Restriction map for
pFN21AE1637
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# NO CUT ENZYMES ( 167 enzymes) #
##################################
AatII Acc65I AccII AccIII AclI
AcsI AcyI AflII AflIII AgeI
AhdI Alw44I AlwNI Aor51HI ApaLI
ApoI AscI AseI AsnI Asp700I
Asp718I AspEI AspHI AspI AviII
AvrII BamHI BbeI BbrPI BbvCI
BcgI BciVI BclI BfaI BfrI
BglII BlnI BlpI BpmI Bpu1102I
BsaBI BsaHI BseAI BseRI BsgI
BsiEI BsiHKAI BsiWI BsmBI BsmFI
BsmI Bsp1407I BspDI BspEI BspHI
BspLU11I BspMI BsrDI BsrFI BsrGI
BssHII Bst1107I BstAPI BstBI BstEII
BstPI BstUI BstXI BstZ17I CelII
Cfr10I ClaI CpoI DraI DrdI
EagI Eam1105I EarI EclXI Eco47III
Eco52I EcoNI EcoO65I EcoRI EcoRV
EcoT22I FbaI FseI FspI HaeII
HgaI Hin1I HincII HindII HindIII
HinfI HpaI KasI KpnI Ksp632I
KspI MaeI MamI MfeI MluI
MroI MseI MspA1I MunI MvnI
NaeI NarI NdeI NgoMIV NheI
NotI NruI NsiI NspI NspV
PacI PaeR7I PflFI PinAI PleI
PmaCI PmeI PmlI PshAI PshBI
Psp1406I PvuI PvuII RcaI RsrII
SacI SacII SalI SapI SbfI
ScaI SexAI SfoI SfuI SgrAI
SmaI SmlI SnaBI SpeI SphI
Sse8387I SspBI SspI SwaI TaqI
TfiI Tru9I Tsp509I TspRI Tth111I
TthHB8I XbaI XcmI XhoI XmaCI
XmaI XmnI
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# ONE CUT ENZYMES ( 48 enzymes) #
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AccI 262 GTCTAC GT^[AC][GT]AC
AfaI 81 GTAC GT^AC
AlwI 429 GATCC GGATC(4/5)
AocI 204 CCTCAGG CC^T[ACGT]AGG
ApaI 209 GGGCCC GGGCC^C
AvaI 139 CCCGAG C^[CT]CG[GA]G
AvaII 277 GGTCC G^G[AT]CC
BalI 89 TGGCCA TGG^CCA
BanI 366 GGCACC G^G[CT][GA]CC
BanII 209 GGGCCC G[GA]GC[CT]^C
BbsI 313 GTCTTC GAAGAC(2/6)
BpuAI 313 GTCTTC GAAGAC(2/6)
BsaAI 82 TACGTG [CT]AC^GT[GA]
BsaI 229 GAGACC GGTCTC(1/5)
BsaWI 215 ACCGGA [AT]^CCGG[AT]
BsoBI 139 CCCGAG C^[CT]CG[GA]G
BsrBI 375 CCGCTC CCGCTC(-3/-3)
BsrI 168 CCAGT ACTGG(1/-1)
BssSI 319 CTCGTG CACGAG(-5/-1)
BstYI 428 AGATCC [GA]^GATC[CT]
Bsu36I 204 CCTCAGG CC^T[ACGT]AGG
CfoI 410 GCGC GCG^C
DpnII 429 GATC ^GATC
DraIII 337 CACACCGTG CAC[ACGT][ACGT][ACGT]^GTG
EaeI 89 TGGCCA [CT]^GGCC[GA]
Eco81I 204 CCTCAGG CC^T[ACGT]AGG
EcoT14I 416 CCATGG C^C[AT][AT]GG
HhaI 410 GCGC GCG^C
HinP1I 410 GCGC G^CGC
HphI 18 GGTGA GGTGA(8/7)
MaeIII 382 GTGAC ^GT[ACGT]AC
MboI 429 GATC ^GATC
MflI 428 AGATCC [GA]^GATC[CT]
MluNI 89 TGGCCA TGG^CCA
MscI 89 TGGCCA TGG^CCA
MslI 72 CATCAAGATG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
NcoI 416 CCATGG C^CATGG
NdeII 429 GATC ^GATC
PflMI 9 CCACAGCCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
PpuMI 276 GGGTCCT [GA]G^G[AT]CC[CT]
RsaI 81 GTAC GT^AC
Sau3AI 429 GATC ^GATC
SfiI 33 GGCCCTCCCGGCC GGCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGCC
StuI 125 AGGCCT AGG^CCT
StyI 416 CCATGG C^C[AT][AT]GG
Tsp45I 382 GTGAC ^GT[GC]AC
Van91I 9 CCACAGCCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
XhoII 428 AGATCC [GA]^GATC[CT]
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# TWO CUT ENZYMES ( 17 enzymes) #
###################################
AluI 359 AGCT AG^CT
401 AGCT
BcnI 30 CCCGG CC^[GC]GG
39 CCCGG
BglI 34 GCCCTCCCGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
412 GCCTCCATGGC
BmyI 209 GGGCCC G[AGT]GC[ACT]^C
365 GGGCAC
BsmAI 153 GAGAC GTCTC(1/5)
229 GAGAC
Bsp1286I 209 GGGCCC G[AGT]GC[ACT]^C
365 GGGCAC
DdeI 151 CTGAG C^T[ACGT]AG
205 CTCAG
DsaI 341 CCGTGG C^C[GA][CT]GG
416 CCATGG
FauI 446 CCCGC CCCGC(4/6)
363 GCGGG
MaeII 83 ACGT A^CGT
191 ACGT
MboII 120 GAAGA GAAGA(8/7)
314 TCTTC
MwoI 34 GCCCTCCCGGC GC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GC
412 GCCTCCATGGC
NciI 30 CCCGG CC^[GC]GG
39 CCCGG
NlaIII 225 CATG CATG^
417 CATG
PstI 54 CTGCAG CTGCA^G
424 CTGCAG
SfaNI 145 GATGC GCATC(5/9)
300 GATGC
SfcI 54 CTGCAG C^T[GA][CT]AG
424 CTGCAG
#########################################
# 3 OR MORE CUT ENZYMES ( 26 enzymes) #
#########################################
AciI BbvI BsaJI BsiYI BslI
BssKI BstF5I BstNI Cac8I Cfr13I
DraII EcoO109I EcoRII Fnu4HI FokI
HaeIII HapII HpaII ItaI MnlI
MspI MvaI NlaIV Sau96I ScrFI
TseI