Restriction map for
pFN21AE3093
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# NO CUT ENZYMES ( 134 enzymes) #
##################################
Acc65I AccIII AclI AcsI AfaI
AflII AflIII AgeI AhdI Alw44I
AlwI ApaI ApaLI ApoI AscI
AseI AsnI Asp700I Asp718I AspEI
AspI AviII AvrII BamHI BbrPI
BbsI BbvCI BciVI BclI BfaI
BfrI BglII BlnI BpuAI BsaAI
BsaBI BsaWI BseAI BseRI BsgI
BsiWI BsmBI BsmI Bsp1407I BspDI
BspEI BspHI BspLU11I BsrDI BsrGI
BssHII BssSI Bst1107I BstBI BstXI
BstYI BstZ17I ClaI CpoI DraI
DraII DraIII Eam1105I EcoO109I EcoRI
EcoRV EcoT22I FbaI FseI FspI
HincII HindII HindIII HpaI KpnI
MaeI MamI MfeI MflI MluI
MroI MseI MslI MunI NdeI
NheI NotI NruI NsiI NspI
NspV PacI PaeR7I PflFI PinAI
PmaCI PmeI PmlI PpuMI PshAI
PshBI Psp1406I PvuI RcaI RsaI
RsrII SacI SalI SapI SbfI
ScaI SexAI SfiI SfuI SgrAI
SmaI SmlI SnaBI SpeI SphI
Sse8387I SspBI SspI StuI SwaI
Tru9I Tsp509I Tth111I XbaI XhoI
XhoII XmaCI XmaI XmnI
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# ONE CUT ENZYMES ( 50 enzymes) #
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AatII 724 GACGTC GACGT^C
AccI 733 GTCTAC GT^[AC][GT]AC
AocI 213 CCTGAGG CC^T[ACGT]AGG
Aor51HI 587 AGCGCT AGC^GCT
AvaI 145 CCCGAG C^[CT]CG[GA]G
AvaII 321 GGACC G^G[AT]CC
BalI 281 TGGCCA TGG^CCA
BanII 48 GGGCTC G[GA]GC[CT]^C
BbeI 64 GGCGCC GGCGC^C
BcgI 124 CGAGAGCAGTGC (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
BlpI 50 GCTCAGC GC^T[ACGT]AGC
BpmI 229 CTGGAG CTGGAG(16/14)
Bpu1102I 50 GCTCAGC GC^T[ACGT]AGC
BsaI 493 GAGACC GGTCTC(1/5)
BsiEI 683 CGGCCG CG[GA][CT]^CG
BsmAI 493 GAGAC GTCTC(1/5)
BsoBI 145 CCCGAG C^[CT]CG[GA]G
BsrBI 180 CCGCTC CCGCTC(-3/-3)
BstEII 390 GGTGACC G^GT[ACGT]ACC
BstPI 390 GGTGACC G^GT[ACGT]ACC
Bsu36I 213 CCTGAGG CC^T[ACGT]AGG
CelII 50 GCTCAGC GC^T[ACGT]AGC
DrdI 724 GACGTCAGGGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
EagI 683 CGGCCG C^GGCCG
EarI 85 CTCTTC CTCTTC(1/4)
EclXI 683 CGGCCG C^GGCCG
Eco47III 587 AGCGCT AGC^GCT
Eco52I 683 CGGCCG C^GGCCG
Eco81I 213 CCTGAGG CC^T[ACGT]AGG
EcoNI 664 CCTCCGAAAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
EcoO65I 390 GGTGACC G^GT[ACGT]ACC
HgaI 406 GACGC GACGC(5/10)
KasI 64 GGCGCC G^GCGCC
Ksp632I 85 CTCTTC CTCTTC(1/4)
KspI 374 CCGCGG CCGC^GG
MaeIII 391 GTGAC ^GT[ACGT]AC
MluNI 281 TGGCCA TGG^CCA
MscI 281 TGGCCA TGG^CCA
NarI 64 GGCGCC GG^CGCC
NcoI 109 CCATGG C^CATGG
PflMI 273 CCAGCTCTTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
PstI 595 CTGCAG CTGCA^G
PvuII 598 CAGCTG CAG^CTG
SacII 374 CCGCGG CCGC^GG
SfcI 595 CTGCAG C^T[GA][CT]AG
SfoI 64 GGCGCC GGC^GCC
TfiI 247 GATTC G^A[AT]TC
Tsp45I 391 GTGAC ^GT[GC]AC
Van91I 273 CCAGCTCTTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
XcmI 308 CCAAGAAGGCGCTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
###################################
# TWO CUT ENZYMES ( 22 enzymes) #
###################################
AcyI 64 GGCGCC G[GA]^CG[CT]C
724 GACGTC
AlwNI 130 CAGTGCCTG CAG[ACGT][ACGT][ACGT]^CTG
598 CAGCTGCTG
AspHI 193 GAGCAC G[AT]GC[AT]^C
457 GAGCAC
BsaHI 64 GGCGCC G[GA]^CG[CT]C
724 GACGTC
BsiHKAI 193 GAGCAC G[AT]GC[AT]^C
457 GAGCAC
BspMI 259 ACCTGC ACCTGC(4/8)
394 ACCTGC
BsrI 60 ACTGG ACTGG(1/-1)
79 ACTGG
BstAPI 129 GCAGTGCCTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
597 GCAGCTGCTGC
DpnII 465 GATC ^GATC
783 GATC
Hin1I 64 GGCGCC G[GA]^CG[CT]C
724 GACGTC
HphI 390 GGTGA GGTGA(8/7)
767 TCACC
MboI 465 GATC ^GATC
783 GATC
MboII 86 TCTTC GAAGA(8/7)
171 TCTTC
MspA1I 374 CCGCGG C[AC]G^C[GT]G
598 CAGCTG
NaeI 119 GCCGGC GCC^GGC
526 GCCGGC
NdeII 465 GATC ^GATC
783 GATC
NgoMIV 119 GCCGGC G^CCGGC
526 GCCGGC
NlaIII 110 CATG CATG^
186 CATG
PleI 764 GACTC GAGTC(4/5)
635 GAGTC
Sau3AI 465 GATC ^GATC
783 GATC
TaqI 467 TCGA T^CGA
536 TCGA
TthHB8I 467 TCGA T^CGA
536 TCGA
#########################################
# 3 OR MORE CUT ENZYMES ( 52 enzymes) #
#########################################
AccII AciI AluI BanI BbvI
BcnI BglI BmyI BsaJI BsiYI
BslI BsmFI Bsp1286I BsrFI BssKI
BstF5I BstNI BstUI Cac8I CfoI
Cfr10I Cfr13I DdeI DsaI EaeI
EcoRII EcoT14I FauI Fnu4HI FokI
HaeII HaeIII HapII HhaI HinP1I
HinfI HpaII ItaI MaeII MnlI
MspI MvaI MvnI MwoI NciI
NlaIV Sau96I ScrFI SfaNI StyI
TseI TspRI