Restriction map for
pFN21AE3211
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# NO CUT ENZYMES ( 115 enzymes) #
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AccI AccIII AclI AcsI AflII
AgeI AhdI Alw44I Aor51HI ApaI
ApaLI ApoI AscI Asp700I AspEI
AviII AvrII BbeI BbrPI BbvCI
BclI BcnI BfaI BfrI BglII
BlnI BsaAI BsaBI BsaI BsaWI
BseAI BsgI BsiEI BsiWI BsmI
BspDI BspEI BspLU11I BsrFI BssSI
Bst1107I BstEII BstPI BstXI BstZ17I
Cfr10I ClaI CpoI DraI DraIII
DrdI EagI Eam1105I EclXI Eco47III
Eco52I EcoO65I EcoRI EcoRV EcoT22I
FbaI FseI FspI HapII HincII
HindII HindIII HpaI HpaII KasI
MaeI MamI MfeI MroI MspI
MunI NaeI NarI NciI NdeI
NgoMIV NheI NotI NruI NsiI
PacI PaeR7I PinAI PmaCI PmeI
PmlI Psp1406I PvuI RsrII SalI
SbfI ScaI SexAI SfiI SfoI
SgrAI SmaI SmlI SnaBI SpeI
SphI Sse8387I StuI SwaI XbaI
XcmI XhoI XmaCI XmaI XmnI
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# ONE CUT ENZYMES ( 51 enzymes) #
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AatII 742 GACGTC GACGT^C
Acc65I 1202 GGTACC G^GTACC
AocI 200 CCTCAGG CC^T[ACGT]AGG
AseI 1411 ATTAAT AT^TAAT
AsnI 1411 ATTAAT AT^TAAT
Asp718I 1202 GGTACC G^GTACC
AspI 502 GACACGGTC GAC[ACGT]^[ACGT][ACGT]GTC
BalI 580 TGGCCA TGG^CCA
BamHI 449 GGATCC G^GATCC
BanII 943 GAGCTC G[GA]GC[CT]^C
BcgI 806 CGAGGGTGCTGC (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
BciVI 26 GTATCC GTATCC(6/5)
BglI 856 GCCCTCCTGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
BlpI 1005 GCTGAGC GC^T[ACGT]AGC
Bpu1102I 1005 GCTGAGC GC^T[ACGT]AGC
Bsp1407I 1214 TGTACA T^GTACA
BsrBI 1159 GAGCGG CCGCTC(-3/-3)
BsrDI 220 GCAATG GCAATG(2/0)
BsrGI 1214 TGTACA T^GTACA
BssHII 344 GCGCGC G^CGCGC
BstAPI 81 GCAGAACATGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
BstBI 1387 TTCGAA TT^CGAA
Bsu36I 200 CCTCAGG CC^T[ACGT]AGG
CelII 1005 GCTGAGC GC^T[ACGT]AGC
Eco81I 200 CCTCAGG CC^T[ACGT]AGG
EcoT14I 1232 CCATGG C^C[AT][AT]GG
HaeII 1013 AGCGCC [GA]GCGC^[CT]
KpnI 1202 GGTACC GGTAC^C
KspI 1066 CCGCGG CCGC^GG
MaeIII 1189 GTCAC ^GT[ACGT]AC
MluI 430 ACGCGT A^CGCGT
MluNI 580 TGGCCA TGG^CCA
MscI 580 TGGCCA TGG^CCA
MslI 996 CATGAAGGTG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
NcoI 1232 CCATGG C^CATGG
NspI 86 ACATGC [GA]CATG^[CT]
NspV 1387 TTCGAA TT^CGAA
PflFI 502 GACACGGTC GAC[ACGT]^[ACGT][ACGT]GTC
PleI 1167 GACTC GAGTC(4/5)
PshAI 762 GACCTATGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC
PshBI 1411 ATTAAT AT^TAAT
SacI 943 GAGCTC GAGCT^C
SacII 1066 CCGCGG CCGC^GG
SapI 1276 GAAGAGC GCTCTTC(1/4)
SfuI 1387 TTCGAA TT^CGAA
SspBI 1214 TGTACA T^GTACA
SspI 183 AATATT AAT^ATT
StyI 1232 CCATGG C^C[AT][AT]GG
Tsp45I 1189 GTCAC ^GT[GC]AC
Tsp509I 1179 AATT ^AATT
Tth111I 502 GACACGGTC GAC[ACGT]^[ACGT][ACGT]GTC
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# TWO CUT ENZYMES ( 16 enzymes) #
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AflIII 430 ACGCGT A^C[GA][CT]GT
477 ACACGT
AspHI 494 GTGCTC G[AT]GC[AT]^C
943 GAGCTC
BanI 615 GGTGCC G^G[CT][GA]CC
1202 GGTACC
BsiHKAI 494 GTGCTC G[AT]GC[AT]^C
943 GAGCTC
BspHI 134 TCATGA T^CATGA
995 TCATGA
BspMI 1088 ACCTGC ACCTGC(4/8)
1281 GCAGGT
DraII 1070 GGGACCT [GA]G^G[ACGT]CC[CT]
1370 AGGACCC
DsaI 1066 CCGCGG C^C[GA][CT]GG
1232 CCATGG
EcoNI 993 CCTCATGAAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
1074 CCTCGACCAGG
EcoO109I 1070 GGGACCT [GA]G^G[ACGT]CC[CT]
1370 AGGACCC
MseI 277 TTAA T^TAA
1412 TTAA
PpuMI 1070 GGGACCT [GA]G^G[AT]CC[CT]
1370 AGGACCC
PstI 847 CTGCAG CTGCA^G
1255 CTGCAG
PvuII 358 CAGCTG CAG^CTG
1319 CAGCTG
RcaI 134 TCATGA T^CATGA
995 TCATGA
Tru9I 277 TTAA T^TAA
1412 TTAA
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# 3 OR MORE CUT ENZYMES ( 76 enzymes) #
#########################################
AccII AciI AcyI AfaI AluI
AlwI AlwNI AvaI AvaII BbsI
BbvI BmyI BpmI BpuAI BsaHI
BsaJI BseRI BsiYI BslI BsmAI
BsmBI BsmFI BsoBI Bsp1286I BsrI
BssKI BstF5I BstNI BstUI BstYI
Cac8I CfoI Cfr13I DdeI DpnII
EaeI EarI EcoRII FauI Fnu4HI
FokI HaeIII HgaI HhaI Hin1I
HinP1I HinfI HphI ItaI Ksp632I
MaeII MboI MboII MflI MnlI
MspA1I MvaI MvnI MwoI NdeII
NlaIII NlaIV PflMI RsaI Sau3AI
Sau96I ScrFI SfaNI SfcI TaqI
TfiI TseI TspRI TthHB8I Van91I
XhoII