Restriction map for
pFN21AE6742
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# NO CUT ENZYMES ( 70 enzymes) #
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AatII AccIII AclI AcsI AflII
AgeI ApoI BbvCI BciVI BclI
BfrI BglII BseAI BsiEI BsiWI
BsmI Bsp1407I BspDI BspEI BsrGI
Bst1107I BstBI BstZ17I ClaI CpoI
DraI EagI EclXI Eco52I EcoRI
EcoRV EcoT22I FbaI FseI HincII
HindII HindIII HpaI MfeI MluI
MroI MunI NdeI NheI NotI
NruI NsiI NspV PacI PaeR7I
PinAI PmeI Psp1406I PvuI RsrII
SacI SalI SbfI SfiI SfuI
SnaBI SpeI SphI Sse8387I SspBI
SspI SwaI Tsp509I XbaI XhoI
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# ONE CUT ENZYMES ( 34 enzymes) #
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Acc65I 4233 GGTACC G^GTACC
AccI 1747 GTCTAC GT^[AC][GT]AC
AhdI 4249 GACCCTCAGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
Alw44I 376 GTGCAC G^TGCAC
ApaLI 376 GTGCAC G^TGCAC
AscI 706 GGCGCGCC GG^CGCGCC
AseI 3985 ATTAAT AT^TAAT
AsnI 3985 ATTAAT AT^TAAT
Asp700I 337 GAACTGCTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
Asp718I 4233 GGTACC G^GTACC
AspEI 4249 GACCCTCAGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
AspI 517 GACCTGGTC GAC[ACGT]^[ACGT][ACGT]GTC
AviII 894 TGCGCA TGC^GCA
AvrII 3040 CCTAGG C^CTAGG
BcgI 1177 CGAAGTCCTTGC (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
BlnI 3040 CCTAGG C^CTAGG
BsaI 2485 GAGACC GGTCTC(1/5)
BsaWI 4236 ACCGGA [AT]^CCGG[AT]
BsmBI 2715 CGTCTC CGTCTC(1/5)
BspHI 1484 TCATGA T^CATGA
BssSI 3954 CACGAG CACGAG(-5/-1)
DrdI 649 GACCTGGGAGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
Eam1105I 4249 GACCCTCAGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
FspI 894 TGCGCA TGC^GCA
KpnI 4233 GGTACC GGTAC^C
PflFI 517 GACCTGGTC GAC[ACGT]^[ACGT][ACGT]GTC
PshAI 1740 GACCAGTGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC
PshBI 3985 ATTAAT AT^TAAT
RcaI 1484 TCATGA T^CATGA
ScaI 4211 AGTACT AGT^ACT
SgrAI 3280 CGCCGGCG C[GA]^CCGG[CT]G
TfiI 50 GAATC G^A[AT]TC
Tth111I 517 GACCTGGTC GAC[ACGT]^[ACGT][ACGT]GTC
XmnI 337 GAACTGCTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
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# TWO CUT ENZYMES ( 11 enzymes) #
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Aor51HI 302 AGCGCT AGC^GCT
3818 AGCGCT
BamHI 501 GGATCC G^GATCC
2918 GGATCC
BspLU11I 1454 ACATGT A^CATGT
3719 ACATGT
BssHII 707 GCGCGC G^CGCGC
835 GCGCGC
DraIII 379 CACCTGGTG CAC[ACGT][ACGT][ACGT]^GTG
2976 CACCCAGTG
Eco47III 302 AGCGCT AGC^GCT
3818 AGCGCT
KspI 856 CCGCGG CCGC^GG
875 CCGCGG
MseI 166 TTAA T^TAA
3986 TTAA
SacII 856 CCGCGG CCGC^GG
875 CCGCGG
SexAI 380 ACCTGGT A^CC[AT]GGT
518 ACCTGGT
Tru9I 166 TTAA T^TAA
3986 TTAA
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# 3 OR MORE CUT ENZYMES ( 143 enzymes) #
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AccII AciI AcyI AfaI AflIII
AluI AlwI AlwNI AocI ApaI
AspHI AvaI AvaII BalI BanI
BanII BbeI BbrPI BbsI BbvI
BcnI BfaI BglI BlpI BmyI
BpmI Bpu1102I BpuAI BsaAI BsaBI
BsaHI BsaJI BseRI BsgI BsiHKAI
BsiYI BslI BsmAI BsmFI BsoBI
Bsp1286I BspMI BsrBI BsrDI BsrFI
BsrI BssKI BstAPI BstEII BstF5I
BstNI BstPI BstUI BstXI BstYI
Bsu36I Cac8I CelII CfoI Cfr10I
Cfr13I DdeI DpnII DraII DsaI
EaeI EarI Eco81I EcoNI EcoO109I
EcoO65I EcoRII EcoT14I FauI Fnu4HI
FokI HaeII HaeIII HapII HgaI
HhaI Hin1I HinP1I HinfI HpaII
HphI ItaI KasI Ksp632I MaeI
MaeII MaeIII MamI MboI MboII
MflI MluNI MnlI MscI MslI
MspA1I MspI MvaI MvnI MwoI
NaeI NarI NciI NcoI NdeII
NgoMIV NlaIII NlaIV NspI PflMI
PleI PmaCI PmlI PpuMI PstI
PvuII RsaI SapI Sau3AI Sau96I
ScrFI SfaNI SfcI SfoI SmaI
SmlI StuI StyI TaqI TseI
Tsp45I TspRI TthHB8I Van91I XcmI
XhoII XmaCI XmaI