Restriction map for
pFN21AB9719
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# NO CUT ENZYMES ( 120 enzymes) #
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AatII Acc65I AccIII AclI AcsI
AflII AflIII AgeI AhdI Alw44I
AocI ApaLI ApoI AseI AsnI
Asp700I Asp718I AspEI AspHI AspI
AviII AvrII BamHI BanI BbeI
BbsI BciVI BclI BfrI BglII
BlnI BlpI Bpu1102I BpuAI BsaBI
BsaWI BseAI BsiHKAI BsiWI BsmI
Bsp1407I BspDI BspEI BspHI BspLU11I
BsrBI BsrDI BsrGI Bst1107I BstBI
BstZ17I Bsu36I CelII ClaI CpoI
DraI DraIII Eam1105I Eco81I EcoRI
EcoRV EcoT22I FbaI FspI HincII
HindII HindIII HpaI KasI KpnI
MamI MfeI MluI MroI MseI
MunI NarI NcoI NdeI NruI
NsiI NspV PacI PaeR7I PflFI
PinAI PmeI PpuMI PshAI PshBI
Psp1406I PstI PvuI RcaI RsrII
SacI SalI SbfI ScaI SexAI
SfcI SfoI SfuI SgrAI SnaBI
SpeI SphI Sse8387I SspBI SspI
SwaI TaqI TfiI Tru9I Tsp509I
Tth111I TthHB8I XbaI XhoI XmnI
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# ONE CUT ENZYMES ( 54 enzymes) #
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AccI 1189 GTAGAC GT^[AC][GT]AC
AcyI 667 GACGCC G[GA]^CG[CT]C
AlwI 96 GATCC GGATC(4/5)
AlwNI 189 CAGCGACTG CAG[ACGT][ACGT][ACGT]^CTG
AscI 561 GGCGCGCC GG^CGCGCC
BalI 389 TGGCCA TGG^CCA
BbrPI 568 CACGTG CAC^GTG
BbvCI 772 GCTGAGG CCTCAGC(-5/-2)
BcgI 1152 CGACTTCCCTGC (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
BfaI 58 CTAG C^TAG
BsaAI 568 CACGTG [CT]AC^GT[GA]
BsaHI 667 GACGCC G[GA]^CG[CT]C
BsaI 777 GGTCTC GGTCTC(1/5)
BseRI 1012 CTCCTC GAGGAG(10/8)
BssSI 430 CTCGTG CACGAG(-5/-1)
BstEII 1144 GGTTACC G^GT[ACGT]ACC
BstPI 1144 GGTTACC G^GT[ACGT]ACC
BstYI 95 AGATCC [GA]^GATC[CT]
DpnII 96 GATC ^GATC
DraII 1075 GGGCCCC [GA]G^G[ACGT]CC[CT]
DrdI 769 GACGCTGAGGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
EcoNI 411 CCTCATGGAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
EcoO109I 1075 GGGCCCC [GA]G^G[ACGT]CC[CT]
EcoO65I 1144 GGTTACC G^GT[ACGT]ACC
FseI 865 GGCCGGCC GGCCGG^CC
Hin1I 667 GACGCC G[GA]^CG[CT]C
HinfI 34 GAGTC G^A[ACGT]TC
HphI 506 TCACC GGTGA(8/7)
KspI 717 CCGCGG CCGC^GG
MaeI 58 CTAG C^TAG
MaeII 569 ACGT A^CGT
MboI 96 GATC ^GATC
MflI 95 AGATCC [GA]^GATC[CT]
MluNI 389 TGGCCA TGG^CCA
MscI 389 TGGCCA TGG^CCA
NdeII 96 GATC ^GATC
NheI 57 GCTAGC G^CTAGC
NotI 489 GCGGCCGC GC^GGCCGC
NspI 605 ACATGC [GA]CATG^[CT]
PflMI 855 CCAGGCCTTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
PleI 34 GAGTC GAGTC(4/5)
PmaCI 568 CACGTG CAC^GTG
PmlI 568 CACGTG CAC^GTG
SacII 717 CCGCGG CCGC^GG
SapI 531 GAAGAGC GCTCTTC(1/4)
Sau3AI 96 GATC ^GATC
SfaNI 302 GCATC GCATC(5/9)
SfiI 910 GGCCTGGCAGGCC GGCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGCC
SmaI 29 CCCGGG CCC^GGG
Tsp45I 116 GTGAC ^GT[GC]AC
Van91I 855 CCAGGCCTTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
XhoII 95 AGATCC [GA]^GATC[CT]
XmaCI 29 CCCGGG C^CCGGG
XmaI 29 CCCGGG C^CCGGG
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# TWO CUT ENZYMES ( 15 enzymes) #
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BsiEI 490 CGGCCG CG[GA][CT]^CG
672 CGGCCG
BsmBI 523 GAGACG CGTCTC(1/5)
592 GAGACG
BssHII 7 GCGCGC G^CGCGC
562 GCGCGC
BstAPI 188 GCAGCGACTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
315 GCAGGTGGTGC
BstXI 311 CCACGCAGGTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
512 CCAAGCAGCTGG
DdeI 773 CTGAG C^T[ACGT]AG
1199 CTCAG
EagI 490 CGGCCG C^GGCCG
672 CGGCCG
EarI 531 GAAGAG CTCTTC(1/4)
651 GAAGAG
EclXI 490 CGGCCG C^GGCCG
672 CGGCCG
Eco52I 490 CGGCCG C^GGCCG
672 CGGCCG
Ksp632I 531 GAAGAG CTCTTC(1/4)
651 GAAGAG
MaeIII 116 GTGAC ^GT[ACGT]AC
1145 GTTAC
SmlI 145 CTCAAG C^T[CT][GA]AG
181 CTCAAG
StuI 857 AGGCCT AGG^CCT
909 AGGCCT
XcmI 851 CCACCCAGGCCTTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
1085 CCATGAGGGTGCTGG
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# 3 OR MORE CUT ENZYMES ( 69 enzymes) #
#########################################
AccII AciI AfaI AluI Aor51HI
ApaI AvaI AvaII BanII BbvI
BcnI BglI BmyI BpmI BsaJI
BsgI BsiYI BslI BsmAI BsmFI
BsoBI Bsp1286I BspMI BsrFI BsrI
BssKI BstF5I BstNI BstUI Cac8I
CfoI Cfr10I Cfr13I DsaI EaeI
Eco47III EcoRII EcoT14I FauI Fnu4HI
FokI HaeII HaeIII HapII HgaI
HhaI HinP1I HpaII ItaI MboII
MnlI MslI MspA1I MspI MvaI
MvnI MwoI NaeI NciI NgoMIV
NlaIII NlaIV PvuII RsaI Sau96I
ScrFI StyI TseI TspRI