG C^CGG
PleI 1071 GACTC GAGTC(4/5)
PshBI 1174 ATTAAT AT^TAAT
PstI 1010 CTGCAG CTGCA^G
SacI 763 GAGCTC GAGCT^C
SexAI 370 ACCTGGT A^CC[AT]GGT
SmlI 521 CTCAAG C^T[CT][GA]AG
StyI 1150 CCTTGG C^C[AT][AT]GG
SwaI 812 ATTTAAAT ATTT^AAAT
TseI 305 GCAGC G^C[AT]GC
XbaI 823 TCTAGA T^CTAGA
XcmI 299 CCAACAGCAGCCTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
XmnI 694 GAATTTCTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
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# TWO CUT ENZYMES ( 24 enzymes) #
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AciI 9 GCGG CCGC(-3/-1)
1022 GCGG
AfaI 626 GTAC GT^AC
731 GTAC
AflIII 341 ACGTGT A^C[GA][CT]GT
875 ACATGT
AvaII 229 GGTCC G^G[AT]CC
601 GGTCC
BalI 489 TGGCCA TGG^CCA
638 TGGCCA
BanI 526 GGCACC G^G[CT][GA]CC
730 GGTACC
BsmI 51 GCATTC GAATGC(1/-1)
1103 GCATTC
BstYI 87 GGATCC [GA]^GATC[CT]
557 AGATCC
EaeI 489 TGGCCA [CT]^GGCC[GA]
638 TGGCCA
EcoRI 194 GAATTC G^AATTC
412 GAATTC
MflI 87 GGATCC [GA]^GATC[CT]
557 AGATCC
MluNI 489 TGGCCA TGG^CCA
638 TGGCCA
MscI 489 TGGCCA TGG^CCA
638 TGGCCA
MslI 102 CATGAATGTG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
996 CATGCTTGTG
MwoI 305 GCAGCCTGGGC GC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GC
610 GCTTCCGTCGC
NspI 875 ACATGT [GA]CATG^[CT]
995 ACATGC
PpuMI 228 AGGTCCC [GA]G^G[AT]CC[CT]
600 GGGTCCT
PshAI 223 GACAAAGGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC
1008 GACTGCAGTC
RsaI 626 GTAC GT^AC
731 GTAC
SfcI 533 CTATAG C^T[GA][CT]AG
1010 CTGCAG
TfiI 134 GATTC G^A[AT]TC
271 GATTC
Tsp45I 286 GTCAC ^GT[GC]AC
1028 GTCAC
TspRI 883 CACTG CA[GC]TG[ACGT][ACGT]^
936 CAGTG
XhoII 87 GGATCC [GA]^GATC[CT]
557 AGATCC
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# 3 OR MORE CUT ENZYMES ( 41 enzymes) #
#########################################
AcsI AluI AlwI ApoI BfaI
BpmI BsaJI BsiYI BslI BsmAI
BsrI BssKI BstF5I BstNI Cac8I
Cfr13I DdeI DpnII DraII EcoO109I
EcoRII FokI HaeIII HinfI MaeI
MaeII MaeIII MboI MboII MnlI
MseI MvaI NdeII NlaIII NlaIV
Sau3AI Sau96I ScrFI SfaNI Tru9I
Tsp509I