Restriction map for
pFN21AB6261
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# NO CUT ENZYMES ( 124 enzymes) #
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AatII AccI AccIII AcyI AflIII
AgeI Alw44I Aor51HI ApaI ApaLI
AscI AseI AsnI Asp700I AvaI
AviII AvrII BalI BamHI BbeI
BbrPI BciVI BclI BglI BglII
BlnI BlpI Bpu1102I BsaAI BsaBI
BsaHI BsaWI BseAI BsgI BsiWI
BsoBI BspDI BspEI BspHI BspLU11I
BsrFI BssHII Bst1107I BstBI BstEII
BstPI BstXI BstZ17I CelII Cfr10I
ClaI CpoI DrdI EaeI EagI
EclXI Eco47III Eco52I EcoO65I EcoRI
EcoRV EcoT22I FbaI FseI FspI
HaeII HgaI Hin1I HincII HindII
HpaI KasI KspI MamI MfeI
MluI MluNI MroI MscI MslI
MunI NaeI NarI NgoMIV NheI
NotI NruI NsiI NspV PaeR7I
PflMI PinAI PmaCI PmeI PmlI
PshAI PshBI PvuI PvuII RcaI
RsrII SacII SalI SapI SbfI
ScaI SexAI SfiI SfoI SfuI
SgrAI SmaI SnaBI SpeI SphI
Sse8387I StuI SwaI Van91I XbaI
XhoI XmaCI XmaI XmnI
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# ONE CUT ENZYMES ( 39 enzymes) #
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Acc65I 1933 GGTACC G^GTACC
AclI 1200 AACGTT AA^CGTT
AflII 1923 CTTAAG C^TTAAG
AocI 2002 CCTGAGG CC^T[ACGT]AGG
Asp718I 1933 GGTACC G^GTACC
AspI 874 GACCCAGTC GAC[ACGT]^[ACGT][ACGT]GTC
BcgI 194 CGAGCACCTTGC (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
BfrI 1923 CTTAAG C^TTAAG
BsaI 760 GAGACC GGTCTC(1/5)
BseRI 46 GAGGAG GAGGAG(10/8)
BsiEI 581 CGACCG CG[GA][CT]^CG
BsmBI 446 GAGACG CGTCTC(1/5)
BsmI 1470 GCATTC GAATGC(1/-1)
Bsp1407I 1316 TGTACA T^GTACA
BspMI 979 ACCTGC ACCTGC(4/8)
BsrDI 1207 GCAATG GCAATG(2/0)
BsrGI 1316 TGTACA T^GTACA
BssSI 851 CTCGTG CACGAG(-5/-1)
BstAPI 1275 GCAGATCATGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
Bsu36I 2002 CCTGAGG CC^T[ACGT]AGG
DraII 1493 GGGACCT [GA]G^G[ACGT]CC[CT]
DsaI 521 CCATGG C^C[GA][CT]GG
Eco81I 2002 CCTGAGG CC^T[ACGT]AGG
EcoNI 1714 CCTTTCTCAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
EcoO109I 1493 GGGACCT [GA]G^G[ACGT]CC[CT]
KpnI 1933 GGTACC GGTAC^C
MspA1I 58 CAGCGG C[AC]G^C[GT]G
NcoI 521 CCATGG C^CATGG
NdeI 1049 CATATG CA^TATG
NspI 1441 GCATGT [GA]CATG^[CT]
PacI 1813 TTAATTAA TTAAT^TAA
PflFI 874 GACCCAGTC GAC[ACGT]^[ACGT][ACGT]GTC
PpuMI 1493 GGGACCT [GA]G^G[AT]CC[CT]
Psp1406I 1200 AACGTT AA^CGTT
SacI 1378 GAGCTC GAGCT^C
SspBI 1316 TGTACA T^GTACA
SspI 1340 AATATT AAT^ATT
Tth111I 874 GACCCAGTC GAC[ACGT]^[ACGT][ACGT]GTC
XcmI 765 CCACTATGAAAATGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
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# TWO CUT ENZYMES ( 24 enzymes) #
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AhdI 448 GACGTGGGGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
1663 GACTACTGGTC
AlwNI 118 CAGAAGCTG CAG[ACGT][ACGT][ACGT]^CTG
968 CAGAAGCTG
AspEI 448 GACGTGGGGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
1663 GACTACTGGTC
BanI 4 GGCACC G^G[CT][GA]CC
1933 GGTACC
BbsI 856 GAAGAC GAAGAC(2/6)
871 GAAGAC
BbvCI 732 CCTCAGC CCTCAGC(-5/-2)
51 GCTGAGG
BcnI 405 CCCGG CC^[GC]GG
572 CCCGG
BfaI 1369 CTAG C^TAG
1519 CTAG
BpuAI 856 GAAGAC GAAGAC(2/6)
871 GAAGAC
BsrBI 147 CCGCTC CCGCTC(-3/-3)
1263 CCGCTC
DraI 690 TTTAAA TTT^AAA
1006 TTTAAA
DraIII 863 CACCGAGTG CAC[ACGT][ACGT][ACGT]^GTG
1231 CACATTGTG
Eam1105I 448 GACGTGGGGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
1663 GACTACTGGTC
EarI 724 GAAGAG CTCTTC(1/4)
757 GAAGAG
FauI 12 GCGGG CCCGC(4/6)
60 GCGGG
HapII 406 CCGG C^CGG
573 CCGG
HpaII 406 CCGG C^CGG
573 CCGG
Ksp632I 724 GAAGAG CTCTTC(1/4)
757 GAAGAG
MaeI 1369 CTAG C^TAG
1519 CTAG
MspI 406 CCGG C^CGG
573 CCGG
NciI 405 CCCGG CC^[GC]GG
572 CCCGG
PstI 124 CTGCAG CTGCA^G
965 CTGCAG
SfcI 124 CTGCAG C^T[GA][CT]AG
965 CTGCAG
TspRI 458 CACTG CA[GC]TG[ACGT][ACGT]^
1673 CACTG
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# 3 OR MORE CUT ENZYMES ( 71 enzymes) #
#########################################
AccII AciI AcsI AfaI AluI
AlwI ApoI AspHI AvaII BanII
BbvI BmyI BpmI BsaJI BsiHKAI
BsiYI BslI BsmAI BsmFI Bsp1286I
BsrI BssKI BstF5I BstNI BstUI
BstYI Cac8I CfoI Cfr13I DdeI
DpnII EcoRII EcoT14I Fnu4HI FokI
HaeIII HhaI HinP1I HindIII HinfI
HphI ItaI MaeII MaeIII MboI
MboII MflI MnlI MseI MvaI
MvnI MwoI NdeII NlaIII NlaIV
PleI RsaI Sau3AI Sau96I ScrFI
SfaNI SmlI StyI TaqI TfiI
Tru9I TseI Tsp45I Tsp509I TthHB8I
XhoII