Restriction map for
pFN21AE5437
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# NO CUT ENZYMES ( 123 enzymes) #
##################################
AatII Acc65I AccI AccIII AclI
AflIII AgeI AhdI Alw44I AlwNI
AocI Aor51HI ApaI ApaLI AscI
Asp700I Asp718I AspEI AspHI AspI
AviII AvrII BamHI BanII BbrPI
BbsI BbvCI BciVI BclI BglII
BlnI BlpI BpmI Bpu1102I BpuAI
BsaBI BseAI BsgI BsiHKAI BsmBI
BsmI Bsp1407I BspDI BspEI BspLU11I
BsrBI BsrGI BssHII BssSI Bst1107I
BstBI BstXI BstZ17I Bsu36I CelII
ClaI CpoI DraI DraII DrdI
Eam1105I Eco47III Eco81I EcoO109I EcoRI
FbaI FseI FspI HincII HindII
HindIII HpaI KpnI KspI MamI
MluI MroI NdeI NheI NruI
NspI NspV PacI PaeR7I PflFI
PflMI PinAI PmaCI PmeI PmlI
PpuMI Psp1406I PstI PvuI RsrII
SacI SacII SalI SapI SbfI
ScaI SfcI SfiI SfuI SmaI
SnaBI SpeI SphI Sse8387I SspBI
StuI SwaI TaqI TfiI Tth111I
TthHB8I Van91I XbaI XcmI XhoI
XmaCI XmaI XmnI
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# ONE CUT ENZYMES ( 64 enzymes) #
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AccII 400 CGCG CG^CG
AcsI 177 GAATTT [GA]^AATT[CT]
ApoI 177 GAATTT [GA]^AATT[CT]
AseI 457 ATTAAT AT^TAAT
AsnI 457 ATTAAT AT^TAAT
AvaI 44 CTCGGG C^[CT]CG[GA]G
BalI 728 TGGCCA TGG^CCA
BanI 64 GGCGCC G^G[CT][GA]CC
BbeI 64 GGCGCC GGCGC^C
BcgI 13 GCAGGTAGATCG (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
BglI 721 GCCTACATGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
BmyI 208 GTGCCC G[AGT]GC[ACT]^C
BsaAI 475 CACGTA [CT]AC^GT[GA]
BsaWI 143 ACCGGA [AT]^CCGG[AT]
BsiEI 5 CGGCCG CG[GA][CT]^CG
BsiWI 477 CGTACG C^GTACG
BsmFI 779 GGGAC GGGAC(10/14)
BsoBI 44 CTCGGG C^[CT]CG[GA]G
Bsp1286I 208 GTGCCC G[AGT]GC[ACT]^C
BspHI 507 TCATGA T^CATGA
BspMI 13 GCAGGT ACCTGC(4/8)
BsrDI 829 GCAATG GCAATG(2/0)
BstAPI 829 GCAATGTATGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
BstEII 245 GGTTACC G^GT[ACGT]ACC
BstPI 245 GGTTACC G^GT[ACGT]ACC
BstUI 400 CGCG CG^CG
BstYI 624 GGATCT [GA]^GATC[CT]
DraIII 564 CACACTGTG CAC[ACGT][ACGT][ACGT]^GTG
DsaI 824 CCATGG C^C[GA][CT]GG
EagI 5 CGGCCG C^GGCCG
EclXI 5 CGGCCG C^GGCCG
Eco52I 5 CGGCCG C^GGCCG
EcoNI 648 CCTGGAAAAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
EcoO65I 245 GGTTACC G^GT[ACGT]ACC
EcoRV 255 GATATC GAT^ATC
EcoT14I 824 CCATGG C^C[AT][AT]GG
EcoT22I 836 ATGCAT ATGCA^T
HgaI 113 GCGTC GACGC(5/10)
HinfI 316 GACTC G^A[ACGT]TC
KasI 64 GGCGCC G^GCGCC
MfeI 968 CAATTG C^AATTG
MflI 624 GGATCT [GA]^GATC[CT]
MluNI 728 TGGCCA TGG^CCA
MscI 728 TGGCCA TGG^CCA
MspA1I 637 CAGCTG C[AC]G^C[GT]G
MunI 968 CAATTG C^AATTG
MvnI 400 CGCG CG^CG
NarI 64 GGCGCC GG^CGCC
NcoI 824 CCATGG C^CATGG
NotI 4 GCGGCCGC GC^GGCCGC
NsiI 836 ATGCAT ATGCA^T
PleI 316 GACTC GAGTC(4/5)
PshAI 540 GACATTGGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC
PshBI 457 ATTAAT AT^TAAT
PvuII 637 CAGCTG CAG^CTG
RcaI 507 TCATGA T^CATGA
SexAI 760 ACCTGGT A^CC[AT]GGT
SfaNI 964 GATGC GCATC(5/9)
SfoI 64 GGCGCC GGC^GCC
SgrAI 66 CGCCGGCG C[GA]^CCGG[CT]G
SspI 770 AATATT AAT^ATT
StyI 824 CCATGG C^C[AT][AT]GG
Tsp45I 927 GTCAC ^GT[GC]AC
XhoII 624 GGATCT [GA]^GATC[CT]
###################################
# TWO CUT ENZYMES ( 26 enzymes) #
###################################
AcyI 64 GGCGCC G[GA]^CG[CT]C
112 GGCGTC
AflII 501 CTTAAG C^TTAAG
991 CTTAAG
BcnI 275 CCCGG CC^[GC]GG
752 CCCGG
BfaI 619 CTAG C^TAG
940 CTAG
BfrI 501 CTTAAG C^TTAAG
991 CTTAAG
BsaHI 64 GGCGCC G[GA]^CG[CT]C
112 GGCGTC
BsaI 494 GAGACC GGTCTC(1/5)
1004 GAGACC
BsrI 585 CCAGT ACTGG(1/-1)
793 CCAGT
BstF5I 272 CATCC GGATG(2/0)
963 GGATG
CfoI 65 GCGC GCG^C
82 GCGC
EaeI 5 CGGCCG [CT]^GGCC[GA]
728 TGGCCA
EarI 349 GAAGAG CTCTTC(1/4)
687 GAAGAG
FauI 59 GCGGG CCCGC(4/6)
77 GCGGG
FokI 272 CATCC GGATG(9/13)
963 GGATG
HaeII 64 GGCGCC [GA]GCGC^[CT]
81 GGCGCT
HhaI 65 GCGC GCG^C
82 GCGC
Hin1I 64 GGCGCC G[GA]^CG[CT]C
112 GGCGTC
HinP1I 65 GCGC G^CGC
82 GCGC
HphI 1042 GGTGA GGTGA(8/7)
385 TCACC
Ksp632I 349 GAAGAG CTCTTC(1/4)
687 GAAGAG
MaeI 619 CTAG C^TAG
940 CTAG
MaeII 476 ACGT A^CGT
930 ACGT
MboII 349 GAAGA GAAGA(8/7)
687 GAAGA
NaeI 67 GCCGGC GCC^GGC
91 GCCGGC
NciI 275 CCCGG CC^[GC]GG
752 CCCGG
NgoMIV 67 GCCGGC G^CCGGC
91 GCCGGC
#########################################
# 3 OR MORE CUT ENZYMES ( 45 enzymes) #
#########################################
AciI AfaI AluI AlwI AvaII
BbvI BsaJI BseRI BsiYI BslI
BsmAI BsrFI BssKI BstNI Cac8I
Cfr10I Cfr13I DdeI DpnII EcoRII
Fnu4HI HaeIII HapII HpaII ItaI
MaeIII MboI MnlI MseI MslI
MspI MvaI MwoI NdeII NlaIII
NlaIV RsaI Sau3AI Sau96I ScrFI
SmlI Tru9I TseI Tsp509I TspRI