Restriction map for
pFN21AB8520
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# NO CUT ENZYMES ( 97 enzymes) #
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AatII Acc65I AccI AccIII AclI
AgeI ApaI AscI AseI AsnI
Asp718I AspI AviII AvrII BbeI
BbrPI BcgI BglII BlnI BsaAI
BsaBI BsaWI BseAI BsgI BsiWI
Bsp1407I BspEI BspHI BspLU11I BsrBI
BsrDI BsrGI BssHII BssSI Bst1107I
BstBI BstEII BstPI BstZ17I CpoI
DraI DrdI EcoO65I EcoRV EcoT22I
FseI FspI HindIII HpaI KasI
KpnI KspI MamI MluI MroI
NaeI NarI NcoI NgoMIV NheI
NotI NruI NsiI NspV PacI
PflFI PflMI PinAI PmaCI PmeI
PmlI PshBI Psp1406I PvuI RcaI
RsrII SacII SalI SbfI SexAI
SfiI SfoI SfuI SgrAI SmaI
SnaBI SpeI SphI Sse8387I SspBI
SspI SwaI Tth111I Van91I XbaI
XmaCI XmaI
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# ONE CUT ENZYMES ( 43 enzymes) #
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AccII 98 CGCG CG^CG
AcyI 1869 GACGCC G[GA]^CG[CT]C
AflII 475 CTTAAG C^TTAAG
AhdI 16 GACAATCTGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
AocI 2059 CCTCAGG CC^T[ACGT]AGG
Asp700I 262 GAAGAAGTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
AspEI 16 GACAATCTGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
AvaI 770 CTCGAG C^[CT]CG[GA]G
BanI 1336 GGCACC G^G[CT][GA]CC
BciVI 775 GTATCC GTATCC(6/5)
BfrI 475 CTTAAG C^TTAAG
BglI 1361 GCCAAGATGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
BsaHI 1869 GACGCC G[GA]^CG[CT]C
BsaI 2380 GAGACC GGTCTC(1/5)
BsiEI 807 CGGCCG CG[GA][CT]^CG
BsmBI 203 GAGACG CGTCTC(1/5)
BsmI 2531 GAATGC GAATGC(1/-1)
BsoBI 770 CTCGAG C^[CT]CG[GA]G
BspDI 1111 ATCGAT AT^CGAT
BsrFI 805 ACCGGC [GA]^CCGG[CT]
BstUI 98 CGCG CG^CG
Bsu36I 2059 CCTCAGG CC^T[ACGT]AGG
Cfr10I 805 ACCGGC [GA]^CCGG[CT]
ClaI 1111 ATCGAT AT^CGAT
DsaI 1819 CCACGG C^C[GA][CT]GG
EagI 807 CGGCCG C^GGCCG
Eam1105I 16 GACAATCTGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
EclXI 807 CGGCCG C^GGCCG
Eco52I 807 CGGCCG C^GGCCG
Eco81I 2059 CCTCAGG CC^T[ACGT]AGG
EcoNI 2229 CCTGAAAGAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
Hin1I 1869 GACGCC G[GA]^CG[CT]C
MfeI 1412 CAATTG C^AATTG
MunI 1412 CAATTG C^AATTG
MvnI 98 CGCG CG^CG
NdeI 945 CATATG CA^TATG
PaeR7I 770 CTCGAG C^TCGAG
PshAI 2382 GACCTGCGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC
SapI 1527 GAAGAGC GCTCTTC(1/4)
ScaI 1226 AGTACT AGT^ACT
StuI 380 AGGCCT AGG^CCT
XhoI 770 CTCGAG C^TCGAG
XmnI 262 GAAGAAGTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
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# TWO CUT ENZYMES ( 33 enzymes) #
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AflIII 1576 ACGTGT A^C[GA][CT]GT
2085 ACGTGT
Alw44I 623 GTGCAC G^TGCAC
1194 GTGCAC
AlwNI 1418 CAGCAGCTG CAG[ACGT][ACGT][ACGT]^CTG
2496 CAGTAACTG
Aor51HI 1017 AGCGCT AGC^GCT
2258 AGCGCT
ApaLI 623 GTGCAC G^TGCAC
1194 GTGCAC
BalI 221 TGGCCA TGG^CCA
848 TGGCCA
BamHI 1052 GGATCC G^GATCC
1828 GGATCC
BbsI 13 GAAGAC GAAGAC(2/6)
1026 GAAGAC
BbvCI 1670 CCTCAGC CCTCAGC(-5/-2)
2119 GCTGAGG
BclI 818 TGATCA T^GATCA
1487 TGATCA
BcnI 464 CCCGG CC^[GC]GG
1857 CCCGG
BfaI 665 CTAG C^TAG
677 CTAG
BlpI 1263 GCTCAGC GC^T[ACGT]AGC
1810 GCTCAGC
Bpu1102I 1263 GCTCAGC GC^T[ACGT]AGC
1810 GCTCAGC
BpuAI 13 GAAGAC GAAGAC(2/6)
1026 GAAGAC
BspMI 1568 ACCTGC ACCTGC(4/8)
2383 ACCTGC
BstAPI 1270 GCAAAGCGTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
2345 GCAAGACGTGC
BstXI 554 CCAAGAGGGTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
728 CCATCTGTGTGG
CelII 1263 GCTCAGC GC^T[ACGT]AGC
1810 GCTCAGC
DraIII 914 CACAGAGTG CAC[ACGT][ACGT][ACGT]^GTG
977 CACAATGTG
Eco47III 1017 AGCGCT AGC^GCT
2258 AGCGCT
EcoRI 497 GAATTC G^AATTC
1310 GAATTC
FbaI 818 TGATCA T^GATCA
1487 TGATCA
HaeII 1017 AGCGCT [GA]GCGC^[CT]
2258 AGCGCT
HincII 282 GTCAAC GT[CT]^[GA]AC
507 GTCAAC
HindII 282 GTCAAC GT[CT]^[GA]AC
507 GTCAAC
MaeI 665 CTAG C^TAG
677 CTAG
MluNI 221 TGGCCA TGG^CCA
848 TGGCCA
MscI 221 TGGCCA TGG^CCA
848 TGGCCA
NciI 464 CCCGG CC^[GC]GG
1857 CCCGG
PvuII 1421 CAGCTG CAG^CTG
1778 CAGCTG
SacI 130 GAGCTC GAGCT^C
1755 GAGCTC
XcmI 851 CCAGAGAGAAGCTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
2409 CCACTGGCATGATGG
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# 3 OR MORE CUT ENZYMES ( 85 enzymes) #
#########################################
AciI AcsI AfaI AluI AlwI
ApoI AspHI AvaII BanII BbvI
BmyI BpmI BsaJI BseRI BsiHKAI
BsiYI BslI BsmAI BsmFI Bsp1286I
BsrI BssKI BstF5I BstNI BstYI
Cac8I CfoI Cfr13I DdeI DpnII
DraII EaeI EarI EcoO109I EcoRII
EcoT14I FauI Fnu4HI FokI HaeIII
HapII HgaI HhaI HinP1I HinfI
HpaII HphI ItaI Ksp632I MaeII
MaeIII MboI MboII MflI MnlI
MseI MslI MspA1I MspI MvaI
MwoI NdeII NlaIII NlaIV NspI
PleI PpuMI PstI RsaI Sau3AI
Sau96I ScrFI SfaNI SfcI SmlI
StyI TaqI TfiI Tru9I TseI
Tsp45I Tsp509I TspRI TthHB8I XhoII