################################## # NO CUT ENZYMES ( 93 enzymes) # ################################## AatII Acc65I AccI AccIII AcyI AgeI AhdI ApaI AscI Asp718I AspEI AvrII BamHI BbeI BbrPI BcgI BglI BlnI BsaBI BsaHI BsaI BseAI BsiWI Bsp1407I BspDI BspEI BsrBI BsrDI BsrFI BsrGI BssHII Bst1107I BstBI BstEII BstPI BstXI BstZ17I Cfr10I ClaI CpoI DraI DraIII DrdI EagI Eam1105I EclXI Eco52I EcoNI EcoO65I EcoRI EcoRV FauI FseI Hin1I KasI KpnI MamI MluI MroI NaeI NarI NgoMIV NheI NotI NruI NspV PacI PaeR7I PinAI PmaCI PmeI PmlI PshAI PvuI RsrII SalI SapI SbfI ScaI SexAI SfiI SfoI SfuI SgrAI SnaBI SphI Sse8387I SspBI StuI SwaI XbaI XcmI XhoI ################################### # ONE CUT ENZYMES ( 54 enzymes) # ################################### AclI 1817 AACGTT AA^CGTT AflII 1618 CTTAAG C^TTAAG AflIII 570 ACATGT A^C[GA][CT]GT AlwNI 2413 CAGGACCTG CAG[ACGT][ACGT][ACGT]^CTG AocI 1729 CCTGAGG CC^T[ACGT]AGG Aor51HI 2473 AGCGCT AGC^GCT AseI 628 ATTAAT AT^TAAT AsnI 628 ATTAAT AT^TAAT Asp700I 1942 GAATACCTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC AspI 109 GACACGGTC GAC[ACGT]^[ACGT][ACGT]GTC AvaI 2776 CCCGGG C^[CT]CG[GA]G AviII 2396 TGCGCA TGC^GCA BanI 1119 GGTGCC G^G[CT][GA]CC BanII 1090 GAGCTC G[GA]GC[CT]^C BbvCI 805 GCTGAGG CCTCAGC(-5/-2) BciVI 540 GGATAC GTATCC(6/5) BclI 1506 TGATCA T^GATCA BfrI 1618 CTTAAG C^TTAAG BglII 2562 AGATCT A^GATCT BpmI 722 CTGGAG CTGGAG(16/14) BsaAI 2549 TACGTG [CT]AC^GT[GA] BsaWI 2464 ACCGGA [AT]^CCGG[AT] BsgI 2750 CTGCAC GTGCAG(16/14) BsiEI 113 CGGTCG CG[GA][CT]^CG BsmBI 1114 GAGACG CGTCTC(1/5) BsmI 2745 GCATTC GAATGC(1/-1) BsoBI 2776 CCCGGG C^[CT]CG[GA]G BspLU11I 570 ACATGT A^CATGT BspMI 2393 ACCTGC ACCTGC(4/8) BssSI 2666 CTCGTG CACGAG(-5/-1) BstAPI 2752 GCACAGATTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC Bsu36I 1729 CCTGAGG CC^T[ACGT]AGG Eco47III 2473 AGCGCT AGC^GCT Eco81I 1729 CCTGAGG CC^T[ACGT]AGG FbaI 1506 TGATCA T^GATCA FspI 2396 TGCGCA TGC^GCA HaeII 2473 AGCGCT [GA]GCGC^[CT] HpaI 1002 GTTAAC GTT^AAC KspI 72 CCGCGG CCGC^GG MfeI 1217 CAATTG C^AATTG MunI 1217 CAATTG C^AATTG PflFI 109 GACACGGTC GAC[ACGT]^[ACGT][ACGT]GTC PshBI 628 ATTAAT AT^TAAT Psp1406I 1817 AACGTT AA^CGTT PstI 1007 CTGCAG CTGCA^G SacI 1090 GAGCTC GAGCT^C SacII 72 CCGCGG CCGC^GG SmaI 2776 CCCGGG CCC^GGG SpeI 2010 ACTAGT A^CTAGT SspI 240 AATATT AAT^ATT Tth111I 109 GACACGGTC GAC[ACGT]^[ACGT][ACGT]GTC XmaCI 2776 CCCGGG C^CCGGG XmaI 2776 CCCGGG C^CCGGG XmnI 1942 GAATACCTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC ################################### # TWO CUT ENZYMES ( 44 enzymes) # ################################### AccII 73 CGCG CG^CG 123 CGCG Alw44I 501 GTGCAC G^TGCAC 2533 GTGCAC ApaLI 501 GTGCAC G^TGCAC 2533 GTGCAC BalI 954 TGGCCA TGG^CCA 2732 TGGCCA BcnI 2776 CCCGG CC^[GC]GG 2777 CCGGG BlpI 1414 GCTGAGC GC^T[ACGT]AGC 1603 GCTGAGC Bpu1102I 1414 GCTGAGC GC^T[ACGT]AGC 1603 GCTGAGC BsmFI 428 GGGAC GGGAC(10/14) 2774 GTCCC BspHI 297 TCATGA T^CATGA 465 TCATGA BstNI 9 CCTGG CC^[AT]GG 2418 CCTGG BstUI 73 CGCG CG^CG 123 CGCG CelII 1414 GCTGAGC GC^T[ACGT]AGC 1603 GCTGAGC CfoI 2397 GCGC GCG^C 2474 GCGC EcoRII 9 CCTGG ^CC[AT]GG 2418 CCTGG EcoT14I 362 CCATGG C^C[AT][AT]GG 1808 CCATGG EcoT22I 137 ATGCAT ATGCA^T 2660 ATGCAT HapII 2465 CCGG C^CGG 2777 CCGG HgaI 1350 GACGC GACGC(5/10) 124 GCGTC HhaI 2397 GCGC GCG^C 2474 GCGC HinP1I 2397 GCGC G^CGC 2474 GCGC HincII 1002 GTTAAC GT[CT]^[GA]AC 1328 GTCAAC HindII 1002 GTTAAC GT[CT]^[GA]AC 1328 GTCAAC HindIII 1163 AAGCTT A^AGCTT 2624 AAGCTT HpaII 2465 CCGG C^CGG 2777 CCGG HphI 1812 GGTGA GGTGA(8/7) 2805 TCACC MluNI 954 TGGCCA TGG^CCA 2732 TGGCCA MscI 954 TGGCCA TGG^CCA 2732 TGGCCA MspI 2465 CCGG C^CGG 2777 CCGG MvaI 9 CCTGG CC^[AT]GG 2418 CCTGG MvnI 73 CGCG CG^CG 123 CGCG NciI 2776 CCCGG CC^[GC]GG 2777 CCGGG NcoI 362 CCATGG C^CATGG 1808 CCATGG NdeI 248 CATATG CA^TATG 818 CATATG NlaIV 428 GGGACC GG[ACGT]^[ACGT]CC 1119 GGTGCC NsiI 137 ATGCAT ATGCA^T 2660 ATGCAT NspI 570 ACATGT [GA]CATG^[CT] 1891 GCATGT PflMI 2040 CCATGAAATGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG 2850 CCAGCCAGTGG PpuMI 428 GGGACCT [GA]G^G[AT]CC[CT] 2414 AGGACCT PvuII 1652 CAGCTG CAG^CTG 2609 CAGCTG RcaI 297 TCATGA T^CATGA 465 TCATGA SmlI 170 CTCAAG C^T[CT][GA]AG 1618 CTTAAG StyI 362 CCATGG C^C[AT][AT]GG 1808 CCATGG Tsp45I 611 GTCAC ^GT[GC]AC 768 GTCAC Van91I 2040 CCATGAAATGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG 2850 CCAGCCAGTGG ######################################### # 3 OR MORE CUT ENZYMES ( 67 enzymes) # ######################################### AciI AcsI AfaI AluI AlwI ApoI AspHI AvaII BbsI BbvI BfaI BmyI BpuAI BsaJI BseRI BsiHKAI BsiYI BslI BsmAI Bsp1286I BsrI BssKI BstF5I BstYI Cac8I Cfr13I DdeI DpnII DraII DsaI EaeI EarI EcoO109I Fnu4HI FokI HaeIII HinfI ItaI Ksp632I MaeI MaeII MaeIII MboI MboII MflI MnlI MseI MslI MspA1I MwoI NdeII NlaIII PleI RsaI Sau3AI Sau96I ScrFI SfaNI SfcI TaqI TfiI Tru9I TseI Tsp509I TspRI TthHB8I XhoII