Restriction map for
pFN21AE6736
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# NO CUT ENZYMES ( 60 enzymes) #
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AccIII AclI AflII AgeI AscI
AseI AsnI Asp700I AviII BclI
BfrI BglII BsaBI BsaWI BseAI
BsiWI BspDI BspEI BspHI BspLU11I
BssHII Bst1107I BstBI BstZ17I ClaI
CpoI DraI DrdI EcoRI EcoRV
FbaI FspI HpaI MamI MfeI
MluI MroI MunI NdeI NheI
NotI NruI NspV PacI PinAI
PmeI PshBI Psp1406I PvuI RcaI
RsrII SalI ScaI SfuI SgrAI
SnaBI SpeI SspI SwaI XmnI
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# ONE CUT ENZYMES ( 35 enzymes) #
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AatII 3181 GACGTC GACGT^C
Acc65I 3629 GGTACC G^GTACC
AccI 3184 GTCTAC GT^[AC][GT]AC
AcsI 1107 GAATTT [GA]^AATT[CT]
Alw44I 2805 GTGCAC G^TGCAC
ApaLI 2805 GTGCAC G^TGCAC
ApoI 1107 GAATTT [GA]^AATT[CT]
Asp718I 3629 GGTACC G^GTACC
AvrII 369 CCTAGG C^CTAGG
BamHI 3319 GGATCC G^GATCC
BciVI 2068 GGATAC GTATCC(6/5)
BlnI 369 CCTAGG C^CTAGG
BsmBI 3410 CGTCTC CGTCTC(1/5)
BsmI 1003 GAATGC GAATGC(1/-1)
Bsp1407I 1716 TGTACA T^GTACA
BsrGI 1716 TGTACA T^GTACA
EcoT22I 2586 ATGCAT ATGCA^T
HincII 352 GTCAAC GT[CT]^[GA]AC
HindII 352 GTCAAC GT[CT]^[GA]AC
HindIII 4228 AAGCTT A^AGCTT
KpnI 3629 GGTACC GGTAC^C
MseI 1400 TTAA T^TAA
NsiI 2586 ATGCAT ATGCA^T
PaeR7I 3247 CTCGAG C^TCGAG
PflMI 3524 CCACTGCTTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
SapI 1023 GCTCTTC GCTCTTC(1/4)
SexAI 301 ACCTGGT A^CC[AT]GGT
SfiI 3465 GGCCACAGAGGCC GGCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGCC
SmlI 3247 CTCGAG C^T[CT][GA]AG
SspBI 1716 TGTACA T^GTACA
Tru9I 1400 TTAA T^TAA
Tsp509I 1108 AATT ^AATT
Van91I 3524 CCACTGCTTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
XbaI 711 TCTAGA T^CTAGA
XhoI 3247 CTCGAG C^TCGAG
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# TWO CUT ENZYMES ( 32 enzymes) #
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AflIII 155 ACACGT A^C[GA][CT]GT
725 ACACGT
AhdI 1150 GACTGCCAGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
2473 GACCCCCTGTC
AspEI 1150 GACTGCCAGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
2473 GACCCCCTGTC
AspI 4087 GACTCCGTC GAC[ACGT]^[ACGT][ACGT]GTC
4449 GACCTTGTC
BbeI 89 GGCGCC GGCGC^C
196 GGCGCC
BbrPI 156 CACGTG CAC^GTG
190 CACGTG
BcgI 4704 CGAGAACTGTGC (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
2225 GCAACCCTTTCG
BlpI 4369 GCTGAGC GC^T[ACGT]AGC
4389 GCTGAGC
Bpu1102I 4369 GCTGAGC GC^T[ACGT]AGC
4389 GCTGAGC
BsiEI 50 CGGCCG CG[GA][CT]^CG
513 CGGCCG
BstEII 684 GGTCACC G^GT[ACGT]ACC
2518 GGTGACC
BstPI 684 GGTCACC G^GT[ACGT]ACC
2518 GGTGACC
CelII 4369 GCTGAGC GC^T[ACGT]AGC
4389 GCTGAGC
DraIII 300 CACCTGGTG CAC[ACGT][ACGT][ACGT]^GTG
1085 CACACTGTG
EagI 50 CGGCCG C^GGCCG
513 CGGCCG
Eam1105I 1150 GACTGCCAGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
2473 GACCCCCTGTC
EarI 1024 CTCTTC CTCTTC(1/4)
3376 GAAGAG
EclXI 50 CGGCCG C^GGCCG
513 CGGCCG
Eco52I 50 CGGCCG C^GGCCG
513 CGGCCG
EcoO65I 684 GGTCACC G^GT[ACGT]ACC
2518 GGTGACC
FseI 514 GGCCGGCC GGCCGG^CC
634 GGCCGGCC
KasI 89 GGCGCC G^GCGCC
196 GGCGCC
Ksp632I 1024 CTCTTC CTCTTC(1/4)
3376 GAAGAG
NarI 89 GGCGCC GG^CGCC
196 GGCGCC
NcoI 320 CCATGG C^CATGG
466 CCATGG
PflFI 4087 GACTCCGTC GAC[ACGT]^[ACGT][ACGT]GTC
4449 GACCTTGTC
PmaCI 156 CACGTG CAC^GTG
190 CACGTG
PmlI 156 CACGTG CAC^GTG
190 CACGTG
SbfI 1824 CCTGCAGG CCTGCA^GG
2891 CCTGCAGG
SfoI 89 GGCGCC GGC^GCC
196 GGCGCC
Sse8387I 1824 CCTGCAGG CCTGCA^GG
2891 CCTGCAGG
Tth111I 4087 GACTCCGTC GAC[ACGT]^[ACGT][ACGT]GTC
4449 GACCTTGTC
#########################################
# 3 OR MORE CUT ENZYMES ( 131 enzymes) #
#########################################
AccII AciI AcyI AfaI AluI
AlwI AlwNI AocI Aor51HI ApaI
AspHI AvaI AvaII BalI BanI
BanII BbsI BbvCI BbvI BcnI
BfaI BglI BmyI BpmI BpuAI
BsaAI BsaHI BsaI BsaJI BseRI
BsgI BsiHKAI BsiYI BslI BsmAI
BsmFI BsoBI Bsp1286I BspMI BsrBI
BsrDI BsrFI BsrI BssKI BssSI
BstAPI BstF5I BstNI BstUI BstXI
BstYI Bsu36I Cac8I CfoI Cfr10I
Cfr13I DdeI DpnII DraII DsaI
EaeI Eco47III Eco81I EcoNI EcoO109I
EcoRII EcoT14I FauI Fnu4HI FokI
HaeII HaeIII HapII HgaI HhaI
Hin1I HinP1I HinfI HpaII HphI
ItaI KspI MaeI MaeII MaeIII
MboI MboII MflI MluNI MnlI
MscI MslI MspA1I MspI MvaI
MvnI MwoI NaeI NciI NdeII
NgoMIV NlaIII NlaIV NspI PleI
PpuMI PshAI PstI PvuII RsaI
SacI SacII Sau3AI Sau96I ScrFI
SfaNI SfcI SmaI SphI StuI
StyI TaqI TfiI TseI Tsp45I
TspRI TthHB8I XcmI XhoII XmaCI
XmaI