Restriction map for
pFN21AE5540
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# NO CUT ENZYMES ( 123 enzymes) #
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AatII Acc65I AccI AclI AfaI
AflII AflIII AgeI AhdI Alw44I
Aor51HI ApaLI AscI AseI AsnI
Asp700I Asp718I AspEI AspI AvrII
BamHI BanI BbeI BbrPI BcgI
BciVI BclI BfaI BfrI BlnI
BsaAI BsaBI BsaI BsiWI BsmAI
BsmBI BsmI Bsp1407I BspDI BspHI
BspLU11I BsrBI BsrDI BsrGI BsrI
BssSI Bst1107I BstBI BstEII BstPI
BstZ17I ClaI CpoI DraI DraIII
DrdI Eam1105I Eco47III EcoO65I EcoRI
EcoRV EcoT22I FbaI FseI HincII
HindII HindIII HpaI KasI KpnI
MaeI MaeII MamI MfeI MluI
MseI MslI MunI NarI NcoI
NdeI NheI NotI NruI NsiI
NspI NspV PacI PaeR7I PflFI
PinAI PmaCI PmeI PmlI PshAI
PshBI Psp1406I PvuI RcaI RsaI
RsrII SacI SalI SapI ScaI
SexAI SfoI SfuI SnaBI SpeI
SphI SspBI SspI SwaI TaqI
TfiI Tru9I Tth111I TthHB8I XbaI
XcmI XhoI XmnI
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# ONE CUT ENZYMES ( 46 enzymes) #
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AccIII 164 TCCGGA T^CCGGA
AcsI 160 AAATTC [GA]^AATT[CT]
AcyI 22 GACGCC G[GA]^CG[CT]C
AocI 247 CCTGAGG CC^T[ACGT]AGG
ApaI 124 GGGCCC GGGCC^C
ApoI 160 AAATTC [GA]^AATT[CT]
AspHI 190 GAGCAC G[AT]GC[AT]^C
AviII 986 TGCGCA TGC^GCA
BbsI 619 GAAGAC GAAGAC(2/6)
BbvCI 511 GCTGAGG CCTCAGC(-5/-2)
BglII 50 AGATCT A^GATCT
BpuAI 619 GAAGAC GAAGAC(2/6)
BsaHI 22 GACGCC G[GA]^CG[CT]C
BseAI 164 TCCGGA T^CCGGA
BsgI 564 CTGCAC GTGCAG(16/14)
BsiEI 932 CGGCCG CG[GA][CT]^CG
BsiHKAI 190 GAGCAC G[AT]GC[AT]^C
BspEI 164 TCCGGA T^CCGGA
BstAPI 978 GCAGAACCTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
BstXI 200 CCAAGTCCTTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
Bsu36I 247 CCTGAGG CC^T[ACGT]AGG
EagI 932 CGGCCG C^GGCCG
EclXI 932 CGGCCG C^GGCCG
Eco52I 932 CGGCCG C^GGCCG
Eco81I 247 CCTGAGG CC^T[ACGT]AGG
FspI 986 TGCGCA TGC^GCA
HgaI 22 GACGC GACGC(5/10)
Hin1I 22 GACGCC G[GA]^CG[CT]C
HinfI 325 GAGTC G^A[ACGT]TC
KspI 574 CCGCGG CCGC^GG
MaeIII 848 GTGAC ^GT[ACGT]AC
MroI 164 TCCGGA T^CCGGA
NlaIII 364 CATG CATG^
PflMI 531 CCAGGAGGTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
PleI 325 GAGTC GAGTC(4/5)
PpuMI 1058 AGGACCT [GA]G^G[AT]CC[CT]
PvuII 712 CAGCTG CAG^CTG
SacII 574 CCGCGG CCGC^GG
SbfI 726 CCTGCAGG CCTGCA^GG
SfiI 519 GGCCACGGAGGCC GGCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGCC
SgrAI 913 CGCCGGTG C[GA]^CCGG[CT]G
SmlI 104 CTCAAG C^T[CT][GA]AG
Sse8387I 726 CCTGCAGG CCTGCA^GG
Tsp45I 848 GTGAC ^GT[GC]AC
Tsp509I 161 AATT ^AATT
Van91I 531 CCAGGAGGTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
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# TWO CUT ENZYMES ( 23 enzymes) #
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AlwI 372 GATCC GGATC(4/5)
843 GATCC
AvaI 383 CCCGGG C^[CT]CG[GA]G
586 CCCGGG
BalI 41 TGGCCA TGG^CCA
311 TGGCCA
BanII 124 GGGCCC G[GA]GC[CT]^C
1028 GAGCCC
BlpI 187 GCTGAGC GC^T[ACGT]AGC
284 GCTCAGC
Bpu1102I 187 GCTGAGC GC^T[ACGT]AGC
284 GCTCAGC
BsaWI 164 TCCGGA [AT]^CCGG[AT]
458 ACCGGA
BseRI 817 CTCCTC GAGGAG(10/8)
514 GAGGAG
BsmFI 455 GGGAC GGGAC(10/14)
411 GTCCC
BsoBI 383 CCCGGG C^[CT]CG[GA]G
586 CCCGGG
BspMI 29 ACCTGC ACCTGC(4/8)
983 ACCTGC
BssHII 82 GCGCGC G^CGCGC
1068 GCGCGC
CelII 187 GCTGAGC GC^T[ACGT]AGC
284 GCTCAGC
DsaI 521 CCACGG C^C[GA][CT]GG
574 CCGCGG
HphI 612 GGTGA GGTGA(8/7)
669 GGTGA
MluNI 41 TGGCCA TGG^CCA
311 TGGCCA
MscI 41 TGGCCA TGG^CCA
311 TGGCCA
SfaNI 951 GATGC GCATC(5/9)
1046 GCATC
SmaI 383 CCCGGG CCC^GGG
586 CCCGGG
StuI 244 AGGCCT AGG^CCT
1074 AGGCCT
TspRI 315 CACTG CA[GC]TG[ACGT][ACGT]^
396 CAGTG
XmaCI 383 CCCGGG C^CCGGG
586 CCCGGG
XmaI 383 CCCGGG C^CCGGG
586 CCCGGG
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# 3 OR MORE CUT ENZYMES ( 66 enzymes) #
#########################################
AccII AciI AluI AlwNI AvaII
BbvI BcnI BglI BmyI BpmI
BsaJI BsiYI BslI Bsp1286I BsrFI
BssKI BstF5I BstNI BstUI BstYI
Cac8I CfoI Cfr10I Cfr13I DdeI
DpnII DraII EaeI EarI EcoNI
EcoO109I EcoRII EcoT14I FauI Fnu4HI
FokI HaeII HaeIII HapII HhaI
HinP1I HpaII ItaI Ksp632I MboI
MboII MflI MnlI MspA1I MspI
MvaI MvnI MwoI NaeI NciI
NdeII NgoMIV NlaIV PstI Sau3AI
Sau96I ScrFI SfcI StyI TseI
XhoII