Restriction map for
pFN21AE1695
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# NO CUT ENZYMES ( 136 enzymes) #
##################################
AatII Acc65I AccI AccII AccIII
AclI AcsI AflII AflIII AgeI
AhdI AlwNI AocI Aor51HI ApaI
ApoI AscI AseI AsnI Asp700I
Asp718I AspEI AviII AvrII BalI
BamHI BbsI BbvCI BciVI BclI
BfaI BfrI BlnI BpuAI BsaBI
BsaWI BseAI BsgI BsiWI BsmBI
BsmI Bsp1407I BspDI BspEI BspHI
BspLU11I BsrDI BsrGI BssHII BssSI
Bst1107I BstAPI BstBI BstEII BstPI
BstUI BstZ17I Bsu36I ClaI CpoI
DraI DraIII Eam1105I EarI Eco47III
Eco81I EcoO65I EcoRI EcoRV EcoT22I
FbaI FseI FspI HgaI HincII
HindII HindIII HpaI HphI KpnI
Ksp632I KspI MaeI MaeIII MamI
MfeI MluI MluNI MroI MscI
MseI MunI MvnI NdeI NheI
NotI NruI NsiI NspV PacI
PaeR7I PinAI PmeI PshAI PshBI
Psp1406I PvuI RcaI RsrII SacI
SacII SalI SapI ScaI SexAI
SfuI SgrAI SmaI SmlI SnaBI
SpeI SphI SspBI SspI StuI
SwaI TaqI TfiI Tru9I Tsp45I
TthHB8I XbaI XhoI XmaCI XmaI
XmnI
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# ONE CUT ENZYMES ( 58 enzymes) #
###################################
AcyI 352 GGCGCC G[GA]^CG[CT]C
Alw44I 367 GTGCAC G^TGCAC
AlwI 281 GGATC GGATC(4/5)
ApaLI 367 GTGCAC G^TGCAC
AspHI 367 GTGCAC G[AT]GC[AT]^C
AspI 475 GACTCGGTC GAC[ACGT]^[ACGT][ACGT]GTC
BanI 352 GGCGCC G^G[CT][GA]CC
BanII 168 GGGCTC G[GA]GC[CT]^C
BbeI 352 GGCGCC GGCGC^C
BbrPI 370 CACGTG CAC^GTG
BcgI 43 GCAGCTGGCTCG (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
BcnI 204 CCGGG CC^[GC]GG
BglII 533 AGATCT A^GATCT
BlpI 130 GCTCAGC GC^T[ACGT]AGC
BpmI 418 CTGGAG CTGGAG(16/14)
Bpu1102I 130 GCTCAGC GC^T[ACGT]AGC
BsaAI 370 CACGTG [CT]AC^GT[GA]
BsaHI 352 GGCGCC G[GA]^CG[CT]C
BsaI 385 GAGACC GGTCTC(1/5)
BseRI 600 GAGGAG GAGGAG(10/8)
BsiEI 327 CGGCCG CG[GA][CT]^CG
BsiHKAI 367 GTGCAC G[AT]GC[AT]^C
BspMI 291 GCAGGT ACCTGC(4/8)
BsrFI 80 GCCGGC [GA]^CCGG[CT]
BsrI 576 CCAGT ACTGG(1/-1)
BstXI 300 CCAGCTCTATGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
CelII 130 GCTCAGC GC^T[ACGT]AGC
Cfr10I 80 GCCGGC [GA]^CCGG[CT]
DrdI 313 GACACAGGGGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
DsaI 248 CCATGG C^C[GA][CT]GG
EaeI 327 CGGCCG [CT]^GGCC[GA]
EagI 327 CGGCCG C^GGCCG
EclXI 327 CGGCCG C^GGCCG
Eco52I 327 CGGCCG C^GGCCG
EcoNI 519 CCTGACTGAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
HaeII 352 GGCGCC [GA]GCGC^[CT]
Hin1I 352 GGCGCC G[GA]^CG[CT]C
KasI 352 GGCGCC G^GCGCC
MboII 536 TCTTC GAAGA(8/7)
MslI 243 CACAGCCATG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
NaeI 80 GCCGGC GCC^GGC
NarI 352 GGCGCC GG^CGCC
NciI 204 CCGGG CC^[GC]GG
NcoI 248 CCATGG C^CATGG
NgoMIV 80 GCCGGC G^CCGGC
NspI 225 GCATGT [GA]CATG^[CT]
PflFI 475 GACTCGGTC GAC[ACGT]^[ACGT][ACGT]GTC
PflMI 582 CCACGTCCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
PmaCI 370 CACGTG CAC^GTG
PmlI 370 CACGTG CAC^GTG
SbfI 186 CCTGCAGG CCTGCA^GG
SfiI 74 GGCCACGCCGGCC GGCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGCC
SfoI 352 GGCGCC GGC^GCC
Sse8387I 186 CCTGCAGG CCTGCA^GG
Tsp509I 121 AATT ^AATT
Tth111I 475 GACTCGGTC GAC[ACGT]^[ACGT][ACGT]GTC
Van91I 582 CCACGTCCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
XcmI 218 CCACACTGCATGTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
###################################
# TWO CUT ENZYMES ( 32 enzymes) #
###################################
AvaI 347 CCCGAG C^[CT]CG[GA]G
356 CCCGAG
BsmAI 311 GAGAC GTCTC(1/5)
385 GAGAC
BsmFI 13 GGGAC GGGAC(10/14)
145 GGGAC
BsoBI 347 CCCGAG C^[CT]CG[GA]G
356 CCCGAG
BsrBI 456 CCGCTC CCGCTC(-3/-3)
421 GAGCGG
BstYI 281 GGATCT [GA]^GATC[CT]
533 AGATCT
CfoI 297 GCGC GCG^C
353 GCGC
DpnII 282 GATC ^GATC
534 GATC
DraII 145 GGGACCT [GA]G^G[ACGT]CC[CT]
320 GGGTCCT
EcoO109I 145 GGGACCT [GA]G^G[ACGT]CC[CT]
320 GGGTCCT
EcoT14I 113 CCAAGG C^C[AT][AT]GG
248 CCATGG
HapII 81 CCGG C^CGG
204 CCGG
HhaI 297 GCGC GCG^C
353 GCGC
HinP1I 297 GCGC G^CGC
353 GCGC
HinfI 30 GACTC G^A[ACGT]TC
475 GACTC
HpaII 81 CCGG C^CGG
204 CCGG
MaeII 371 ACGT A^CGT
584 ACGT
MboI 282 GATC ^GATC
534 GATC
MflI 281 GGATCT [GA]^GATC[CT]
533 AGATCT
MspI 81 CCGG C^CGG
204 CCGG
NdeII 282 GATC ^GATC
534 GATC
NlaIII 226 CATG CATG^
249 CATG
PleI 30 GACTC GAGTC(4/5)
475 GACTC
PpuMI 145 GGGACCT [GA]G^G[AT]CC[CT]
320 GGGTCCT
PstI 187 CTGCAG CTGCA^G
569 CTGCAG
PvuII 44 CAGCTG CAG^CTG
433 CAGCTG
Sau3AI 282 GATC ^GATC
534 GATC
SfaNI 127 GATGC GCATC(5/9)
88 GCATC
SfcI 187 CTGCAG C^T[GA][CT]AG
569 CTGCAG
StyI 113 CCAAGG C^C[AT][AT]GG
248 CCATGG
TspRI 221 CACTG CA[GC]TG[ACGT][ACGT]^
440 CAGTG
XhoII 281 GGATCT [GA]^GATC[CT]
533 AGATCT
#########################################
# 3 OR MORE CUT ENZYMES ( 32 enzymes) #
#########################################
AciI AfaI AluI AvaII BbvI
BglI BmyI BsaJI BsiYI BslI
Bsp1286I BssKI BstF5I BstNI Cac8I
Cfr13I DdeI EcoRII FauI Fnu4HI
FokI HaeIII ItaI MnlI MspA1I
MvaI MwoI NlaIV RsaI Sau96I
ScrFI TseI