Restriction map for
pFN21AE2299
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# NO CUT ENZYMES ( 119 enzymes) #
##################################
AatII Acc65I AccII AccIII AclI
AcsI AflII AgeI Alw44I AocI
ApaI ApaLI ApoI AscI AseI
AsnI Asp700I Asp718I AspI AvaI
BamHI BbeI BcgI BciVI BfrI
BglI BglII BsaBI BseAI BsiEI
BsiWI BsmBI BsmI BsoBI BspDI
BspEI BspLU11I BsrBI BsrFI BssHII
Bst1107I BstBI BstEII BstPI BstUI
BstYI BstZ17I Bsu36I Cfr10I ClaI
DraI DraIII DsaI EagI EclXI
Eco52I Eco81I EcoO65I EcoRI EcoRV
EcoT22I FseI HindIII HpaI KasI
KpnI KspI MamI MfeI MflI
MluI MroI MunI MvnI NaeI
NarI NcoI NdeI NgoMIV NheI
NotI NruI NsiI NspI NspV
PacI PaeR7I PflFI PflMI PinAI
PmeI PshBI Psp1406I PvuI SacII
SbfI ScaI SexAI SfiI SfoI
SfuI SgrAI SmaI SnaBI SpeI
SphI Sse8387I SspI SwaI TfiI
Tsp509I Tth111I Van91I XbaI XhoI
XhoII XmaCI XmaI XmnI
###################################
# ONE CUT ENZYMES ( 58 enzymes) #
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AcyI 1104 GACGCC G[GA]^CG[CT]C
AflIII 287 ACACGT A^C[GA][CT]GT
AhdI 452 GACTCTATGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
AlwI 1031 GGATC GGATC(4/5)
Aor51HI 635 AGCGCT AGC^GCT
AspEI 452 GACTCTATGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
AviII 200 TGCGCA TGC^GCA
AvrII 418 CCTAGG C^CTAGG
BalI 915 TGGCCA TGG^CCA
BbsI 766 GTCTTC GAAGAC(2/6)
BbvCI 813 GCTGAGG CCTCAGC(-5/-2)
BclI 824 TGATCA T^GATCA
BcnI 962 CCGGG CC^[GC]GG
BfaI 419 CTAG C^TAG
BlnI 418 CCTAGG C^CTAGG
BlpI 442 GCTGAGC GC^T[ACGT]AGC
Bpu1102I 442 GCTGAGC GC^T[ACGT]AGC
BpuAI 766 GTCTTC GAAGAC(2/6)
BsaHI 1104 GACGCC G[GA]^CG[CT]C
BsaWI 644 TCCGGT [AT]^CCGG[AT]
BseRI 901 GAGGAG GAGGAG(10/8)
Bsp1407I 275 TGTACA T^GTACA
BspHI 489 TCATGA T^CATGA
BsrDI 1035 CATTGC GCAATG(2/0)
BsrGI 275 TGTACA T^GTACA
BsrI 120 ACTGG ACTGG(1/-1)
BssSI 898 CACGAG CACGAG(-5/-1)
CelII 442 GCTGAGC GC^T[ACGT]AGC
CpoI 646 CGGTCCG CG^G[AT]CCG
DraII 221 GGGTCCC [GA]G^G[ACGT]CC[CT]
DrdI 721 GACATCAGTGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
EaeI 915 TGGCCA [CT]^GGCC[GA]
Eam1105I 452 GACTCTATGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
EarI 400 CTCTTC CTCTTC(1/4)
Eco47III 635 AGCGCT AGC^GCT
EcoNI 1209 CCTCTACAAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
EcoO109I 221 GGGTCCC [GA]G^G[ACGT]CC[CT]
FbaI 824 TGATCA T^GATCA
FspI 200 TGCGCA TGC^GCA
Hin1I 1104 GACGCC G[GA]^CG[CT]C
Ksp632I 400 CTCTTC CTCTTC(1/4)
MaeI 419 CTAG C^TAG
MluNI 915 TGGCCA TGG^CCA
MscI 915 TGGCCA TGG^CCA
MseI 209 TTAA T^TAA
MspA1I 1026 CAGCTG C[AC]G^C[GT]G
NciI 962 CCGGG CC^[GC]GG
PpuMI 221 GGGTCCC [GA]G^G[AT]CC[CT]
PshAI 86 GACGAGGGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC
PstI 73 CTGCAG CTGCA^G
PvuII 1026 CAGCTG CAG^CTG
RcaI 489 TCATGA T^CATGA
RsrII 646 CGGTCCG CG^G[AT]CCG
SacI 445 GAGCTC GAGCT^C
SapI 399 GCTCTTC GCTCTTC(1/4)
SspBI 275 TGTACA T^GTACA
StuI 1169 AGGCCT AGG^CCT
Tru9I 209 TTAA T^TAA
###################################
# TWO CUT ENZYMES ( 24 enzymes) #
###################################
AccI 300 GTCGAC GT^[AC][GT]AC
933 GTCGAC
AlwNI 972 CAGCCACTG CAG[ACGT][ACGT][ACGT]^CTG
1129 CAGACCCTG
BanI 126 GGTGCC G^G[CT][GA]CC
1230 GGTGCC
BanII 445 GAGCTC G[GA]GC[CT]^C
1194 GAGCCC
BbrPI 475 CACGTG CAC^GTG
919 CACGTG
BsaAI 475 CACGTG [CT]AC^GT[GA]
919 CACGTG
BsgI 1126 GTGCAG GTGCAG(16/14)
1281 GTGCAG
BspMI 749 ACCTGC ACCTGC(4/8)
883 GCAGGT
BstAPI 390 GCACATCTTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
1166 GCAAGGCCTGC
BstXI 780 CCACAGCAGTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
1154 CCACAAAGGTGG
FauI 928 GCGGG CCCGC(4/6)
996 GCGGG
HapII 645 CCGG C^CGG
962 CCGG
HgaI 1104 GACGC GACGC(5/10)
313 GCGTC
HincII 300 GTCGAC GT[CT]^[GA]AC
933 GTCGAC
HindII 300 GTCGAC GT[CT]^[GA]AC
933 GTCGAC
HinfI 452 GACTC G^A[ACGT]TC
652 GACTC
HpaII 645 CCGG C^CGG
962 CCGG
HphI 886 GGTGA GGTGA(8/7)
434 TCACC
MspI 645 CCGG C^CGG
962 CCGG
PleI 452 GACTC GAGTC(4/5)
652 GACTC
PmaCI 475 CACGTG CAC^GTG
919 CACGTG
PmlI 475 CACGTG CAC^GTG
919 CACGTG
SalI 300 GTCGAC G^TCGAC
933 GTCGAC
XcmI 70 CCACTGCAGAGCTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
563 CCAAGACCTTCCTGG
#########################################
# 3 OR MORE CUT ENZYMES ( 57 enzymes) #
#########################################
AciI AfaI AluI AspHI AvaII
BbvI BmyI BpmI BsaI BsaJI
BsiHKAI BsiYI BslI BsmAI BsmFI
Bsp1286I BssKI BstF5I BstNI Cac8I
CfoI Cfr13I DdeI DpnII EcoRII
EcoT14I Fnu4HI FokI HaeII HaeIII
HhaI HinP1I ItaI MaeII MaeIII
MboI MboII MnlI MslI MvaI
MwoI NdeII NlaIII NlaIV RsaI
Sau3AI Sau96I ScrFI SfaNI SfcI
SmlI StyI TaqI TseI Tsp45I
TspRI TthHB8I