Restriction map for
pFN21AE5250
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# NO CUT ENZYMES ( 172 enzymes) #
##################################
AatII Acc65I AccI AccIII AclI
AcsI AfaI AflII AflIII AgeI
AhdI Alw44I AocI Aor51HI ApaI
ApaLI ApoI AscI AseI AsnI
Asp700I Asp718I AspEI AspHI AspI
AviII AvrII BalI BamHI BanII
BbrPI BbsI BbvCI BcgI BciVI
BclI BcnI BfaI BfrI BglII
BlnI BmyI BpmI BpuAI BsaAI
BsaBI BsaWI BseAI BseRI BsiEI
BsiHKAI BsiWI BsiYI BslI BsmBI
BsmFI Bsp1286I Bsp1407I BspDI BspEI
BspHI BspLU11I BspMI BsrDI BsrFI
BsrGI BssHII BssSI Bst1107I BstAPI
BstBI BstEII BstPI BstXI BstZ17I
Bsu36I Cfr10I ClaI CpoI DraI
DraII DraIII DrdI DsaI EaeI
EagI Eam1105I EarI EclXI Eco47III
Eco52I Eco81I EcoNI EcoO109I EcoO65I
EcoRI EcoRV EcoT22I FbaI FseI
FspI HincII HindII HindIII HpaI
KpnI Ksp632I KspI MaeI MamI
MfeI MluI MluNI MroI MscI
MslI MspA1I MunI NaeI NciI
NcoI NdeI NgoMIV NheI NotI
NruI NsiI NspI NspV PacI
PflFI PflMI PinAI PmaCI PmeI
PmlI PpuMI PshAI PshBI Psp1406I
PstI PvuI PvuII RcaI RsaI
RsrII SacI SacII SalI SapI
SbfI ScaI SexAI SfcI SfiI
SfuI SgrAI SmaI SnaBI SpeI
SphI Sse8387I SspBI StuI SwaI
Tth111I Van91I XbaI XcmI XmaCI
XmaI XmnI
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# ONE CUT ENZYMES ( 44 enzymes) #
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AcyI 184 GGCGCC G[GA]^CG[CT]C
AlwI 278 GGATC GGATC(4/5)
AlwNI 77 CAGCCTCTG CAG[ACGT][ACGT][ACGT]^CTG
AvaI 231 CTCGAG C^[CT]CG[GA]G
AvaII 327 GGTCC G^G[AT]CC
BbeI 184 GGCGCC GGCGC^C
BglI 79 GCCTCTGCGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
BlpI 431 GCTCAGC GC^T[ACGT]AGC
Bpu1102I 431 GCTCAGC GC^T[ACGT]AGC
BsaHI 184 GGCGCC G[GA]^CG[CT]C
BsaI 545 GAGACC GGTCTC(1/5)
BsgI 384 GTGCAG GTGCAG(16/14)
BsmI 152 GCATTC GAATGC(1/-1)
BsoBI 231 CTCGAG C^[CT]CG[GA]G
BsrBI 300 GAGCGG CCGCTC(-3/-3)
BstYI 278 GGATCT [GA]^GATC[CT]
CelII 431 GCTCAGC GC^T[ACGT]AGC
CfoI 185 GCGC GCG^C
Cfr13I 327 GGTCC G^G[ACGT]CC
FauI 148 GCGGG CCCGC(4/6)
HaeII 184 GGCGCC [GA]GCGC^[CT]
HaeIII 241 GGCC GG^CC
HapII 123 CCGG C^CGG
HhaI 185 GCGC GCG^C
Hin1I 184 GGCGCC G[GA]^CG[CT]C
HinP1I 185 GCGC G^CGC
HpaII 123 CCGG C^CGG
HphI 28 TCACC GGTGA(8/7)
KasI 184 GGCGCC G^GCGCC
MaeII 422 ACGT A^CGT
MflI 278 GGATCT [GA]^GATC[CT]
MseI 488 TTAA T^TAA
MspI 123 CCGG C^CGG
NarI 184 GGCGCC GG^CGCC
NlaIII 285 CATG CATG^
PaeR7I 231 CTCGAG C^TCGAG
Sau96I 327 GGTCC G^G[ACGT]CC
SfoI 184 GGCGCC GGC^GCC
SmlI 231 CTCGAG C^T[CT][GA]AG
SspI 225 AATATT AAT^ATT
TfiI 99 GAATC G^A[AT]TC
Tru9I 488 TTAA T^TAA
XhoI 231 CTCGAG C^TCGAG
XhoII 278 GGATCT [GA]^GATC[CT]
###################################
# TWO CUT ENZYMES ( 19 enzymes) #
###################################
AccII 378 CGCG CG^CG
391 CGCG
BanI 91 GGCACC G^G[CT][GA]CC
184 GGCGCC
BsaJI 267 CCTTGG C^C[ACGT][ACGT]GG
437 CCTTGG
BstF5I 137 GGATG GGATG(2/0)
333 GGATG
BstUI 378 CGCG CG^CG
391 CGCG
DpnII 279 GATC ^GATC
366 GATC
EcoT14I 267 CCTTGG C^C[AT][AT]GG
437 CCTTGG
FokI 137 GGATG GGATG(9/13)
333 GGATG
HgaI 7 GACGC GACGC(5/10)
389 GACGC
MboI 279 GATC ^GATC
366 GATC
MvnI 378 CGCG CG^CG
391 CGCG
MwoI 79 GCCTCTGCGGC GC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GC
427 GCCTGCTCAGC
NdeII 279 GATC ^GATC
366 GATC
NlaIV 91 GGCACC GG[ACGT]^[ACGT]CC
184 GGCGCC
PleI 61 GACTC GAGTC(4/5)
190 GAGTC
Sau3AI 279 GATC ^GATC
366 GATC
StyI 267 CCTTGG C^C[AT][AT]GG
437 CCTTGG
TaqI 232 TCGA T^CGA
347 TCGA
TthHB8I 232 TCGA T^CGA
347 TCGA
#########################################
# 3 OR MORE CUT ENZYMES ( 23 enzymes) #
#########################################
AciI AluI BbvI BsmAI BsrI
BssKI BstNI Cac8I DdeI EcoRII
Fnu4HI HinfI ItaI MaeIII MboII
MnlI MvaI ScrFI SfaNI TseI
Tsp45I Tsp509I TspRI