Restriction map for
pFN21AE3272
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# NO CUT ENZYMES ( 95 enzymes) #
##################################
Acc65I AccI AccIII AclI AflII
AgeI AhdI AocI AscI AseI
AsnI Asp700I Asp718I AspEI AspI
AviII AvrII BamHI BbvCI BciVI
BclI BfrI BlnI BsaBI BsaWI
BseAI BsiWI BsmBI BsmI Bsp1407I
BspEI BspHI BspLU11I BsrGI Bst1107I
BstBI BstXI BstZ17I Bsu36I CpoI
DraI DrdI Eam1105I EarI Eco81I
EcoRV EcoT22I FbaI FseI FspI
HindIII HpaI KpnI Ksp632I MamI
MfeI MluI MroI MseI MunI
NdeI NheI NotI NruI NsiI
NspV PacI PaeR7I PflFI PinAI
PmeI PshAI PshBI Psp1406I PvuI
RcaI RsrII SalI SapI SbfI
SexAI SfuI SgrAI SnaBI SpeI
SphI Sse8387I SspBI SspI SwaI
Tru9I Tth111I XbaI XhoI XmnI
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# ONE CUT ENZYMES ( 37 enzymes) #
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AatII 1429 GACGTC GACGT^C
AcsI 1648 GAATTC [GA]^AATT[CT]
Alw44I 1438 GTGCAC G^TGCAC
Aor51HI 1841 AGCGCT AGC^GCT
ApaLI 1438 GTGCAC G^TGCAC
ApoI 1648 GAATTC [GA]^AATT[CT]
BbrPI 1441 CACGTG CAC^GTG
BfaI 85 CTAG C^TAG
BglII 1070 AGATCT A^GATCT
BspDI 1831 ATCGAT AT^CGAT
BsrBI 1273 GAGCGG CCGCTC(-3/-3)
BssHII 918 GCGCGC G^CGCGC
BssSI 1452 CACGAG CACGAG(-5/-1)
BstAPI 1134 GCAGCTGGTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
BstEII 298 GGTGACC G^GT[ACGT]ACC
BstPI 298 GGTGACC G^GT[ACGT]ACC
ClaI 1831 ATCGAT AT^CGAT
DraIII 191 CACTGAGTG CAC[ACGT][ACGT][ACGT]^GTG
Eco47III 1841 AGCGCT AGC^GCT
EcoO65I 298 GGTGACC G^GT[ACGT]ACC
EcoRI 1648 GAATTC G^AATTC
HincII 1503 GTTGAC GT[CT]^[GA]AC
HindII 1503 GTTGAC GT[CT]^[GA]AC
KspI 20 CCGCGG CCGC^GG
MaeI 85 CTAG C^TAG
MaeIII 299 GTGAC ^GT[ACGT]AC
NspI 1802 ACATGC [GA]CATG^[CT]
PmaCI 1441 CACGTG CAC^GTG
PmlI 1441 CACGTG CAC^GTG
SacII 20 CCGCGG CCGC^GG
ScaI 809 AGTACT AGT^ACT
SfiI 1200 GGCCCAGGGGGCC GGCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGCC
SmlI 346 CTCAAG C^T[CT][GA]AG
TfiI 1730 GAATC G^A[AT]TC
Tsp45I 299 GTGAC ^GT[GC]AC
Tsp509I 1649 AATT ^AATT
XcmI 323 CCAGTGGAAACCTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
###################################
# TWO CUT ENZYMES ( 32 enzymes) #
###################################
AflIII 461 ACGTGT A^C[GA][CT]GT
1016 ACGTGT
BalI 707 TGGCCA TGG^CCA
1557 TGGCCA
BbeI 53 GGCGCC GGCGC^C
1028 GGCGCC
BcgI 366 CGACGAGGCTGC (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
828 GCAACTGTCTCG
BglI 209 GCCAGCTGGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
1201 GCCCAGGGGGC
BlpI 1233 GCTGAGC GC^T[ACGT]AGC
1924 GCTAAGC
Bpu1102I 1233 GCTGAGC GC^T[ACGT]AGC
1924 GCTAAGC
BsaAI 1015 TACGTG [CT]AC^GT[GA]
1441 CACGTG
BsaI 547 GAGACC GGTCTC(1/5)
1243 GAGACC
BsgI 163 GTGCAG GTGCAG(16/14)
1918 GTGCAG
BsrFI 1189 GCCGGC [GA]^CCGG[CT]
1682 GCCGGC
BstF5I 1458 CATCC GGATG(2/0)
68 GGATG
BstYI 1070 AGATCT [GA]^GATC[CT]
1950 GGATCT
CelII 1233 GCTGAGC GC^T[ACGT]AGC
1924 GCTAAGC
Cfr10I 1189 GCCGGC [GA]^CCGG[CT]
1682 GCCGGC
EcoNI 552 CCTGTGCAAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
591 CCTGGTGAAGG
FokI 1458 CATCC GGATG(9/13)
68 GGATG
KasI 53 GGCGCC G^GCGCC
1028 GGCGCC
MflI 1070 AGATCT [GA]^GATC[CT]
1950 GGATCT
MluNI 707 TGGCCA TGG^CCA
1557 TGGCCA
MscI 707 TGGCCA TGG^CCA
1557 TGGCCA
NaeI 1189 GCCGGC GCC^GGC
1682 GCCGGC
NarI 53 GGCGCC GG^CGCC
1028 GGCGCC
NcoI 1022 CCATGG C^CATGG
1095 CCATGG
NgoMIV 1189 GCCGGC G^CCGGC
1682 GCCGGC
PflMI 852 CCAGGCAGTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
957 CCAGGAGCTGG
PleI 1262 GACTC GAGTC(4/5)
1330 GACTC
SacI 31 GAGCTC GAGCT^C
1102 GAGCTC
SfoI 53 GGCGCC GGC^GCC
1028 GGCGCC
StuI 560 AGGCCT AGG^CCT
869 AGGCCT
Van91I 852 CCAGGCAGTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
957 CCAGGAGCTGG
XhoII 1070 AGATCT [GA]^GATC[CT]
1950 GGATCT
#########################################
# 3 OR MORE CUT ENZYMES ( 94 enzymes) #
#########################################
AccII AciI AcyI AfaI AluI
AlwI AlwNI ApaI AspHI AvaI
AvaII BanI BanII BbsI BbvI
BcnI BmyI BpmI BpuAI BsaHI
BsaJI BseRI BsiEI BsiHKAI BsiYI
BslI BsmAI BsmFI BsoBI Bsp1286I
BspMI BsrDI BsrI BssKI BstNI
BstUI Cac8I CfoI Cfr13I DdeI
DpnII DraII DsaI EaeI EagI
EclXI Eco52I EcoO109I EcoRII EcoT14I
FauI Fnu4HI HaeII HaeIII HapII
HgaI HhaI Hin1I HinP1I HinfI
HpaII HphI ItaI MaeII MboI
MboII MnlI MslI MspA1I MspI
MvaI MvnI MwoI NciI NdeII
NlaIII NlaIV PpuMI PstI PvuII
RsaI Sau3AI Sau96I ScrFI SfaNI
SfcI SmaI StyI TaqI TseI
TspRI TthHB8I XmaCI XmaI