Restriction map for
pFN21AE5263
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# NO CUT ENZYMES ( 153 enzymes) #
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AatII AccI AccIII AclI AcsI
AflII AflIII AgeI AhdI Alw44I
AlwNI AocI Aor51HI ApaI ApaLI
ApoI AscI AseI AsnI AspEI
AspHI AspI AviII AvrII BalI
BanII BbrPI BbvCI BciVI BfaI
BfrI BglI BglII BlnI BlpI
BmyI Bpu1102I BsaAI BsaBI BsaWI
BseAI BsgI BsiHKAI BsiWI BsmBI
BsmFI Bsp1286I Bsp1407I BspDI BspEI
BspLU11I BspMI BsrBI BsrDI BsrGI
BssHII BssSI Bst1107I BstAPI BstBI
BstEII BstPI BstZ17I Bsu36I CelII
ClaI CpoI DraI DraII DraIII
DrdI DsaI Eam1105I Eco47III Eco81I
EcoO109I EcoO65I EcoRI EcoRV EcoT14I
EcoT22I FseI FspI HincII HindII
HindIII HpaI KspI MaeI MaeIII
MamI MfeI MluI MluNI MroI
MscI MseI MslI MunI NcoI
NdeI NheI NotI NruI NsiI
NspI NspV PacI PaeR7I PflFI
PflMI PinAI PmaCI PmeI PmlI
PpuMI PshAI PshBI Psp1406I PstI
PvuI PvuII RsrII SacI SacII
SalI SapI SbfI ScaI SexAI
SfcI SfiI SfuI SgrAI SmlI
SnaBI SpeI SphI Sse8387I SspBI
SspI StuI StyI SwaI TfiI
Tru9I Tsp45I Tsp509I Tth111I Van91I
XbaI XcmI XhoI
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# ONE CUT ENZYMES ( 47 enzymes) #
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Acc65I 77 GGTACC G^GTACC
Asp700I 198 GAATGCCTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
Asp718I 77 GGTACC G^GTACC
AvaI 22 CCCGGG C^[CT]CG[GA]G
AvaII 279 GGACC G^G[AT]CC
BamHI 452 GGATCC G^GATCC
BbsI 307 GTCTTC GAAGAC(2/6)
BcgI 371 GCAACGCCATCG (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
BclI 191 TGATCA T^GATCA
BpmI 502 CTGGAG CTGGAG(16/14)
BpuAI 307 GTCTTC GAAGAC(2/6)
BsaI 496 GAGACC GGTCTC(1/5)
BseRI 481 GAGGAG GAGGAG(10/8)
BsmI 198 GAATGC GAATGC(1/-1)
BsoBI 22 CCCGGG C^[CT]CG[GA]G
BspHI 194 TCATGA T^CATGA
BsrI 240 CCAGT ACTGG(1/-1)
BstXI 359 CCACGCTGCTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
BstYI 452 GGATCC [GA]^GATC[CT]
Cfr13I 279 GGACC G^G[ACGT]CC
DdeI 9 CTCAG C^T[ACGT]AG
EaeI 176 CGGCCG [CT]^GGCC[GA]
EagI 176 CGGCCG C^GGCCG
EclXI 176 CGGCCG C^GGCCG
Eco52I 176 CGGCCG C^GGCCG
EcoNI 443 CCTATGCCAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
FbaI 191 TGATCA T^GATCA
HgaI 28 GCGTC GACGC(5/10)
HinfI 407 GACTC G^A[ACGT]TC
HphI 152 TCACC GGTGA(8/7)
KpnI 77 GGTACC GGTAC^C
MaeII 70 ACGT A^CGT
MflI 452 GGATCC [GA]^GATC[CT]
MspA1I 81 CCGCTG C[AC]G^C[GT]G
NaeI 178 GCCGGC GCC^GGC
NgoMIV 178 GCCGGC G^CCGGC
PleI 407 GACTC GAGTC(4/5)
RcaI 194 TCATGA T^CATGA
Sau96I 279 GGACC G^G[ACGT]CC
SmaI 22 CCCGGG CCC^GGG
TaqI 248 TCGA T^CGA
TspRI 263 CAGTG CA[GC]TG[ACGT][ACGT]^
TthHB8I 248 TCGA T^CGA
XhoII 452 GGATCC [GA]^GATC[CT]
XmaCI 22 CCCGGG C^CCGGG
XmaI 22 CCCGGG C^CCGGG
XmnI 198 GAATGCCTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
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# TWO CUT ENZYMES ( 20 enzymes) #
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AfaI 78 GTAC GT^AC
402 GTAC
AlwI 453 GATCC GGATC(4/5)
452 GGATC
BbeI 18 GGCGCC GGCGC^C
225 GGCGCC
BcnI 22 CCCGG CC^[GC]GG
23 CCGGG
BsiEI 176 CGGCCG CG[GA][CT]^CG
326 CGGTCG
BsmAI 496 GAGAC GTCTC(1/5)
118 GTCTC
BsrFI 178 GCCGGC [GA]^CCGG[CT]
281 ACCGGC
Cfr10I 178 GCCGGC [GA]^CCGG[CT]
281 ACCGGC
EarI 139 CTCTTC CTCTTC(1/4)
58 GAAGAG
FauI 49 CCCGC CCCGC(4/6)
317 GCGGG
HaeII 18 GGCGCC [GA]GCGC^[CT]
225 GGCGCC
HaeIII 136 GGCC GG^CC
177 GGCC
KasI 18 GGCGCC G^GCGCC
225 GGCGCC
Ksp632I 139 CTCTTC CTCTTC(1/4)
58 GAAGAG
NarI 18 GGCGCC GG^CGCC
225 GGCGCC
NciI 22 CCCGG CC^[GC]GG
23 CCGGG
NlaIII 66 CATG CATG^
195 CATG
RsaI 78 GTAC GT^AC
402 GTAC
SfaNI 433 GATGC GCATC(5/9)
149 GCATC
SfoI 18 GGCGCC GGC^GCC
225 GGCGCC
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# 3 OR MORE CUT ENZYMES ( 38 enzymes) #
#########################################
AccII AciI AcyI AluI BanI
BbvI BsaHI BsaJI BsiYI BslI
BssKI BstF5I BstNI BstUI Cac8I
CfoI DpnII EcoRII Fnu4HI FokI
HapII HhaI Hin1I HinP1I HpaII
ItaI MboI MboII MnlI MspI
MvaI MvnI MwoI NdeII NlaIV
Sau3AI ScrFI TseI