Restriction map for
pFN21AE2077
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# NO CUT ENZYMES ( 147 enzymes) #
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Acc65I AccIII AflII AflIII AgeI
AhdI Alw44I AlwI AocI ApaI
ApaLI AscI AseI AsnI Asp718I
AspEI AspHI AspI AvaI AviII
AvrII BalI BamHI BanI BanII
BbeI BbrPI BbvCI BcgI BciVI
BcnI BfrI BglI BglII BlnI
BlpI BmyI BpmI Bpu1102I BsaAI
BsaBI BsaI BseAI BseRI BsiEI
BsiHKAI BsiWI BsmBI BsmFI BsoBI
Bsp1286I BspDI BspEI BspLU11I BspMI
BsrBI BsrDI BsrFI BssHII BstAPI
BstBI BstEII BstPI BstYI Bsu36I
CelII Cfr10I ClaI CpoI DraIII
EaeI EagI Eam1105I EarI EclXI
Eco52I Eco81I EcoO65I EcoRI EcoT22I
FauI FseI FspI HpaI KasI
KpnI Ksp632I KspI MamI MfeI
MflI MluI MluNI MroI MscI
MspA1I MunI MwoI NaeI NarI
NciI NgoMIV NotI NruI NsiI
NspV PacI PaeR7I PflFI PflMI
PinAI PmaCI PmeI PmlI PpuMI
PshAI PshBI PstI PvuI PvuII
RsrII SacI SacII SalI SapI
SbfI SexAI SfiI SfoI SfuI
SgrAI SmaI SmlI SnaBI SpeI
SphI Sse8387I SspI StuI SwaI
Tth111I Van91I XbaI XhoI XhoII
XmaCI XmaI
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# ONE CUT ENZYMES ( 37 enzymes) #
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AatII 1285 GACGTC GACGT^C
AccI 1428 GTATAC GT^[AC][GT]AC
AccII 2232 CGCG CG^CG
AciI 361 GCGG CCGC(-3/-1)
AclI 1322 AACGTT AA^CGTT
AlwNI 937 CAGGAGCTG CAG[ACGT][ACGT][ACGT]^CTG
Aor51HI 1447 AGCGCT AGC^GCT
BsaWI 2024 ACCGGA [AT]^CCGG[AT]
BsmAI 624 GTCTC GTCTC(1/5)
BssSI 1245 CACGAG CACGAG(-5/-1)
Bst1107I 1428 GTATAC GTA^TAC
BstUI 2232 CGCG CG^CG
BstXI 1343 CCATGTCTATGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
BstZ17I 1428 GTATAC GTA^TAC
CfoI 1448 GCGC GCG^C
DraI 1951 TTTAAA TTT^AAA
DrdI 1279 GACAAGGACGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
Eco47III 1447 AGCGCT AGC^GCT
EcoNI 987 CCTTACTCAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
EcoRV 121 GATATC GAT^ATC
HaeII 1447 AGCGCT [GA]GCGC^[CT]
HapII 2025 CCGG C^CGG
HgaI 2206 GCGTC GACGC(5/10)
HhaI 1448 GCGC GCG^C
HinP1I 1448 GCGC G^CGC
HincII 797 GTCAAC GT[CT]^[GA]AC
HindII 797 GTCAAC GT[CT]^[GA]AC
HindIII 665 AAGCTT A^AGCTT
HpaII 2025 CCGG C^CGG
MspI 2025 CCGG C^CGG
MvnI 2232 CGCG CG^CG
NheI 423 GCTAGC G^CTAGC
NlaIV 1563 GGCTCC GG[ACGT]^[ACGT]CC
Psp1406I 1322 AACGTT AA^CGTT
SfaNI 1897 GATGC GCATC(5/9)
Tsp45I 1184 GTGAC ^GT[GC]AC
XcmI 1635 CCAACATTTCAGTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
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# TWO CUT ENZYMES ( 27 enzymes) #
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AcyI 1285 GACGTC G[GA]^CG[CT]C
2205 GGCGTC
AvaII 363 GGACC G^G[AT]CC
2022 GGACC
BbsI 520 GAAGAC GAAGAC(2/6)
1234 GAAGAC
BclI 1080 TGATCA T^GATCA
1370 TGATCA
BpuAI 520 GAAGAC GAAGAC(2/6)
1234 GAAGAC
BsaHI 1285 GACGTC G[GA]^CG[CT]C
2205 GGCGTC
BsgI 290 GTGCAG GTGCAG(16/14)
2009 GTGCAG
BsmI 482 GAATGC GAATGC(1/-1)
1310 GAATGC
Bsp1407I 1608 TGTACA T^GTACA
1678 TGTACA
BspHI 789 TCATGA T^CATGA
2060 TCATGA
BsrGI 1608 TGTACA T^GTACA
1678 TGTACA
BssKI 1590 CCAGG ^CC[ACGT]GG
2202 CCAGG
BstNI 1590 CCAGG CC^[AT]GG
2202 CCAGG
DraII 965 AGGGCCT [GA]G^G[ACGT]CC[CT]
1109 AGGCCCT
EcoO109I 965 AGGGCCT [GA]G^G[ACGT]CC[CT]
1109 AGGCCCT
EcoRII 1590 CCAGG ^CC[AT]GG
2202 CCAGG
FbaI 1080 TGATCA T^GATCA
1370 TGATCA
Hin1I 1285 GACGTC G[GA]^CG[CT]C
2205 GGCGTC
MvaI 1590 CCAGG CC^[AT]GG
2202 CCAGG
NcoI 543 CCATGG C^CATGG
1724 CCATGG
NdeI 928 CATATG CA^TATG
1715 CATATG
NspI 834 ACATGC [GA]CATG^[CT]
1001 ACATGC
RcaI 789 TCATGA T^CATGA
2060 TCATGA
ScaI 246 AGTACT AGT^ACT
1178 AGTACT
ScrFI 1590 CCAGG CC^[ACGT]GG
2202 CCAGG
SfcI 1534 CTACAG C^T[GA][CT]AG
2048 CTATAG
SspBI 1608 TGTACA T^GTACA
1678 TGTACA
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# 3 OR MORE CUT ENZYMES ( 47 enzymes) #
#########################################
AcsI AfaI AluI ApoI Asp700I
BbvI BfaI BsaJI BsiYI BslI
BsrI BstF5I Cac8I Cfr13I DdeI
DpnII DsaI EcoT14I Fnu4HI FokI
HaeIII HinfI HphI ItaI MaeI
MaeII MaeIII MboI MboII MnlI
MseI MslI NdeII NlaIII PleI
RsaI Sau3AI Sau96I StyI TaqI
TfiI Tru9I TseI Tsp509I TspRI
TthHB8I XmnI