Restriction map for
pFN21AE5781
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# NO CUT ENZYMES ( 108 enzymes) #
##################################
AatII AclI AflII AflIII AgeI
AhdI Alw44I AocI ApaI ApaLI
AscI AseI AsnI Asp700I AspEI
AspI AviII AvrII BamHI BbrPI
BbvCI BcgI BfrI BglI BglII
BlnI BlpI Bpu1102I BsaI BsiWI
BsmBI Bsp1407I BspDI BspLU11I BspMI
BsrDI BsrGI BssHII BssSI BstAPI
BstBI Bsu36I CelII ClaI CpoI
DraI DrdI Eam1105I EarI Eco81I
EcoRI EcoRV EcoT22I FseI FspI
HgaI HincII HindII HpaI Ksp632I
KspI MfeI MluI MseI MunI
NdeI NheI NotI NruI NsiI
NspI NspV PacI PaeR7I PflFI
PflMI PinAI PmaCI PmeI PmlI
PshAI PshBI Psp1406I PvuI RsrII
SacI SacII SalI SapI SbfI
ScaI SfiI SfuI SgrAI SmlI
SnaBI SpeI SphI Sse8387I SspBI
StuI SwaI Tru9I Tth111I Van91I
XbaI XhoI XmnI
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# ONE CUT ENZYMES ( 60 enzymes) #
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Acc65I 110 GGTACC G^GTACC
AccIII 180 TCCGGA T^CCGGA
AcyI 1027 GGCGCC G[GA]^CG[CT]C
Aor51HI 911 AGCGCT AGC^GCT
Asp718I 110 GGTACC G^GTACC
AspHI 31 GTGCTC G[AT]GC[AT]^C
BalI 1880 TGGCCA TGG^CCA
BanII 1723 GGGCTC G[GA]GC[CT]^C
BbeI 1027 GGCGCC GGCGC^C
BciVI 974 GGATAC GTATCC(6/5)
BsaAI 256 TACGTG [CT]AC^GT[GA]
BsaBI 710 GATCCTCATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
BsaHI 1027 GGCGCC G[GA]^CG[CT]C
BsaWI 180 TCCGGA [AT]^CCGG[AT]
BseAI 180 TCCGGA T^CCGGA
BsgI 392 GTGCAG GTGCAG(16/14)
BsiHKAI 31 GTGCTC G[AT]GC[AT]^C
BspEI 180 TCCGGA T^CCGGA
BspHI 1532 TCATGA T^CATGA
BsrBI 16 CCGCTC CCGCTC(-3/-3)
Bst1107I 1261 GTATAC GTA^TAC
BstEII 1392 GGTAACC G^GT[ACGT]ACC
BstPI 1392 GGTAACC G^GT[ACGT]ACC
BstXI 1871 CCACTGTGCTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
BstYI 1688 GGATCT [GA]^GATC[CT]
BstZ17I 1261 GTATAC GTA^TAC
DraII 429 AGGTCCT [GA]G^G[ACGT]CC[CT]
EagI 53 CGGCCG C^GGCCG
EclXI 53 CGGCCG C^GGCCG
Eco47III 911 AGCGCT AGC^GCT
Eco52I 53 CGGCCG C^GGCCG
EcoNI 1464 CCTGGGGGAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
EcoO109I 429 AGGTCCT [GA]G^G[ACGT]CC[CT]
EcoO65I 1392 GGTAACC G^GT[ACGT]ACC
Hin1I 1027 GGCGCC G[GA]^CG[CT]C
HindIII 570 AAGCTT A^AGCTT
KasI 1027 GGCGCC G^GCGCC
KpnI 110 GGTACC GGTAC^C
MamI 710 GATCCTCATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
MflI 1688 GGATCT [GA]^GATC[CT]
MluNI 1880 TGGCCA TGG^CCA
MroI 180 TCCGGA T^CCGGA
MscI 1880 TGGCCA TGG^CCA
MspA1I 1766 CAGCTG C[AC]G^C[GT]G
NaeI 55 GCCGGC GCC^GGC
NarI 1027 GGCGCC GG^CGCC
NcoI 731 CCATGG C^CATGG
NgoMIV 55 GCCGGC G^CCGGC
PpuMI 429 AGGTCCT [GA]G^G[AT]CC[CT]
PstI 905 CTGCAG CTGCA^G
PvuII 1766 CAGCTG CAG^CTG
RcaI 1532 TCATGA T^CATGA
SexAI 696 ACCAGGT A^CC[AT]GGT
SfoI 1027 GGCGCC GGC^GCC
SmaI 106 CCCGGG CCC^GGG
SspI 1055 AATATT AAT^ATT
Tsp45I 1006 GTCAC ^GT[GC]AC
XhoII 1688 GGATCT [GA]^GATC[CT]
XmaCI 106 CCCGGG C^CCGGG
XmaI 106 CCCGGG C^CCGGG
###################################
# TWO CUT ENZYMES ( 25 enzymes) #
###################################
AccI 982 GTAGAC GT^[AC][GT]AC
1261 GTATAC
AccII 753 CGCG CG^CG
1676 CGCG
AfaI 111 GTAC GT^AC
1554 GTAC
AlwNI 237 CAGAGACTG CAG[ACGT][ACGT][ACGT]^CTG
1362 CAGAAACTG
AvaI 106 CCCGGG C^[CT]CG[GA]G
1696 CCCGAG
BclI 203 TGATCA T^GATCA
1583 TGATCA
BfaI 461 CTAG C^TAG
631 CTAG
BpmI 599 CTCCAG CTGGAG(16/14)
580 CTGGAG
BsiEI 53 CGGCCG CG[GA][CT]^CG
624 CGACCG
BsmI 514 GAATGC GAATGC(1/-1)
722 GCATTC
BsoBI 106 CCCGGG C^[CT]CG[GA]G
1696 CCCGAG
BsrFI 55 GCCGGC [GA]^CCGG[CT]
626 ACCGGC
BstUI 753 CGCG CG^CG
1676 CGCG
Cfr10I 55 GCCGGC [GA]^CCGG[CT]
626 ACCGGC
DraIII 386 CACATGGTG CAC[ACGT][ACGT][ACGT]^GTG
458 CACCTAGTG
DsaI 731 CCATGG C^C[GA][CT]GG
1895 CCACGG
EaeI 53 CGGCCG [CT]^GGCC[GA]
1880 TGGCCA
EcoT14I 731 CCATGG C^C[AT][AT]GG
1353 CCAAGG
FauI 917 CCCGC CCCGC(4/6)
1423 CCCGC
FbaI 203 TGATCA T^GATCA
1583 TGATCA
MaeI 461 CTAG C^TAG
631 CTAG
MvnI 753 CGCG CG^CG
1676 CGCG
RsaI 111 GTAC GT^AC
1554 GTAC
SfaNI 1371 GATGC GCATC(5/9)
1490 GCATC
StyI 731 CCATGG C^C[AT][AT]GG
1353 CCAAGG
#########################################
# 3 OR MORE CUT ENZYMES ( 65 enzymes) #
#########################################
AciI AcsI AluI AlwI ApoI
AvaII BanI BbsI BbvI BcnI
BmyI BpuAI BsaJI BseRI BsiYI
BslI BsmAI BsmFI Bsp1286I BsrI
BssKI BstF5I BstNI Cac8I CfoI
Cfr13I DdeI DpnII EcoRII Fnu4HI
FokI HaeII HaeIII HapII HhaI
HinP1I HinfI HpaII HphI ItaI
MaeII MaeIII MboI MboII MnlI
MslI MspI MvaI MwoI NciI
NdeII NlaIII NlaIV PleI Sau3AI
Sau96I ScrFI SfcI TaqI TfiI
TseI Tsp509I TspRI TthHB8I XcmI