Restriction map for
pFN21AE2230
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# NO CUT ENZYMES ( 151 enzymes) #
##################################
AatII Acc65I AccII AccIII AclI
AcyI AgeI AhdI Aor51HI AscI
AseI AsnI Asp700I Asp718I AspEI
AspI AvaI AviII AvrII BalI
BamHI BanI BbeI BbvCI BcgI
BciVI BcnI BglI BglII BlnI
BlpI Bpu1102I BsaBI BsaHI BseAI
BsiEI BsiWI BsmBI BsoBI Bsp1407I
BspDI BspEI BspHI BspMI BsrBI
BsrFI BsrGI BssHII BssSI BstAPI
BstBI BstEII BstPI BstUI BstXI
BstYI Cac8I CelII CfoI Cfr10I
ClaI CpoI DraI DraIII DrdI
DsaI EagI Eam1105I EclXI Eco47III
Eco52I EcoNI EcoO65I EcoT14I FauI
FseI FspI HaeII HhaI Hin1I
HinP1I HincII HindII HindIII HpaI
HphI KasI KpnI KspI MamI
MfeI MflI MluI MluNI MroI
MscI MunI MvnI NaeI NarI
NciI NcoI NdeI NgoMIV NheI
NotI NruI NspV PacI PaeR7I
PflFI PflMI PinAI PmeI PpuMI
PshAI PshBI Psp1406I PvuI RcaI
RsrII SacI SacII SalI SapI
SbfI ScaI SexAI SfiI SfoI
SfuI SgrAI SmaI SnaBI SpeI
SphI Sse8387I SspBI StuI StyI
SwaI TaqI Tth111I TthHB8I Van91I
XcmI XhoI XhoII XmaCI XmaI
XmnI
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# ONE CUT ENZYMES ( 43 enzymes) #
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AccI 448 GTATAC GT^[AC][GT]AC
AflII 49 CTTAAG C^TTAAG
Alw44I 1116 GTGCAC G^TGCAC
AlwI 1273 GATCC GGATC(4/5)
AocI 31 CCTGAGG CC^T[ACGT]AGG
ApaI 1157 GGGCCC GGGCC^C
ApaLI 1116 GTGCAC G^TGCAC
AspHI 1116 GTGCAC G[AT]GC[AT]^C
AvaII 304 GGACC G^G[AT]CC
BbrPI 1113 CACGTG CAC^GTG
BbsI 1333 GAAGAC GAAGAC(2/6)
BclI 771 TGATCA T^GATCA
BfaI 715 CTAG C^TAG
BfrI 49 CTTAAG C^TTAAG
BpuAI 1333 GAAGAC GAAGAC(2/6)
BsaAI 1113 CACGTG [CT]AC^GT[GA]
BsaWI 1293 ACCGGA [AT]^CCGG[AT]
BsgI 725 GTGCAG GTGCAG(16/14)
BsiHKAI 1116 GTGCAC G[AT]GC[AT]^C
BsmFI 987 GGGAC GGGAC(10/14)
BsmI 831 GAATGC GAATGC(1/-1)
Bst1107I 448 GTATAC GTA^TAC
BstZ17I 448 GTATAC GTA^TAC
Bsu36I 31 CCTGAGG CC^T[ACGT]AGG
DraII 1157 GGGCCCT [GA]G^G[ACGT]CC[CT]
EaeI 1130 TGGCCG [CT]^GGCC[GA]
Eco81I 31 CCTGAGG CC^T[ACGT]AGG
EcoO109I 1157 GGGCCCT [GA]G^G[ACGT]CC[CT]
EcoRV 18 GATATC GAT^ATC
EcoT22I 766 ATGCAT ATGCA^T
FbaI 771 TGATCA T^GATCA
HapII 1294 CCGG C^CGG
HgaI 1344 GCGTC GACGC(5/10)
HpaII 1294 CCGG C^CGG
MaeI 715 CTAG C^TAG
MaeII 1114 ACGT A^CGT
MspI 1294 CCGG C^CGG
NsiI 766 ATGCAT ATGCA^T
PmaCI 1113 CACGTG CAC^GTG
PmlI 1113 CACGTG CAC^GTG
SmlI 49 CTTAAG C^T[CT][GA]AG
SspI 316 AATATT AAT^ATT
XbaI 714 TCTAGA T^CTAGA
###################################
# TWO CUT ENZYMES ( 21 enzymes) #
###################################
AflIII 195 ACATGT A^C[GA][CT]GT
1337 ACATGT
AlwNI 728 CAGCACCTG CAG[ACGT][ACGT][ACGT]^CTG
1078 CAGCTGCTG
BsaI 27 GAGACC GGTCTC(1/5)
993 GAGACC
BseRI 377 GAGGAG GAGGAG(10/8)
1211 GAGGAG
BsmAI 27 GAGAC GTCTC(1/5)
993 GAGAC
BspLU11I 195 ACATGT A^CATGT
1337 ACATGT
BsrDI 753 CATTGC GCAATG(2/0)
362 GCAATG
DpnII 772 GATC ^GATC
1273 GATC
EarI 1060 CTCTTC CTCTTC(1/4)
1328 GAAGAG
EcoRI 852 GAATTC G^AATTC
956 GAATTC
Ksp632I 1060 CTCTTC CTCTTC(1/4)
1328 GAAGAG
MboI 772 GATC ^GATC
1273 GATC
MwoI 1044 GCCATTACAGC GC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GC
1080 GCTGCTGAAGC
NdeII 772 GATC ^GATC
1273 GATC
NspI 195 ACATGT [GA]CATG^[CT]
1337 ACATGT
PleI 1309 GACTC GAGTC(4/5)
1360 GACTC
PstI 1067 CTGCAG CTGCA^G
1261 CTGCAG
PvuII 1078 CAGCTG CAG^CTG
1258 CAGCTG
Sau3AI 772 GATC ^GATC
1273 GATC
TfiI 711 GATTC G^A[AT]TC
943 GAATC
Tsp45I 79 GTGAC ^GT[GC]AC
138 GTCAC
#########################################
# 3 OR MORE CUT ENZYMES ( 43 enzymes) #
#########################################
AciI AcsI AfaI AluI ApoI
BanII BbvI BmyI BpmI BsaJI
BsiYI BslI Bsp1286I BsrI BssKI
BstF5I BstNI Cfr13I DdeI EcoRII
Fnu4HI FokI HaeIII HinfI ItaI
MaeIII MboII MnlI MseI MslI
MspA1I MvaI NlaIII NlaIV RsaI
Sau96I ScrFI SfaNI SfcI Tru9I
TseI Tsp509I TspRI