Restriction map for
pFN21AE1870
##################################
# NO CUT ENZYMES ( 146 enzymes) #
##################################
AatII Acc65I AccIII AcyI AflII
AflIII AgeI AhdI Alw44I AlwI
AocI Aor51HI ApaI ApaLI AscI
AseI AsnI Asp718I AspEI AspHI
AvaI AviII AvrII BalI BamHI
BanI BbeI BbrPI BbsI BbvCI
BciVI BclI BcnI BfrI BglI
BglII BlnI BlpI Bpu1102I BpuAI
BsaAI BsaHI BsaWI BseAI BseRI
BsiEI BsiHKAI BsiWI BsmBI BsoBI
BspDI BspEI BspHI BspLU11I BsrBI
BssHII BssSI BstAPI BstBI BstEII
BstPI BstXI BstYI Bsu36I CelII
ClaI CpoI DraI DraII DraIII
DrdI DsaI EagI Eam1105I EclXI
Eco47III Eco52I Eco81I EcoNI EcoO109I
EcoO65I EcoRI EcoRV EcoT22I FbaI
FseI FspI HaeII HgaI Hin1I
HpaI KasI KpnI KspI MflI
MluI MluNI MroI MscI NarI
NciI NcoI NdeI NheI NotI
NruI NsiI NspV PacI PaeR7I
PflMI PinAI PleI PmaCI PmeI
PmlI PpuMI PshAI PshBI PvuI
PvuII RcaI RsrII SacI SacII
SalI SbfI ScaI SexAI SfiI
SfoI SfuI SgrAI SmaI SmlI
SnaBI SpeI Sse8387I StuI SwaI
Van91I XcmI XhoI XhoII XmaCI
XmaI
###################################
# ONE CUT ENZYMES ( 62 enzymes) #
###################################
AccII 54 CGCG CG^CG
AclI 625 AACGTT AA^CGTT
AlwNI 911 CAGGGTCTG CAG[ACGT][ACGT][ACGT]^CTG
AspI 909 GACAGGGTC GAC[ACGT]^[ACGT][ACGT]GTC
AvaII 866 GGTCC G^G[AT]CC
BcgI 176 GCATTACACTCG (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
BfaI 307 CTAG C^TAG
BsaBI 733 GATAGTGATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
BsaI 389 GAGACC GGTCTC(1/5)
BsmFI 907 GGGAC GGGAC(10/14)
BsmI 464 GCATTC GAATGC(1/-1)
Bsp1407I 573 TGTACA T^GTACA
BsrFI 257 GCCGGC [GA]^CCGG[CT]
BsrGI 573 TGTACA T^GTACA
BsrI 792 CCAGT ACTGG(1/-1)
BssKI 535 CCTGG ^CC[ACGT]GG
BstNI 535 CCTGG CC^[AT]GG
BstUI 54 CGCG CG^CG
CfoI 55 GCGC GCG^C
Cfr10I 257 GCCGGC [GA]^CCGG[CT]
Cfr13I 866 GGTCC G^G[ACGT]CC
DpnII 739 GATC ^GATC
EaeI 255 TGGCCG [CT]^GGCC[GA]
EcoRII 535 CCTGG ^CC[AT]GG
EcoT14I 1053 CCTTGG C^C[AT][AT]GG
FauI 12 GCGGG CCCGC(4/6)
HaeIII 256 GGCC GG^CC
HapII 258 CCGG C^CGG
HhaI 55 GCGC GCG^C
HinP1I 55 GCGC G^CGC
HincII 103 GTCAAC GT[CT]^[GA]AC
HindII 103 GTCAAC GT[CT]^[GA]AC
HpaII 258 CCGG C^CGG
HphI 265 GGTGA GGTGA(8/7)
MaeI 307 CTAG C^TAG
MamI 733 GATAGTGATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
MboI 739 GATC ^GATC
MfeI 167 CAATTG C^AATTG
MslI 780 CACTGACGTG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
MspA1I 30 CAGCGG C[AC]G^C[GT]G
MspI 258 CCGG C^CGG
MunI 167 CAATTG C^AATTG
MvaI 535 CCTGG CC^[AT]GG
MvnI 54 CGCG CG^CG
NaeI 257 GCCGGC GCC^GGC
NdeII 739 GATC ^GATC
NgoMIV 257 GCCGGC G^CCGGC
NlaIV 3 GGAGCC GG[ACGT]^[ACGT]CC
PflFI 909 GACAGGGTC GAC[ACGT]^[ACGT][ACGT]GTC
Psp1406I 625 AACGTT AA^CGTT
SapI 829 GCTCTTC GCTCTTC(1/4)
Sau3AI 739 GATC ^GATC
Sau96I 866 GGTCC G^G[ACGT]CC
ScrFI 535 CCTGG CC^[ACGT]GG
SfaNI 676 GATGC GCATC(5/9)
SphI 44 GCATGC GCATG^C
SspBI 573 TGTACA T^GTACA
SspI 637 AATATT AAT^ATT
StyI 1053 CCTTGG C^C[AT][AT]GG
Tsp45I 978 GTCAC ^GT[GC]AC
Tth111I 909 GACAGGGTC GAC[ACGT]^[ACGT][ACGT]GTC
XbaI 306 TCTAGA T^CTAGA
###################################
# TWO CUT ENZYMES ( 28 enzymes) #
###################################
AccI 71 GTATAC GT^[AC][GT]AC
124 GTATAC
Asp700I 288 GAATAGCTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
706 GAAGAAATTC
BanII 4 GAGCCC G[GA]GC[CT]^C
225 GAGCCC
BmyI 4 GAGCCC G[AGT]GC[ACT]^C
225 GAGCCC
BpmI 1064 CTCCAG CTGGAG(16/14)
19 CTGGAG
BsaJI 534 CCCTGG C^C[ACGT][ACGT]GG
1053 CCTTGG
BsgI 77 CTGCAC GTGCAG(16/14)
940 GTGCAG
BsiYI 534 CCCTGGTCAGG CC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GG
1066 CCAGAGTTCGG
BslI 534 CCCTGGTCAGG CC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GG
1066 CCAGAGTTCGG
BsmAI 389 GAGAC GTCTC(1/5)
1127 GTCTC
Bsp1286I 4 GAGCCC G[AGT]GC[ACT]^C
225 GAGCCC
BspMI 75 ACCTGC ACCTGC(4/8)
942 GCAGGT
BsrDI 815 CATTGC GCAATG(2/0)
592 GCAATG
Bst1107I 71 GTATAC GTA^TAC
124 GTATAC
BstZ17I 71 GTATAC GTA^TAC
124 GTATAC
DdeI 58 CTGAG C^T[ACGT]AG
1082 CTGAG
EarI 830 CTCTTC CTCTTC(1/4)
187 GAAGAG
HindIII 502 AAGCTT A^AGCTT
691 AAGCTT
HinfI 277 GATTC G^A[ACGT]TC
568 GAATC
Ksp632I 830 CTCTTC CTCTTC(1/4)
187 GAAGAG
NlaIII 45 CATG CATG^
1138 CATG
NspI 44 GCATGC [GA]CATG^[CT]
1137 GCATGT
PstI 701 CTGCAG CTGCA^G
929 CTGCAG
SfcI 701 CTGCAG C^T[GA][CT]AG
929 CTGCAG
TaqI 185 TCGA T^CGA
209 TCGA
TfiI 277 GATTC G^A[AT]TC
568 GAATC
TthHB8I 185 TCGA T^CGA
209 TCGA
XmnI 288 GAATAGCTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
706 GAAGAAATTC
#########################################
# 3 OR MORE CUT ENZYMES ( 22 enzymes) #
#########################################
AciI AcsI AfaI AluI ApoI
BbvI BstF5I Cac8I Fnu4HI FokI
ItaI MaeII MaeIII MboII MnlI
MseI MwoI RsaI Tru9I TseI
Tsp509I TspRI