Restriction map for
pFN21AE1982
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# NO CUT ENZYMES ( 113 enzymes) #
##################################
AatII Acc65I AccI AccIII AclI
AflII AflIII AgeI AhdI AscI
Asp718I AspEI AspI AvrII BalI
BamHI BbrPI BbvCI BcgI BclI
BfrI BlnI BlpI Bpu1102I BsaAI
BsaBI BsaI BsaWI BseAI BsiWI
BsmBI BspDI BspEI BspHI BspLU11I
BspMI BsrFI BssHII BssSI Bst1107I
BstBI BstEII BstPI BstZ17I CelII
Cfr10I ClaI CpoI DraI DraIII
DrdI Eam1105I EarI EcoNI EcoO65I
EcoRI EcoT22I FbaI FseI HpaI
KpnI Ksp632I KspI MaeII MamI
MfeI MluI MluNI MroI MscI
MunI NaeI NdeI NgoMIV NotI
NruI NsiI NspI NspV PacI
PaeR7I PflFI PinAI PmaCI PmeI
PmlI PshAI Psp1406I PstI RcaI
RsrII SacI SacII SalI SapI
SbfI ScaI SexAI SfiI SfuI
SgrAI SmlI SnaBI SpeI SphI
Sse8387I SspI StuI SwaI Tth111I
XbaI XcmI XhoI
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# ONE CUT ENZYMES ( 39 enzymes) #
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Alw44I 1516 GTGCAC G^TGCAC
AlwNI 1232 CAGAAGCTG CAG[ACGT][ACGT][ACGT]^CTG
AocI 264 CCTGAGG CC^T[ACGT]AGG
Aor51HI 21 AGCGCT AGC^GCT
ApaLI 1516 GTGCAC G^TGCAC
AseI 745 ATTAAT AT^TAAT
AsnI 745 ATTAAT AT^TAAT
Asp700I 1632 GAAAGTCTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
AviII 1565 TGCGCA TGC^GCA
BciVI 446 GTATCC GTATCC(6/5)
BfaI 625 CTAG C^TAG
BglI 70 GCCGACTCGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
BsgI 515 GTGCAG GTGCAG(16/14)
BsmAI 350 GTCTC GTCTC(1/5)
BsmI 1290 GAATGC GAATGC(1/-1)
Bsp1407I 1646 TGTACA T^GTACA
BsrBI 688 GAGCGG CCGCTC(-3/-3)
BsrDI 1200 CATTGC GCAATG(2/0)
BsrGI 1646 TGTACA T^GTACA
BstXI 233 CCAGCATCGTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
Bsu36I 264 CCTGAGG CC^T[ACGT]AGG
EagI 77 CGGCCG C^GGCCG
EclXI 77 CGGCCG C^GGCCG
Eco47III 21 AGCGCT AGC^GCT
Eco52I 77 CGGCCG C^GGCCG
Eco81I 264 CCTGAGG CC^T[ACGT]AGG
EcoRV 1488 GATATC GAT^ATC
FspI 1565 TGCGCA TGC^GCA
HincII 1161 GTTGAC GT[CT]^[GA]AC
HindII 1161 GTTGAC GT[CT]^[GA]AC
MaeI 625 CTAG C^TAG
NheI 624 GCTAGC G^CTAGC
PflMI 889 CCAGGGTTTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
PshBI 745 ATTAAT AT^TAAT
PvuI 222 CGATCG CGAT^CG
SfcI 156 CTACAG C^T[GA][CT]AG
SspBI 1646 TGTACA T^GTACA
Van91I 889 CCAGGGTTTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
XmnI 1632 GAAAGTCTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
###################################
# TWO CUT ENZYMES ( 25 enzymes) #
###################################
AcsI 843 AAATTT [GA]^AATT[CT]
1429 AAATTT
ApoI 843 AAATTT [GA]^AATT[CT]
1429 AAATTT
AspHI 678 GAGCAC G[AT]GC[AT]^C
1516 GTGCAC
BbeI 149 GGCGCC GGCGC^C
229 GGCGCC
BglII 1244 AGATCT A^GATCT
1278 AGATCT
BseRI 283 GAGGAG GAGGAG(10/8)
1298 GAGGAG
BsiHKAI 678 GAGCAC G[AT]GC[AT]^C
1516 GTGCAC
BsmFI 102 GGGAC GGGAC(10/14)
33 GTCCC
BstAPI 428 GCACGCCCTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
1557 GCAAATTATGC
BstF5I 312 CATCC GGATG(2/0)
363 CATCC
BstYI 1244 AGATCT [GA]^GATC[CT]
1278 AGATCT
DraII 260 AGGACCT [GA]G^G[ACGT]CC[CT]
268 AGGACCC
EcoO109I 260 AGGACCT [GA]G^G[ACGT]CC[CT]
268 AGGACCC
FokI 312 CATCC GGATG(9/13)
363 CATCC
HgaI 800 GACGC GACGC(5/10)
64 GCGTC
HindIII 485 AAGCTT A^AGCTT
857 AAGCTT
KasI 149 GGCGCC G^GCGCC
229 GGCGCC
MflI 1244 AGATCT [GA]^GATC[CT]
1278 AGATCT
NarI 149 GGCGCC GG^CGCC
229 GGCGCC
PpuMI 260 AGGACCT [GA]G^G[AT]CC[CT]
268 AGGACCC
PvuII 1142 CAGCTG CAG^CTG
1607 CAGCTG
SfoI 149 GGCGCC GGC^GCC
229 GGCGCC
TaqI 221 TCGA T^CGA
395 TCGA
TthHB8I 221 TCGA T^CGA
395 TCGA
XhoII 1244 AGATCT [GA]^GATC[CT]
1278 AGATCT
#########################################
# 3 OR MORE CUT ENZYMES ( 81 enzymes) #
#########################################
AccII AciI AcyI AfaI AluI
AlwI ApaI AvaI AvaII BanI
BanII BbsI BbvI BcnI BmyI
BpmI BpuAI BsaHI BsaJI BsiEI
BsiYI BslI BsoBI Bsp1286I BsrI
BssKI BstNI BstUI Cac8I CfoI
Cfr13I DdeI DpnII DsaI EaeI
EcoRII EcoT14I FauI Fnu4HI HaeII
HaeIII HapII HhaI Hin1I HinP1I
HinfI HpaII HphI ItaI MaeIII
MboI MboII MnlI MseI MslI
MspA1I MspI MvaI MvnI MwoI
NciI NcoI NdeII NlaIII NlaIV
PleI RsaI Sau3AI Sau96I ScrFI
SfaNI SmaI StyI TfiI Tru9I
TseI Tsp45I Tsp509I TspRI XmaCI
XmaI