Restriction map for
pFN21AE2771
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# NO CUT ENZYMES ( 165 enzymes) #
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AatII Acc65I AccI AccII AccIII
AciI AclI AcsI AcyI AflII
AflIII AgeI AhdI Alw44I AocI
ApaI ApaLI ApoI AscI Asp700I
Asp718I AspEI AspHI AspI AvaI
AviII BalI BanI BanII BbeI
BbrPI BbvCI BcgI BclI BcnI
BfrI BglI BlpI BmyI Bpu1102I
BsaBI BsaHI BsaWI BseAI BseRI
BsgI BsiHKAI BsiWI BsmBI BsmFI
BsmI BsoBI Bsp1286I BspDI BspEI
BspHI BspLU11I BspMI BsrBI BsrFI
BssHII BssSI Bst1107I BstBI BstEII
BstPI BstUI BstZ17I Bsu36I CelII
Cfr10I ClaI CpoI DraII DraIII
DrdI DsaI EaeI EagI Eam1105I
EclXI Eco52I Eco81I EcoNI EcoO109I
EcoO65I EcoRI EcoRV EcoT22I FauI
FbaI FseI FspI HapII Hin1I
HincII HindII HindIII HpaI HpaII
KasI KpnI KspI MamI MfeI
MluI MluNI MroI MscI MspA1I
MspI MunI MvnI NaeI NarI
NciI NcoI NgoMIV NheI NotI
NruI NsiI NspV PacI PaeR7I
PflFI PflMI PinAI PmaCI PmeI
PmlI PpuMI PshAI Psp1406I PvuII
RcaI RsrII SacI SacII SalI
SapI SbfI ScaI SexAI SfiI
SfoI SfuI SgrAI SmaI SmlI
SnaBI SpeI SphI Sse8387I SspI
SwaI TaqI Tth111I TthHB8I Van91I
XbaI XhoI XmaCI XmaI XmnI
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# ONE CUT ENZYMES ( 27 enzymes) #
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AlwNI 207 CAGTCTCTG CAG[ACGT][ACGT][ACGT]^CTG
Aor51HI 414 AGCGCT AGC^GCT
BbsI 1260 GAAGAC GAAGAC(2/6)
BglII 615 AGATCT A^GATCT
BpuAI 1260 GAAGAC GAAGAC(2/6)
BsaAI 381 TACGTG [CT]AC^GT[GA]
BsaI 564 GAGACC GGTCTC(1/5)
BsiEI 399 CGATCG CG[GA][CT]^CG
BsrDI 930 CATTGC GCAATG(2/0)
BstAPI 471 GCAGAGGATGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
BstXI 1287 CCATGCCTATGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
CfoI 415 GCGC GCG^C
DdeI 65 CTCAG C^T[ACGT]AG
EarI 1015 GAAGAG CTCTTC(1/4)
Eco47III 414 AGCGCT AGC^GCT
HaeII 414 AGCGCT [GA]GCGC^[CT]
HgaI 1409 GCGTC GACGC(5/10)
HhaI 415 GCGC GCG^C
HinP1I 415 GCGC G^CGC
Ksp632I 1015 GAAGAG CTCTTC(1/4)
MslI 1288 CATGCCTATG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
NdeI 1247 CATATG CA^TATG
NspI 1462 ACATGC [GA]CATG^[CT]
PleI 101 GAGTC GAGTC(4/5)
PvuI 399 CGATCG CGAT^CG
TfiI 657 GAATC G^A[AT]TC
XcmI 1256 CCACGAAGACTGTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
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# TWO CUT ENZYMES ( 24 enzymes) #
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AseI 1105 ATTAAT AT^TAAT
1302 ATTAAT
AsnI 1105 ATTAAT AT^TAAT
1302 ATTAAT
AvrII 1212 CCTAGG C^CTAGG
1332 CCTAGG
BciVI 1082 GGATAC GTATCC(6/5)
1458 GGATAC
BlnI 1212 CCTAGG C^CTAGG
1332 CCTAGG
BpmI 608 CTCCAG CTGGAG(16/14)
269 CTGGAG
BsiYI 19 CCTGACGGTGG CC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GG
1342 CCCATCGTTGG
BslI 19 CCTGACGGTGG CC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GG
1342 CCCATCGTTGG
Bsp1407I 107 TGTACA T^GTACA
1505 TGTACA
BsrGI 107 TGTACA T^GTACA
1505 TGTACA
BssKI 155 CCTGG ^CC[ACGT]GG
168 CCTGG
BstNI 155 CCTGG CC^[AT]GG
168 CCTGG
DraI 534 TTTAAA TTT^AAA
901 TTTAAA
EcoRII 155 CCTGG ^CC[AT]GG
168 CCTGG
EcoT14I 1212 CCTAGG C^C[AT][AT]GG
1332 CCTAGG
HinfI 101 GAGTC G^A[ACGT]TC
657 GAATC
MvaI 155 CCTGG CC^[AT]GG
168 CCTGG
MwoI 471 GCAGAGGATGC GC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GC
1153 GCCTTGTGTGC
PshBI 1105 ATTAAT AT^TAAT
1302 ATTAAT
PstI 204 CTGCAG CTGCA^G
469 CTGCAG
ScrFI 155 CCTGG CC^[ACGT]GG
168 CCTGG
SspBI 107 TGTACA T^GTACA
1505 TGTACA
StuI 273 AGGCCT AGG^CCT
408 AGGCCT
StyI 1212 CCTAGG C^C[AT][AT]GG
1332 CCTAGG
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# 3 OR MORE CUT ENZYMES ( 42 enzymes) #
#########################################
AfaI AluI AlwI AvaII BamHI
BbvI BfaI BsaJI BsmAI BsrI
BstF5I BstYI Cac8I Cfr13I DpnII
Fnu4HI FokI HaeIII HphI ItaI
MaeI MaeII MaeIII MboI MboII
MflI MnlI MseI NdeII NlaIII
NlaIV RsaI Sau3AI Sau96I SfaNI
SfcI Tru9I TseI Tsp45I Tsp509I
TspRI XhoII