Restriction map for
pFN21AE1790
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# NO CUT ENZYMES ( 122 enzymes) #
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AatII Acc65I AccI AccIII AclI
AcsI AflII AgeI AhdI AocI
ApaI ApoI AscI AseI AsnI
Asp700I Asp718I AspEI AspI AviII
AvrII BamHI BbsI BbvCI BciVI
BclI BfaI BfrI BglII BlnI
BlpI Bpu1102I BpuAI BsaBI BsaI
BsaWI BseAI BseRI Bsp1407I BspDI
BspEI BspHI BspLU11I BsrDI BsrGI
BssSI Bst1107I BstAPI BstBI BstYI
BstZ17I Bsu36I CelII ClaI CpoI
DraI DraIII EagI Eam1105I EclXI
Eco52I Eco81I EcoNI EcoRI EcoRV
EcoT22I FbaI FspI HincII HindII
HindIII HpaI KpnI MaeI MamI
MfeI MflI MroI MseI MunI
NdeI NheI NotI NruI NsiI
NspI NspV PacI PaeR7I PflFI
PflMI PinAI PmeI PshBI Psp1406I
PstI PvuI RcaI RsrII SalI
SapI SbfI ScaI SfcI SfiI
SfuI SgrAI SpeI SphI Sse8387I
SspBI SspI StuI SwaI TfiI
Tru9I Tth111I Van91I XbaI XhoI
XhoII XmnI
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# ONE CUT ENZYMES ( 65 enzymes) #
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AcyI 96 GGCGCC G[GA]^CG[CT]C
Alw44I 361 GTGCAC G^TGCAC
AlwI 699 GATCC GGATC(4/5)
AlwNI 614 CAGAGGCTG CAG[ACGT][ACGT][ACGT]^CTG
Aor51HI 782 AGCGCT AGC^GCT
ApaLI 361 GTGCAC G^TGCAC
AvaII 169 GGACC G^G[AT]CC
BalI 191 TGGCCA TGG^CCA
BanII 727 GAGCTC G[GA]GC[CT]^C
BbeI 96 GGCGCC GGCGC^C
BbrPI 664 CACGTG CAC^GTG
BcgI 401 GCAGTGGCTTCG (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
BglI 871 GCCATCACGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
BsaHI 96 GGCGCC G[GA]^CG[CT]C
BsgI 585 CTGCAC GTGCAG(16/14)
BsiWI 239 CGTACG C^GTACG
BsmAI 308 GTCTC GTCTC(1/5)
BsmBI 307 CGTCTC CGTCTC(1/5)
BsmI 519 GAATGC GAATGC(1/-1)
BspMI 712 GCAGGT ACCTGC(4/8)
BsrBI 811 GAGCGG CCGCTC(-3/-3)
BsrI 777 ACTGG ACTGG(1/-1)
BssHII 272 GCGCGC G^CGCGC
BstEII 418 GGTCACC G^GT[ACGT]ACC
BstPI 418 GGTCACC G^GT[ACGT]ACC
BstXI 770 CCATCCCACTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
DdeI 481 CTGAG C^T[ACGT]AG
DpnII 699 GATC ^GATC
DraII 168 GGGACCC [GA]G^G[ACGT]CC[CT]
DrdI 299 GACCCGAGCGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
EarI 598 CTCTTC CTCTTC(1/4)
Eco47III 782 AGCGCT AGC^GCT
EcoO109I 168 GGGACCC [GA]G^G[ACGT]CC[CT]
EcoO65I 418 GGTCACC G^GT[ACGT]ACC
EcoT14I 461 CCATGG C^C[AT][AT]GG
FseI 15 GGCCGGCC GGCCGG^CC
Hin1I 96 GGCGCC G[GA]^CG[CT]C
HphI 420 TCACC GGTGA(8/7)
KasI 96 GGCGCC G^GCGCC
Ksp632I 598 CTCTTC CTCTTC(1/4)
MaeIII 419 GTCAC ^GT[ACGT]AC
MboI 699 GATC ^GATC
MluI 365 ACGCGT A^CGCGT
MluNI 191 TGGCCA TGG^CCA
MscI 191 TGGCCA TGG^CCA
MslI 735 CACCTACGTG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
NarI 96 GGCGCC GG^CGCC
NcoI 461 CCATGG C^CATGG
NdeII 699 GATC ^GATC
NlaIII 462 CATG CATG^
PmaCI 664 CACGTG CAC^GTG
PmlI 664 CACGTG CAC^GTG
PpuMI 168 GGGACCC [GA]G^G[AT]CC[CT]
PshAI 412 GACCGCGGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC
PvuII 292 CAGCTG CAG^CTG
SacI 727 GAGCTC GAGCT^C
Sau3AI 699 GATC ^GATC
SexAI 644 ACCAGGT A^CC[AT]GGT
SfaNI 744 GATGC GCATC(5/9)
SfoI 96 GGCGCC GGC^GCC
SnaBI 634 TACGTA TAC^GTA
StyI 461 CCATGG C^C[AT][AT]GG
Tsp45I 419 GTCAC ^GT[GC]AC
Tsp509I 723 AATT ^AATT
XcmI 749 CCAACGCACCTGTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
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# TWO CUT ENZYMES ( 15 enzymes) #
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AflIII 365 ACGCGT A^C[GA][CT]GT
470 ACACGT
AspHI 361 GTGCAC G[AT]GC[AT]^C
727 GAGCTC
BsiHKAI 361 GTGCAC G[AT]GC[AT]^C
727 GAGCTC
BsmFI 102 GGGAC GGGAC(10/14)
168 GGGAC
EaeI 191 TGGCCA [CT]^GGCC[GA]
425 TGGCCG
HgaI 85 GACGC GACGC(5/10)
306 GCGTC
HinfI 235 GAGTC G^A[ACGT]TC
355 GACTC
MboII 599 TCTTC GAAGA(8/7)
686 TCTTC
NaeI 16 GCCGGC GCC^GGC
113 GCCGGC
NgoMIV 16 GCCGGC G^CCGGC
113 GCCGGC
PleI 355 GACTC GAGTC(4/5)
235 GAGTC
SmaI 163 CCCGGG CCC^GGG
180 CCCGGG
SmlI 223 CTCAAG C^T[CT][GA]AG
865 CTCAAG
XmaCI 163 CCCGGG C^CCGGG
180 CCCGGG
XmaI 163 CCCGGG C^CCGGG
180 CCCGGG
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# 3 OR MORE CUT ENZYMES ( 56 enzymes) #
#########################################
AccII AciI AfaI AluI AvaI
BanI BbvI BcnI BmyI BpmI
BsaAI BsaJI BsiEI BsiYI BslI
BsoBI Bsp1286I BsrFI BssKI BstF5I
BstNI BstUI Cac8I CfoI Cfr10I
Cfr13I DsaI EcoRII FauI Fnu4HI
FokI HaeII HaeIII HapII HhaI
HinP1I HpaII ItaI KspI MaeII
MnlI MspA1I MspI MvaI MvnI
MwoI NciI NlaIV RsaI SacII
Sau96I ScrFI TaqI TseI TspRI
TthHB8I