Restriction map for
pFN21AE2187
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# NO CUT ENZYMES ( 127 enzymes) #
##################################
AatII Acc65I AccIII AclI AcyI
AfaI AflII AflIII AgeI AhdI
Alw44I AocI Aor51HI ApaI ApaLI
AscI AseI AsnI Asp700I Asp718I
AspEI AspI AvrII BalI BamHI
BanI BbeI BciVI BclI BfrI
BglI BglII BlnI BsaBI BsaHI
BsaWI BseAI BsiWI Bsp1407I BspDI
BspEI BspHI BspLU11I BsrBI BsrDI
BsrFI BsrGI BssHII BssSI Bst1107I
BstAPI BstBI BstEII BstPI BstYI
BstZ17I Bsu36I Cfr10I ClaI CpoI
DraII DraIII Eam1105I Eco47III Eco81I
EcoNI EcoO109I EcoO65I EcoT22I FbaI
FseI Hin1I HpaI KasI KpnI
KspI MamI MfeI MflI MluI
MluNI MroI MscI MslI MspA1I
MunI NaeI NarI NgoMIV NheI
NotI NruI NsiI NspI NspV
PacI PaeR7I PflFI PflMI PinAI
PmeI PpuMI PshBI Psp1406I PvuI
PvuII RcaI RsaI RsrII SacII
SapI ScaI SfiI SfoI SfuI
SgrAI SnaBI SphI SspBI SspI
SwaI Tth111I Van91I XbaI XhoI
XhoII XmnI
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# ONE CUT ENZYMES ( 54 enzymes) #
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AccII 274 CGCG CG^CG
AlwI 1059 GATCC GGATC(4/5)
AlwNI 865 CAGTATCTG CAG[ACGT][ACGT][ACGT]^CTG
AvaII 1356 GGTCC G^G[AT]CC
AviII 281 TGCGCA TGC^GCA
BbrPI 1195 CACGTG CAC^GTG
BbsI 1190 GTCTTC GAAGAC(2/6)
BbvCI 1361 CCTCAGC CCTCAGC(-5/-2)
BcgI 1366 GCAGCCACCTCG (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
BlpI 313 GCTGAGC GC^T[ACGT]AGC
Bpu1102I 313 GCTGAGC GC^T[ACGT]AGC
BpuAI 1190 GTCTTC GAAGAC(2/6)
BsaAI 1195 CACGTG [CT]AC^GT[GA]
BsaI 232 GAGACC GGTCTC(1/5)
BsiEI 59 CGGCCG CG[GA][CT]^CG
BsmFI 1357 GTCCC GGGAC(10/14)
BsmI 846 GCATTC GAATGC(1/-1)
BspMI 278 ACCTGC ACCTGC(4/8)
BstUI 274 CGCG CG^CG
BstXI 179 CCAACCGCCTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
CelII 313 GCTGAGC GC^T[ACGT]AGC
DpnII 1059 GATC ^GATC
DraI 792 TTTAAA TTT^AAA
DrdI 706 GACCAGCCCGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
EaeI 59 CGGCCG [CT]^GGCC[GA]
EagI 59 CGGCCG C^GGCCG
EclXI 59 CGGCCG C^GGCCG
Eco52I 59 CGGCCG C^GGCCG
EcoRV 739 GATATC GAT^ATC
FspI 281 TGCGCA TGC^GCA
HaeII 10 AGCGCC [GA]GCGC^[CT]
HapII 1186 CCGG C^CGG
HgaI 777 GCGTC GACGC(5/10)
HindIII 667 AAGCTT A^AGCTT
HpaII 1186 CCGG C^CGG
MboI 1059 GATC ^GATC
MspI 1186 CCGG C^CGG
MvnI 274 CGCG CG^CG
NdeI 1112 CATATG CA^TATG
NdeII 1059 GATC ^GATC
PleI 976 GAGTC GAGTC(4/5)
PmaCI 1195 CACGTG CAC^GTG
PmlI 1195 CACGTG CAC^GTG
PshAI 1305 GACCCCAGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC
Sau3AI 1059 GATC ^GATC
SbfI 690 CCTGCAGG CCTGCA^GG
SexAI 1101 ACCTGGT A^CC[AT]GGT
SmaI 1185 CCCGGG CCC^GGG
SmlI 903 CTTGAG C^T[CT][GA]AG
SpeI 636 ACTAGT A^CTAGT
Sse8387I 690 CCTGCAGG CCTGCA^GG
XcmI 980 CCAGCTTAGAAATGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
XmaCI 1185 CCCGGG C^CCGGG
XmaI 1185 CCCGGG C^CCGGG
###################################
# TWO CUT ENZYMES ( 32 enzymes) #
###################################
AccI 84 GTCGAC GT^[AC][GT]AC
956 GTCGAC
AspHI 172 GAGCTC G[AT]GC[AT]^C
430 GAGCTC
AvaI 90 CTCGGG C^[CT]CG[GA]G
1185 CCCGGG
BanII 172 GAGCTC G[GA]GC[CT]^C
430 GAGCTC
BcnI 1185 CCCGG CC^[GC]GG
1186 CCGGG
BmyI 172 GAGCTC G[AGT]GC[ACT]^C
430 GAGCTC
BsgI 1022 CTGCAC GTGCAG(16/14)
1198 GTGCAG
BsiHKAI 172 GAGCTC G[AT]GC[AT]^C
430 GAGCTC
BsiYI 162 CCGCCTGGAGG CC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GG
303 CCAAGCCAGGG
BslI 162 CCGCCTGGAGG CC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GG
303 CCAAGCCAGGG
BsmBI 714 CGTCTC CGTCTC(1/5)
585 GAGACG
BsoBI 90 CTCGGG C^[CT]CG[GA]G
1185 CCCGGG
Bsp1286I 172 GAGCTC G[AGT]GC[ACT]^C
430 GAGCTC
BsrI 540 ACTGG ACTGG(1/-1)
1309 CCAGT
Cfr13I 1274 GGCCC G^G[ACGT]CC
1356 GGTCC
EarI 895 GAAGAG CTCTTC(1/4)
941 GAAGAG
EcoRI 328 GAATTC G^AATTC
415 GAATTC
EcoT14I 746 CCATGG C^C[AT][AT]GG
1218 CCATGG
FauI 37 GCGGG CCCGC(4/6)
110 GCGGG
Ksp632I 895 GAAGAG CTCTTC(1/4)
941 GAAGAG
MaeII 882 ACGT A^CGT
1196 ACGT
NciI 1185 CCCGG CC^[GC]GG
1186 CCGGG
NcoI 746 CCATGG C^CATGG
1218 CCATGG
NlaIV 908 GGCTCC GG[ACGT]^[ACGT]CC
1356 GGTCCC
SacI 172 GAGCTC GAGCT^C
430 GAGCTC
SalI 84 GTCGAC G^TCGAC
956 GTCGAC
Sau96I 1274 GGCCC G^G[ACGT]CC
1356 GGTCC
StuI 1090 AGGCCT AGG^CCT
1231 AGGCCT
StyI 746 CCATGG C^C[AT][AT]GG
1218 CCATGG
TaqI 85 TCGA T^CGA
957 TCGA
TfiI 686 GAATC G^A[AT]TC
757 GATTC
TthHB8I 85 TCGA T^CGA
957 TCGA
#########################################
# 3 OR MORE CUT ENZYMES ( 45 enzymes) #
#########################################
AciI AcsI AluI ApoI BbvI
BfaI BpmI BsaJI BseRI BsmAI
BssKI BstF5I BstNI Cac8I CfoI
DdeI DsaI EcoRII Fnu4HI FokI
HaeIII HhaI HinP1I HincII HindII
HinfI HphI ItaI MaeI MaeIII
MboII MnlI MseI MvaI MwoI
NlaIII PstI ScrFI SfaNI SfcI
Tru9I TseI Tsp45I Tsp509I TspRI