Restriction map for
pFN21AE6379
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# NO CUT ENZYMES ( 176 enzymes) #
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AatII Acc65I AccII AccIII AclI
AcyI AflII AflIII AgeI AhdI
AlwI AlwNI AocI Aor51HI ApaI
AscI AseI AsnI Asp718I AspEI
AspI AvaI AviII AvrII BalI
BamHI BanII BbeI BbrPI BbvCI
BbvI BcgI BciVI BcnI BfrI
BglI BglII BlnI BlpI Bpu1102I
BsaAI BsaHI BsaI BseAI BseRI
BsgI BsiEI BsiWI BsmAI BsmBI
BsmFI BsmI BsoBI Bsp1407I BspEI
BspLU11I BspMI BsrBI BsrDI BsrFI
BsrGI BssHII BssSI Bst1107I BstAPI
BstBI BstEII BstPI BstUI BstYI
BstZ17I Bsu36I CelII CfoI Cfr10I
CpoI DraII DraIII DrdI DsaI
EaeI EagI Eam1105I EclXI Eco47III
Eco52I Eco81I EcoNI EcoO109I EcoO65I
EcoRI EcoRV EcoT22I FauI Fnu4HI
FseI FspI HaeII HaeIII HgaI
HhaI Hin1I HinP1I HindIII ItaI
KasI KpnI KspI MaeII MfeI
MflI MluI MluNI MroI MscI
MslI MunI MvnI MwoI NaeI
NarI NciI NcoI NgoMIV NheI
NotI NruI NsiI NspI NspV
PacI PaeR7I PflFI PflMI PinAI
PmaCI PmeI PmlI PpuMI PshAI
PshBI Psp1406I PstI PvuI RsrII
SacI SacII SalI SapI SbfI
ScaI SexAI SfcI SfiI SfoI
SfuI SgrAI SmaI SnaBI SpeI
SphI Sse8387I SspBI SspI StuI
SwaI TseI TspRI Tth111I Van91I
XbaI XcmI XhoI XhoII XmaCI
XmaI
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# ONE CUT ENZYMES ( 45 enzymes) #
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AccI 518 GTCTAC GT^[AC][GT]AC
AciI 378 CCGC CCGC(-3/-1)
Alw44I 713 GTGCAC G^TGCAC
ApaLI 713 GTGCAC G^TGCAC
Asp700I 730 GAATCAATTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
AspHI 713 GTGCAC G[AT]GC[AT]^C
BanI 367 GGTGCC G^G[CT][GA]CC
BclI 632 TGATCA T^GATCA
BmyI 713 GTGCAC G[AGT]GC[ACT]^C
BsaBI 935 GATTTGAATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
BsaJI 195 CCAAGG C^C[ACGT][ACGT]GG
BsaWI 848 ACCGGA [AT]^CCGG[AT]
BsiHKAI 713 GTGCAC G[AT]GC[AT]^C
BsiYI 718 CCAGTAAAAGG CC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GG
BslI 718 CCAGTAAAAGG CC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GG
Bsp1286I 713 GTGCAC G[AGT]GC[ACT]^C
BspDI 40 ATCGAT AT^CGAT
BspHI 255 TCATGA T^CATGA
BstXI 68 CCAGGAAAATGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
Cac8I 459 GCTTGC GC[ACGT]^[ACGT]GC
ClaI 40 ATCGAT AT^CGAT
DraI 558 TTTAAA TTT^AAA
EarI 121 CTCTTC CTCTTC(1/4)
EcoT14I 195 CCAAGG C^C[AT][AT]GG
FbaI 632 TGATCA T^GATCA
HapII 849 CCGG C^CGG
HincII 478 GTTAAC GT[CT]^[GA]AC
HindII 478 GTTAAC GT[CT]^[GA]AC
HpaI 478 GTTAAC GTT^AAC
HpaII 849 CCGG C^CGG
Ksp632I 121 CTCTTC CTCTTC(1/4)
MamI 935 GATTTGAATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
MspA1I 628 CAGCTG C[AC]G^C[GT]G
MspI 849 CCGG C^CGG
NlaIII 256 CATG CATG^
PleI 303 GACTC GAGTC(4/5)
PvuII 628 CAGCTG CAG^CTG
RcaI 255 TCATGA T^CATGA
SfaNI 958 GCATC GCATC(5/9)
SmlI 381 CTTGAG C^T[CT][GA]AG
StyI 195 CCAAGG C^C[AT][AT]GG
TaqI 41 TCGA T^CGA
Tsp45I 400 GTGAC ^GT[GC]AC
TthHB8I 41 TCGA T^CGA
XmnI 730 GAATCAATTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
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# TWO CUT ENZYMES ( 13 enzymes) #
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AvaII 25 GGTCC G^G[AT]CC
658 GGACC
BbsI 413 GAAGAC GAAGAC(2/6)
844 GAAGAC
BpmI 944 CTCCAG CTGGAG(16/14)
293 CTGGAG
BpuAI 413 GAAGAC GAAGAC(2/6)
844 GAAGAC
BsrI 662 CCAGT ACTGG(1/-1)
718 CCAGT
BstF5I 564 GGATG GGATG(2/0)
708 GGATG
Cfr13I 25 GGTCC G^G[ACGT]CC
658 GGACC
DdeI 305 CTCAG C^T[ACGT]AG
904 CTGAG
FokI 564 GGATG GGATG(9/13)
708 GGATG
MaeIII 400 GTGAC ^GT[ACGT]AC
624 GTTAC
NdeI 350 CATATG CA^TATG
616 CATATG
NlaIV 367 GGTGCC GG[ACGT]^[ACGT]CC
658 GGACCC
Sau96I 25 GGTCC G^G[ACGT]CC
658 GGACC
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# 3 OR MORE CUT ENZYMES ( 24 enzymes) #
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AcsI AfaI AluI ApoI BfaI
BssKI BstNI DpnII EcoRII HinfI
HphI MaeI MboI MboII MnlI
MseI MvaI NdeII RsaI Sau3AI
ScrFI TfiI Tru9I Tsp509I