Restriction map for
pFN21AE6819
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# NO CUT ENZYMES ( 141 enzymes) #
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Acc65I AccII AccIII AciI AflII
AflIII AgeI AlwI AocI Aor51HI
ApaI AscI AseI AsnI Asp718I
AspI AvaI AviII AvrII BamHI
BanII BbrPI BclI BfaI BfrI
BglI BglII BlnI BlpI Bpu1102I
BsaAI BsaBI BsaI BseAI BsiEI
BsiWI BsoBI Bsp1407I BspDI BspEI
BspHI BspLU11I BsrBI BsrDI BsrFI
BsrGI BssHII Bst1107I BstAPI BstBI
BstEII BstPI BstUI BstXI BstYI
BstZ17I Bsu36I CelII Cfr10I ClaI
CpoI DraII DraIII DrdI DsaI
EagI EarI EclXI Eco47III Eco52I
Eco81I EcoO109I EcoO65I EcoRI EcoRV
EcoT22I FauI FbaI FseI FspI
HgaI HincII HindII HpaI KpnI
Ksp632I KspI MaeI MamI MflI
MluI MroI MslI MvnI NaeI
NcoI NdeI NgoMIV NheI NotI
NruI NsiI NspV PacI PaeR7I
PflFI PinAI PmaCI PmeI PmlI
PpuMI PshAI PshBI PvuI RcaI
RsrII SacI SacII SalI SapI
SbfI ScaI SexAI SfiI SfuI
SgrAI SmaI SnaBI SpeI SphI
Sse8387I SspBI SspI SwaI Tth111I
XbaI XcmI XhoI XhoII XmaCI
XmaI
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# ONE CUT ENZYMES ( 50 enzymes) #
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AatII 69 GACGTC GACGT^C
AccI 376 GTCTAC GT^[AC][GT]AC
AclI 221 AACGTT AA^CGTT
AcsI 329 GAATTT [GA]^AATT[CT]
AhdI 69 GACGTCCGGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
Alw44I 159 GTGCAC G^TGCAC
ApaLI 159 GTGCAC G^TGCAC
ApoI 329 GAATTT [GA]^AATT[CT]
Asp700I 329 GAATTTCTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
AspEI 69 GACGTCCGGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
AvaII 76 GGTCC G^G[AT]CC
BalI 903 TGGCCA TGG^CCA
BbeI 22 GGCGCC GGCGC^C
BbsI 796 GTCTTC GAAGAC(2/6)
BcgI 659 GCATACGCTTCG (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
BciVI 430 GTATCC GTATCC(6/5)
BpmI 553 CTGGAG CTGGAG(16/14)
BpuAI 796 GTCTTC GAAGAC(2/6)
BsaJI 625 CCTTGG C^C[ACGT][ACGT]GG
BsaWI 73 TCCGGT [AT]^CCGG[AT]
BsmBI 669 CGTCTC CGTCTC(1/5)
BsmI 535 GAATGC GAATGC(1/-1)
BssSI 724 CTCGTG CACGAG(-5/-1)
BstNI 901 CCTGG CC^[AT]GG
DraI 1011 TTTAAA TTT^AAA
EaeI 903 TGGCCA [CT]^GGCC[GA]
Eam1105I 69 GACGTCCGGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
EcoNI 274 CCTATTGAAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
EcoRII 901 CCTGG ^CC[AT]GG
EcoT14I 625 CCTTGG C^C[AT][AT]GG
HaeII 22 GGCGCC [GA]GCGC^[CT]
KasI 22 GGCGCC G^GCGCC
MfeI 839 CAATTG C^AATTG
MluNI 903 TGGCCA TGG^CCA
MscI 903 TGGCCA TGG^CCA
MunI 839 CAATTG C^AATTG
MvaI 901 CCTGG CC^[AT]GG
NarI 22 GGCGCC GG^CGCC
NlaIII 938 CATG CATG^
NspI 937 GCATGT [GA]CATG^[CT]
PflMI 801 CCAGCAGCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
Psp1406I 221 AACGTT AA^CGTT
SfoI 22 GGCGCC GGC^GCC
SmlI 323 CTCAAG C^T[CT][GA]AG
StuI 259 AGGCCT AGG^CCT
StyI 625 CCTTGG C^C[AT][AT]GG
TaqI 576 TCGA T^CGA
TthHB8I 576 TCGA T^CGA
Van91I 801 CCAGCAGCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
XmnI 329 GAATTTCTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
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# TWO CUT ENZYMES ( 23 enzymes) #
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AcyI 22 GGCGCC G[GA]^CG[CT]C
69 GACGTC
AspHI 159 GTGCAC G[AT]GC[AT]^C
176 GTGCTC
BanI 22 GGCGCC G^G[CT][GA]CC
318 GGCACC
BbvCI 832 CCTCAGC CCTCAGC(-5/-2)
57 GCTGAGG
BcnI 26 CCGGG CC^[GC]GG
823 CCGGG
BmyI 159 GTGCAC G[AGT]GC[ACT]^C
176 GTGCTC
BsaHI 22 GGCGCC G[GA]^CG[CT]C
69 GACGTC
BsgI 192 CTGCAC GTGCAG(16/14)
368 GTGCAG
BsiHKAI 159 GTGCAC G[AT]GC[AT]^C
176 GTGCTC
Bsp1286I 159 GTGCAC G[AGT]GC[ACT]^C
176 GTGCTC
BspMI 190 ACCTGC ACCTGC(4/8)
6 GCAGGT
Cac8I 136 GCCTGC GC[ACGT]^[ACGT]GC
269 GCTCGC
Hin1I 22 GGCGCC G[GA]^CG[CT]C
69 GACGTC
HindIII 39 AAGCTT A^AGCTT
1007 AAGCTT
MaeII 70 ACGT A^CGT
222 ACGT
MspA1I 164 CAGCTG C[AC]G^C[GT]G
805 CAGCTG
MwoI 57 GCTGAGGAGGC GC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GC
822 GCCGGGTTTGC
NciI 26 CCGGG CC^[GC]GG
823 CCGGG
PstI 138 CTGCAG CTGCA^G
499 CTGCAG
PvuII 164 CAGCTG CAG^CTG
805 CAGCTG
SfaNI 918 GCATC GCATC(5/9)
980 GCATC
Tsp45I 394 GTGAC ^GT[GC]AC
512 GTGAC
TspRI 157 CAGTG CA[GC]TG[ACGT][ACGT]^
738 CACTG
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# 3 OR MORE CUT ENZYMES ( 44 enzymes) #
#########################################
AfaI AluI AlwNI BbvI BseRI
BsiYI BslI BsmAI BsmFI BsrI
BssKI BstF5I CfoI Cfr13I DdeI
DpnII Fnu4HI FokI HaeIII HapII
HhaI HinP1I HinfI HpaII HphI
ItaI MaeIII MboI MboII MnlI
MseI MspI NdeII NlaIV PleI
RsaI Sau3AI Sau96I ScrFI SfcI
TfiI Tru9I TseI Tsp509I