Restriction map for
pFN21AE3022
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# NO CUT ENZYMES ( 170 enzymes) #
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AatII Acc65I AccI AccII AccIII
AclI AcsI AfaI AflII AflIII
AgeI AhdI Alw44I AlwI AocI
Aor51HI ApaLI ApoI AscI AseI
AsnI Asp700I Asp718I AspEI AspI
AvaI AvaII AviII AvrII BamHI
BbrPI BcgI BciVI BclI BcnI
BfaI BfrI BglII BlnI BlpI
Bpu1102I BsaAI BsaBI BsaI BsaWI
BseAI BsgI BsiWI BsmAI BsmBI
BsmFI BsmI BsoBI Bsp1407I BspDI
BspEI BspHI BspLU11I BspMI BsrBI
BsrDI BsrGI BssHII BssSI Bst1107I
BstAPI BstBI BstEII BstF5I BstPI
BstUI BstXI BstYI BstZ17I Bsu36I
CelII ClaI CpoI DraI DrdI
EagI Eam1105I EclXI Eco47III Eco52I
Eco81I EcoO65I EcoRI EcoRV EcoT22I
FbaI FokI FseI FspI HincII
HindII HindIII HpaI HphI KpnI
KspI MaeI MaeII MaeIII MamI
MfeI MflI MluI MroI MseI
MslI MunI MvnI NaeI NciI
NdeI NgoMIV NheI NotI NruI
NsiI NspV PacI PaeR7I PflFI
PflMI PinAI PleI PmaCI PmeI
PmlI PpuMI PshAI PshBI Psp1406I
PvuI PvuII RcaI RsaI RsrII
SacII SalI SapI SbfI ScaI
SexAI SfaNI SfuI SgrAI SmaI
SmlI SnaBI SpeI Sse8387I SspBI
SspI SwaI TaqI Tru9I Tsp45I
Tsp509I Tth111I TthHB8I Van91I XbaI
XhoI XhoII XmaCI XmaI XmnI
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# ONE CUT ENZYMES ( 47 enzymes) #
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AlwNI 193 CAGGCCCTG CAG[ACGT][ACGT][ACGT]^CTG
ApaI 136 GGGCCC GGGCC^C
AspHI 352 GAGCTC G[AT]GC[AT]^C
BalI 128 TGGCCA TGG^CCA
BanI 46 GGCGCC G^G[CT][GA]CC
BbeI 46 GGCGCC GGCGC^C
BbsI 318 GAAGAC GAAGAC(2/6)
BbvCI 346 GCTGAGG CCTCAGC(-5/-2)
BglI 121 GCCGACCTGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
BpmI 107 CTGGAG CTGGAG(16/14)
BpuAI 318 GAAGAC GAAGAC(2/6)
BseRI 349 GAGGAG GAGGAG(10/8)
BsiEI 399 CGGTCG CG[GA][CT]^CG
BsiHKAI 352 GAGCTC G[AT]GC[AT]^C
BsrFI 292 ACCGGC [GA]^CCGG[CT]
BsrI 63 ACTGG ACTGG(1/-1)
CfoI 47 GCGC GCG^C
Cfr10I 292 ACCGGC [GA]^CCGG[CT]
DpnII 21 GATC ^GATC
DraIII 148 CACTCCGTG CAC[ACGT][ACGT][ACGT]^GTG
EaeI 128 TGGCCA [CT]^GGCC[GA]
EcoNI 342 CCTGGCTGAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
FauI 310 GCGGG CCCGC(4/6)
HaeII 46 GGCGCC [GA]GCGC^[CT]
HapII 293 CCGG C^CGG
HgaI 85 GACGC GACGC(5/10)
HhaI 47 GCGC GCG^C
HinP1I 47 GCGC G^CGC
HinfI 254 GATTC G^A[ACGT]TC
HpaII 293 CCGG C^CGG
KasI 46 GGCGCC G^GCGCC
MboI 21 GATC ^GATC
MluNI 128 TGGCCA TGG^CCA
MscI 128 TGGCCA TGG^CCA
MspI 293 CCGG C^CGG
NarI 46 GGCGCC GG^CGCC
NdeII 21 GATC ^GATC
NspI 240 GCATGC [GA]CATG^[CT]
SacI 352 GAGCTC GAGCT^C
Sau3AI 21 GATC ^GATC
SfiI 120 GGCCGACCTGGCC GGCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGCC
SfoI 46 GGCGCC GGC^GCC
SphI 240 GCATGC GCATG^C
StuI 7 AGGCCT AGG^CCT
TfiI 254 GATTC G^A[AT]TC
TspRI 282 CAGTG CA[GC]TG[ACGT][ACGT]^
XcmI 80 CCACAGACGCCATGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
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# TWO CUT ENZYMES ( 12 enzymes) #
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AcyI 46 GGCGCC G[GA]^CG[CT]C
85 GACGCC
BsaHI 46 GGCGCC G[GA]^CG[CT]C
85 GACGCC
DdeI 175 CTGAG C^T[ACGT]AG
347 CTGAG
DsaI 89 CCATGG C^C[GA][CT]GG
131 CCATGG
EarI 31 CTCTTC CTCTTC(1/4)
71 GAAGAG
EcoT14I 89 CCATGG C^C[AT][AT]GG
131 CCATGG
Hin1I 46 GGCGCC G[GA]^CG[CT]C
85 GACGCC
Ksp632I 31 CTCTTC CTCTTC(1/4)
71 GAAGAG
MspA1I 40 CCGCTG C[AC]G^C[GT]G
324 CAGCGG
NcoI 89 CCATGG C^CATGG
131 CCATGG
PstI 220 CTGCAG CTGCA^G
249 CTGCAG
StyI 89 CCATGG C^C[AT][AT]GG
131 CCATGG
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# 3 OR MORE CUT ENZYMES ( 29 enzymes) #
#########################################
AciI AluI BanII BbvI BmyI
BsaJI BsiYI BslI Bsp1286I BssKI
BstNI Cac8I Cfr13I DraII EcoO109I
EcoRII Fnu4HI HaeIII ItaI MboII
MnlI MvaI MwoI NlaIII NlaIV
Sau96I ScrFI SfcI TseI