Restriction map for
pFN21AE1771
##################################
# NO CUT ENZYMES ( 165 enzymes) #
##################################
AatII Acc65I AccI AccII AccIII
AclI AcyI AflII AflIII AgeI
AhdI AlwI AlwNI Aor51HI ApaI
AscI AseI AsnI Asp700I Asp718I
AspEI AspI AviII AvrII BamHI
BbeI BbrPI BbsI BcgI BciVI
BclI BfrI BglI BglII BlnI
BlpI BpmI Bpu1102I BpuAI BsaAI
BsaBI BsaHI BsaI BsaWI BseAI
BsgI BsiEI BsiWI BsmBI BsmI
Bsp1407I BspDI BspEI BspHI BspLU11I
BsrBI BsrFI BsrGI BssHII Bst1107I
BstAPI BstBI BstUI BstYI BstZ17I
CelII CfoI Cfr10I ClaI CpoI
DraI DraII DraIII DrdI DsaI
EagI Eam1105I EclXI Eco47III Eco52I
EcoNI EcoO109I EcoRI EcoRV EcoT22I
FauI FbaI FseI FspI HaeII
HgaI HhaI Hin1I HinP1I HindIII
HinfI HpaI KasI KpnI KspI
MamI MfeI MflI MluI MroI
MslI MspA1I MunI MvnI NaeI
NarI NcoI NdeI NgoMIV NheI
NotI NruI NsiI NspV PacI
PaeR7I PflFI PinAI PleI PmaCI
PmeI PmlI PpuMI PshAI PshBI
Psp1406I PstI PvuI PvuII RcaI
RsrII SacII SalI SapI SbfI
ScaI SexAI SfcI SfiI SfoI
SfuI SgrAI SmaI SnaBI SpeI
SphI Sse8387I SspBI SspI StuI
SwaI TfiI TspRI Tth111I XbaI
XhoI XhoII XmaCI XmaI XmnI
###################################
# ONE CUT ENZYMES ( 40 enzymes) #
###################################
AfaI 351 GTAC GT^AC
Alw44I 410 GTGCAC G^TGCAC
ApaLI 410 GTGCAC G^TGCAC
AvaI 52 CCCGAG C^[CT]CG[GA]G
AvaII 135 GGTCC G^G[AT]CC
BalI 615 TGGCCA TGG^CCA
BanI 162 GGTGCC G^G[CT][GA]CC
BanII 550 GAGCTC G[GA]GC[CT]^C
BbvCI 138 CCTCAGC CCTCAGC(-5/-2)
BfaI 595 CTAG C^TAG
BsmFI 690 GTCCC GGGAC(10/14)
BsoBI 52 CCCGAG C^[CT]CG[GA]G
BspMI 624 GCAGGT ACCTGC(4/8)
BsrDI 663 CATTGC GCAATG(2/0)
BssSI 305 CACGAG CACGAG(-5/-1)
BstEII 654 GGTGACC G^GT[ACGT]ACC
BstF5I 93 GGATG GGATG(2/0)
BstPI 654 GGTGACC G^GT[ACGT]ACC
BstXI 83 CCAACATGCTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
Cfr13I 135 GGTCC G^G[ACGT]CC
EaeI 615 TGGCCA [CT]^GGCC[GA]
EarI 374 CTCTTC CTCTTC(1/4)
EcoO65I 654 GGTGACC G^GT[ACGT]ACC
EcoT14I 276 CCTTGG C^C[AT][AT]GG
FokI 93 GGATG GGATG(9/13)
HincII 97 GTCAAC GT[CT]^[GA]AC
HindII 97 GTCAAC GT[CT]^[GA]AC
Ksp632I 374 CTCTTC CTCTTC(1/4)
MaeI 595 CTAG C^TAG
MaeII 349 ACGT A^CGT
MluNI 615 TGGCCA TGG^CCA
MscI 615 TGGCCA TGG^CCA
PflMI 451 CCAGATATTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
RsaI 351 GTAC GT^AC
SacI 550 GAGCTC GAGCT^C
Sau96I 135 GGTCC G^G[ACGT]CC
SfaNI 77 GCATC GCATC(5/9)
StyI 276 CCTTGG C^C[AT][AT]GG
Van91I 451 CCAGATATTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
XcmI 672 CCAGTTCCAGCTTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
###################################
# TWO CUT ENZYMES ( 17 enzymes) #
###################################
AciI 448 CCGC CCGC(-3/-1)
570 CCGC
AcsI 189 GAATTT [GA]^AATT[CT]
369 AAATTC
AocI 489 CCTTAGG CC^T[ACGT]AGG
517 CCTAAGG
ApoI 189 GAATTT [GA]^AATT[CT]
369 AAATTC
BcnI 36 CCGGG CC^[GC]GG
72 CCGGG
BseRI 28 GAGGAG GAGGAG(10/8)
226 GAGGAG
BsmAI 718 GAGAC GTCTC(1/5)
9 GTCTC
Bsu36I 489 CCTTAGG CC^T[ACGT]AGG
517 CCTAAGG
Eco81I 489 CCTTAGG CC^T[ACGT]AGG
517 CCTAAGG
HphI 654 GGTGA GGTGA(8/7)
575 TCACC
MwoI 534 GCTTACCCAGC GC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GC
543 GCTTAAAGAGC
NciI 36 CCGGG CC^[GC]GG
72 CCGGG
NspI 86 ACATGC [GA]CATG^[CT]
746 ACATGC
SmlI 331 CTTGAG C^T[CT][GA]AG
360 CTTGAG
TaqI 224 TCGA T^CGA
790 TCGA
Tsp509I 190 AATT ^AATT
370 AATT
TthHB8I 224 TCGA T^CGA
790 TCGA
#########################################
# 3 OR MORE CUT ENZYMES ( 36 enzymes) #
#########################################
AluI AspHI BbvI BmyI BsaJI
BsiHKAI BsiYI BslI Bsp1286I BsrI
BssKI BstNI Cac8I DdeI DpnII
EcoRII Fnu4HI HaeIII HapII HpaII
ItaI MaeIII MboI MboII MnlI
MseI MspI MvaI NdeII NlaIII
NlaIV Sau3AI ScrFI Tru9I TseI
Tsp45I