Restriction map for
pFN21AB8531
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# NO CUT ENZYMES ( 93 enzymes) #
##################################
AatII Acc65I AccI AccIII AcyI
AgeI AhdI ApaI AscI Asp718I
AspEI AvrII BamHI BbeI BbrPI
BcgI BglI BlnI BsaBI BsaHI
BsaI BseAI BsiWI Bsp1407I BspDI
BspEI BsrBI BsrDI BsrFI BsrGI
BssHII Bst1107I BstBI BstEII BstPI
BstXI BstZ17I Cfr10I ClaI CpoI
DraI DraIII DrdI EagI Eam1105I
EclXI Eco52I EcoNI EcoO65I EcoRI
EcoRV FauI FseI Hin1I KasI
KpnI MamI MluI MroI NaeI
NarI NgoMIV NheI NotI NruI
NspV PacI PaeR7I PinAI PmaCI
PmeI PmlI PshAI PvuI RsrII
SalI SapI SbfI ScaI SexAI
SfiI SfoI SfuI SgrAI SnaBI
SphI Sse8387I SspBI StuI SwaI
XbaI XcmI XhoI
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# ONE CUT ENZYMES ( 54 enzymes) #
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AclI 1817 AACGTT AA^CGTT
AflII 1618 CTTAAG C^TTAAG
AflIII 570 ACATGT A^C[GA][CT]GT
AlwNI 2413 CAGGACCTG CAG[ACGT][ACGT][ACGT]^CTG
AocI 1729 CCTGAGG CC^T[ACGT]AGG
Aor51HI 2473 AGCGCT AGC^GCT
AseI 628 ATTAAT AT^TAAT
AsnI 628 ATTAAT AT^TAAT
Asp700I 1942 GAATACCTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
AspI 109 GACACGGTC GAC[ACGT]^[ACGT][ACGT]GTC
AvaI 2776 CCCGGG C^[CT]CG[GA]G
AviII 2396 TGCGCA TGC^GCA
BanI 1119 GGTGCC G^G[CT][GA]CC
BanII 1090 GAGCTC G[GA]GC[CT]^C
BbvCI 805 GCTGAGG CCTCAGC(-5/-2)
BciVI 540 GGATAC GTATCC(6/5)
BclI 1506 TGATCA T^GATCA
BfrI 1618 CTTAAG C^TTAAG
BglII 2562 AGATCT A^GATCT
BpmI 722 CTGGAG CTGGAG(16/14)
BsaAI 2549 TACGTG [CT]AC^GT[GA]
BsaWI 2464 ACCGGA [AT]^CCGG[AT]
BsgI 2750 CTGCAC GTGCAG(16/14)
BsiEI 113 CGGTCG CG[GA][CT]^CG
BsmBI 1114 GAGACG CGTCTC(1/5)
BsmI 2745 GCATTC GAATGC(1/-1)
BsoBI 2776 CCCGGG C^[CT]CG[GA]G
BspLU11I 570 ACATGT A^CATGT
BspMI 2393 ACCTGC ACCTGC(4/8)
BssSI 2666 CTCGTG CACGAG(-5/-1)
BstAPI 2752 GCACAGATTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
Bsu36I 1729 CCTGAGG CC^T[ACGT]AGG
Eco47III 2473 AGCGCT AGC^GCT
Eco81I 1729 CCTGAGG CC^T[ACGT]AGG
FbaI 1506 TGATCA T^GATCA
FspI 2396 TGCGCA TGC^GCA
HaeII 2473 AGCGCT [GA]GCGC^[CT]
HpaI 1002 GTTAAC GTT^AAC
KspI 72 CCGCGG CCGC^GG
MfeI 1217 CAATTG C^AATTG
MunI 1217 CAATTG C^AATTG
PflFI 109 GACACGGTC GAC[ACGT]^[ACGT][ACGT]GTC
PshBI 628 ATTAAT AT^TAAT
Psp1406I 1817 AACGTT AA^CGTT
PstI 1007 CTGCAG CTGCA^G
SacI 1090 GAGCTC GAGCT^C
SacII 72 CCGCGG CCGC^GG
SmaI 2776 CCCGGG CCC^GGG
SpeI 2010 ACTAGT A^CTAGT
SspI 240 AATATT AAT^ATT
Tth111I 109 GACACGGTC GAC[ACGT]^[ACGT][ACGT]GTC
XmaCI 2776 CCCGGG C^CCGGG
XmaI 2776 CCCGGG C^CCGGG
XmnI 1942 GAATACCTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
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# TWO CUT ENZYMES ( 44 enzymes) #
###################################
AccII 73 CGCG CG^CG
123 CGCG
Alw44I 501 GTGCAC G^TGCAC
2533 GTGCAC
ApaLI 501 GTGCAC G^TGCAC
2533 GTGCAC
BalI 954 TGGCCA TGG^CCA
2732 TGGCCA
BcnI 2776 CCCGG CC^[GC]GG
2777 CCGGG
BlpI 1414 GCTGAGC GC^T[ACGT]AGC
1603 GCTGAGC
Bpu1102I 1414 GCTGAGC GC^T[ACGT]AGC
1603 GCTGAGC
BsmFI 428 GGGAC GGGAC(10/14)
2774 GTCCC
BspHI 297 TCATGA T^CATGA
465 TCATGA
BstNI 9 CCTGG CC^[AT]GG
2418 CCTGG
BstUI 73 CGCG CG^CG
123 CGCG
CelII 1414 GCTGAGC GC^T[ACGT]AGC
1603 GCTGAGC
CfoI 2397 GCGC GCG^C
2474 GCGC
EcoRII 9 CCTGG ^CC[AT]GG
2418 CCTGG
EcoT14I 362 CCATGG C^C[AT][AT]GG
1808 CCATGG
EcoT22I 137 ATGCAT ATGCA^T
2660 ATGCAT
HapII 2465 CCGG C^CGG
2777 CCGG
HgaI 1350 GACGC GACGC(5/10)
124 GCGTC
HhaI 2397 GCGC GCG^C
2474 GCGC
HinP1I 2397 GCGC G^CGC
2474 GCGC
HincII 1002 GTTAAC GT[CT]^[GA]AC
1328 GTCAAC
HindII 1002 GTTAAC GT[CT]^[GA]AC
1328 GTCAAC
HindIII 1163 AAGCTT A^AGCTT
2624 AAGCTT
HpaII 2465 CCGG C^CGG
2777 CCGG
HphI 1812 GGTGA GGTGA(8/7)
2805 TCACC
MluNI 954 TGGCCA TGG^CCA
2732 TGGCCA
MscI 954 TGGCCA TGG^CCA
2732 TGGCCA
MspI 2465 CCGG C^CGG
2777 CCGG
MvaI 9 CCTGG CC^[AT]GG
2418 CCTGG
MvnI 73 CGCG CG^CG
123 CGCG
NciI 2776 CCCGG CC^[GC]GG
2777 CCGGG
NcoI 362 CCATGG C^CATGG
1808 CCATGG
NdeI 248 CATATG CA^TATG
818 CATATG
NlaIV 428 GGGACC GG[ACGT]^[ACGT]CC
1119 GGTGCC
NsiI 137 ATGCAT ATGCA^T
2660 ATGCAT
NspI 570 ACATGT [GA]CATG^[CT]
1891 GCATGT
PflMI 2040 CCATGAAATGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
2850 CCAGCCAGTGG
PpuMI 428 GGGACCT [GA]G^G[AT]CC[CT]
2414 AGGACCT
PvuII 1652 CAGCTG CAG^CTG
2609 CAGCTG
RcaI 297 TCATGA T^CATGA
465 TCATGA
SmlI 170 CTCAAG C^T[CT][GA]AG
1618 CTTAAG
StyI 362 CCATGG C^C[AT][AT]GG
1808 CCATGG
Tsp45I 611 GTCAC ^GT[GC]AC
768 GTCAC
Van91I 2040 CCATGAAATGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
2850 CCAGCCAGTGG
#########################################
# 3 OR MORE CUT ENZYMES ( 67 enzymes) #
#########################################
AciI AcsI AfaI AluI AlwI
ApoI AspHI AvaII BbsI BbvI
BfaI BmyI BpuAI BsaJI BseRI
BsiHKAI BsiYI BslI BsmAI Bsp1286I
BsrI BssKI BstF5I BstYI Cac8I
Cfr13I DdeI DpnII DraII DsaI
EaeI EarI EcoO109I Fnu4HI FokI
HaeIII HinfI ItaI Ksp632I MaeI
MaeII MaeIII MboI MboII MflI
MnlI MseI MslI MspA1I MwoI
NdeII NlaIII PleI RsaI Sau3AI
Sau96I ScrFI SfaNI SfcI TaqI
TfiI Tru9I TseI Tsp509I TspRI
TthHB8I XhoII