Restriction map for
pFN21AE1796
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# NO CUT ENZYMES ( 163 enzymes) #
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AatII Acc65I AccIII AclI AcsI
AcyI AflII AflIII AgeI AhdI
Alw44I AlwI AlwNI Aor51HI ApaLI
ApoI AscI AseI AsnI Asp700I
Asp718I AspEI AspI AvaI AviII
AvrII BalI BamHI BbeI BbrPI
BbsI BbvCI BcgI BclI BfrI
BglII BlnI BlpI Bpu1102I BpuAI
BsaBI BsaHI BsaI BseAI BsgI
BsiEI BsiWI BsmAI BsmBI BsmI
BsoBI Bsp1407I BspDI BspEI BspHI
BspLU11I BsrDI BsrGI BssHII BssSI
Bst1107I BstAPI BstBI BstEII BstPI
BstXI BstYI BstZ17I CelII ClaI
CpoI DraI DraIII DrdI DsaI
EagI Eam1105I EarI EclXI Eco47III
Eco52I EcoNI EcoO65I EcoRI EcoRV
EcoT22I FbaI FseI FspI HgaI
Hin1I HincII HindII HindIII HpaI
KasI KpnI Ksp632I KspI MamI
MfeI MflI MluI MluNI MroI
MscI MslI MunI NarI NcoI
NdeI NheI NotI NruI NsiI
NspI NspV PacI PaeR7I PflFI
PflMI PinAI PmaCI PmeI PmlI
PshBI Psp1406I PstI PvuI PvuII
RcaI RsrII SacII SalI SapI
SbfI ScaI SfcI SfiI SfoI
SfuI SgrAI SmaI SmlI SnaBI
SpeI SphI Sse8387I SspBI SspI
StuI SwaI TaqI Tth111I TthHB8I
Van91I XbaI XcmI XhoI XhoII
XmaCI XmaI XmnI
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# ONE CUT ENZYMES ( 39 enzymes) #
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AccI 577 GTAGAC GT^[AC][GT]AC
AccII 305 CGCG CG^CG
AocI 837 CCTCAGG CC^T[ACGT]AGG
BanI 196 GGCACC G^G[CT][GA]CC
BciVI 892 GGATAC GTATCC(6/5)
BfaI 325 CTAG C^TAG
BsaAI 480 CACGTA [CT]AC^GT[GA]
BsaWI 760 ACCGGA [AT]^CCGG[AT]
BseRI 212 GAGGAG GAGGAG(10/8)
BsrBI 345 GAGCGG CCGCTC(-3/-3)
BsrFI 377 GCCGGC [GA]^CCGG[CT]
BstUI 305 CGCG CG^CG
Bsu36I 837 CCTCAGG CC^T[ACGT]AGG
CfoI 32 GCGC GCG^C
Cfr10I 377 GCCGGC [GA]^CCGG[CT]
EaeI 635 TGGCCG [CT]^GGCC[GA]
Eco81I 837 CCTCAGG CC^T[ACGT]AGG
EcoT14I 182 CCAAGG C^C[AT][AT]GG
HaeII 31 GGCGCT [GA]GCGC^[CT]
HhaI 32 GCGC GCG^C
HinP1I 32 GCGC G^CGC
MaeI 325 CTAG C^TAG
MaeIII 398 GTGAC ^GT[ACGT]AC
MboII 105 GAAGA GAAGA(8/7)
MseI 755 TTAA T^TAA
MspA1I 436 CAGCGG C[AC]G^C[GT]G
MvnI 305 CGCG CG^CG
NaeI 377 GCCGGC GCC^GGC
NgoMIV 377 GCCGGC G^CCGGC
PleI 846 GACTC GAGTC(4/5)
PshAI 88 GACACCTGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC
SacI 568 GAGCTC GAGCT^C
SexAI 581 ACCTGGT A^CC[AT]GGT
SfaNI 869 GCATC GCATC(5/9)
StyI 182 CCAAGG C^C[AT][AT]GG
TfiI 697 GATTC G^A[AT]TC
Tru9I 755 TTAA T^TAA
Tsp45I 398 GTGAC ^GT[GC]AC
TspRI 687 CAGTG CA[GC]TG[ACGT][ACGT]^
###################################
# TWO CUT ENZYMES ( 15 enzymes) #
###################################
BcnI 157 CCGGG CC^[GC]GG
468 CCGGG
BpmI 76 CTCCAG CTGGAG(16/14)
118 CTGGAG
BsmFI 470 GGGAC GGGAC(10/14)
844 GGGAC
BstF5I 537 CATCC GGATG(2/0)
447 GGATG
DpnII 623 GATC ^GATC
751 GATC
FauI 303 CCCGC CCCGC(4/6)
691 GCGGG
FokI 537 CATCC GGATG(9/13)
447 GGATG
HinfI 697 GATTC G^A[ACGT]TC
846 GACTC
HphI 102 GGTGA GGTGA(8/7)
137 TCACC
MboI 623 GATC ^GATC
751 GATC
NciI 157 CCGGG CC^[GC]GG
468 CCGGG
NdeII 623 GATC ^GATC
751 GATC
PpuMI 23 GGGTCCT [GA]G^G[AT]CC[CT]
317 AGGACCT
Sau3AI 623 GATC ^GATC
751 GATC
Tsp509I 766 AATT ^AATT
898 AATT
#########################################
# 3 OR MORE CUT ENZYMES ( 41 enzymes) #
#########################################
AciI AfaI AluI ApaI AspHI
AvaII BanII BbvI BglI BmyI
BsaJI BsiHKAI BsiYI BslI Bsp1286I
BspMI BsrI BssKI BstNI Cac8I
Cfr13I DdeI DraII EcoO109I EcoRII
Fnu4HI HaeIII HapII HpaII ItaI
MaeII MnlI MspI MvaI MwoI
NlaIII NlaIV RsaI Sau96I ScrFI
TseI