Restriction map for
pFN21AE6496
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# NO CUT ENZYMES ( 121 enzymes) #
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AatII Acc65I AccIII AclI AcsI
AflII AgeI AhdI AlwI AocI
Aor51HI ApoI AscI AseI AsnI
Asp700I Asp718I AspEI AspI AvaII
AviII AvrII BamHI BbvCI BcgI
BciVI BclI BfrI BglII BlnI
BpmI BsaBI BsaWI BseAI BsiWI
BsmBI BsmI BspDI BspEI BspHI
BspLU11I Bst1107I BstAPI BstBI BstEII
BstPI BstXI BstYI BstZ17I Bsu36I
ClaI CpoI DraI DrdI EagI
Eam1105I EarI EclXI Eco47III Eco52I
Eco81I EcoNI EcoO65I EcoRI EcoRV
EcoT22I FbaI FseI FspI HindIII
HpaI KpnI Ksp632I MamI MfeI
MflI MluI MroI MseI MunI
NcoI NdeI NotI NruI NsiI
NspV PacI PaeR7I PflFI PflMI
PinAI PmeI PpuMI PshAI PshBI
Psp1406I PvuI RcaI RsrII SacI
SapI ScaI SexAI SfiI SfuI
SgrAI SnaBI SpeI SphI SspI
StuI SwaI Tru9I Tsp45I Tsp509I
Tth111I Van91I XbaI XhoI XhoII
XmnI
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# ONE CUT ENZYMES ( 30 enzymes) #
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AflIII 578 ACACGT A^C[GA][CT]GT
BbrPI 939 CACGTG CAC^GTG
BbsI 358 GAAGAC GAAGAC(2/6)
BpuAI 358 GAAGAC GAAGAC(2/6)
BsaAI 939 CACGTG [CT]AC^GT[GA]
BsaI 1152 GGTCTC GGTCTC(1/5)
BseRI 616 GAGGAG GAGGAG(10/8)
BsgI 806 CTGCAC GTGCAG(16/14)
BsiEI 438 CGACCG CG[GA][CT]^CG
Bsp1407I 146 TGTACA T^GTACA
BsrGI 146 TGTACA T^GTACA
BssSI 1110 CTCGTG CACGAG(-5/-1)
DraIII 1321 CACATTGTG CAC[ACGT][ACGT][ACGT]^GTG
HincII 1189 GTCGAC GT[CT]^[GA]AC
HindII 1189 GTCGAC GT[CT]^[GA]AC
KspI 8 CCGCGG CCGC^GG
NheI 741 GCTAGC G^CTAGC
PleI 219 GACTC GAGTC(4/5)
PmaCI 939 CACGTG CAC^GTG
PmlI 939 CACGTG CAC^GTG
SacII 8 CCGCGG CCGC^GG
SalI 1189 GTCGAC G^TCGAC
SbfI 549 CCTGCAGG CCTGCA^GG
SmaI 29 CCCGGG CCC^GGG
SmlI 420 CTTGAG C^T[CT][GA]AG
Sse8387I 549 CCTGCAGG CCTGCA^GG
SspBI 146 TGTACA T^GTACA
TfiI 4 GAATC G^A[AT]TC
XmaCI 29 CCCGGG C^CCGGG
XmaI 29 CCCGGG C^CCGGG
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# TWO CUT ENZYMES ( 24 enzymes) #
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Alw44I 635 GTGCAC G^TGCAC
1327 GTGCAC
AlwNI 553 CAGGGCCTG CAG[ACGT][ACGT][ACGT]^CTG
1066 CAGTGGCTG
ApaLI 635 GTGCAC G^TGCAC
1327 GTGCAC
AspHI 635 GTGCAC G[AT]GC[AT]^C
1327 GTGCAC
BanI 95 GGCGCC G^G[CT][GA]CC
1102 GGCGCC
BbeI 95 GGCGCC GGCGC^C
1102 GGCGCC
BsiHKAI 635 GTGCAC G[AT]GC[AT]^C
1327 GTGCAC
BsmAI 1153 GTCTC GTCTC(1/5)
1240 GTCTC
BsmFI 1365 GGGAC GGGAC(10/14)
297 GTCCC
BsrBI 1131 CCGCTC CCGCTC(-3/-3)
423 GAGCGG
BsrDI 870 CATTGC GCAATG(2/0)
933 CATTGC
DsaI 8 CCGCGG C^C[GA][CT]GG
875 CCGTGG
EcoT14I 389 CCAAGG C^C[AT][AT]GG
1356 CCTTGG
HinfI 4 GAATC G^A[ACGT]TC
219 GACTC
HphI 893 TCACC GGTGA(8/7)
1031 TCACC
KasI 95 GGCGCC G^GCGCC
1102 GGCGCC
MaeIII 129 GTTAC ^GT[ACGT]AC
902 GTAAC
MboII 358 GAAGA GAAGA(8/7)
366 GAAGA
NarI 95 GGCGCC GG^CGCC
1102 GGCGCC
NspI 140 ACATGC [GA]CATG^[CT]
188 ACATGC
PvuII 305 CAGCTG CAG^CTG
403 CAGCTG
SfoI 95 GGCGCC GGC^GCC
1102 GGCGCC
StyI 389 CCAAGG C^C[AT][AT]GG
1356 CCTTGG
XcmI 821 CCAACCTGCCAGTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
1057 CCACACCTGCAGTGG
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# 3 OR MORE CUT ENZYMES ( 83 enzymes) #
#########################################
AccI AccII AciI AcyI AfaI
AluI ApaI AvaI BalI BanII
BbvI BcnI BfaI BglI BlpI
BmyI Bpu1102I BsaHI BsaJI BsiYI
BslI BsoBI Bsp1286I BspMI BsrFI
BsrI BssHII BssKI BstF5I BstNI
BstUI Cac8I CelII CfoI Cfr10I
Cfr13I DdeI DpnII DraII EaeI
EcoO109I EcoRII FauI Fnu4HI FokI
HaeII HaeIII HapII HgaI HhaI
Hin1I HinP1I HpaII ItaI MaeI
MaeII MboI MluNI MnlI MscI
MslI MspA1I MspI MvaI MvnI
MwoI NaeI NciI NdeII NgoMIV
NlaIII NlaIV PstI RsaI Sau3AI
Sau96I ScrFI SfaNI SfcI TaqI
TseI TspRI TthHB8I