Restriction map for
pFN21AE3026
##################################
# NO CUT ENZYMES ( 178 enzymes) #
##################################
AatII Acc65I AccI AccII AclI
AcyI AflII AflIII AgeI AhdI
Alw44I Aor51HI ApaI ApaLI AscI
AseI AsnI Asp700I Asp718I AspEI
AspI AvaI AvaII AviII AvrII
BalI BamHI BanI BbeI BbrPI
BbsI BbvCI BcgI BciVI BclI
BcnI BfaI BfrI BglI BglII
BlnI BlpI Bpu1102I BpuAI BsaAI
BsaBI BsaHI BsaI BsgI BsiEI
BsiWI BsmBI BsmI BsoBI Bsp1407I
BspDI BspHI BspLU11I BsrDI BsrFI
BsrGI BssHII BssSI Bst1107I BstAPI
BstBI BstEII BstPI BstUI BstXI
BstYI BstZ17I CelII Cfr10I ClaI
CpoI DraI DraII DraIII DrdI
DsaI EagI Eam1105I EarI EclXI
Eco47III Eco52I EcoNI EcoO109I EcoO65I
EcoRV EcoT22I FauI FbaI FseI
FspI HaeII HgaI Hin1I HincII
HindII HindIII HpaI KasI KpnI
Ksp632I KspI MaeI MaeII MamI
MfeI MflI MluI MluNI MscI
MslI MspA1I MunI MvnI NaeI
NarI NciI NcoI NdeI NgoMIV
NheI NotI NruI NsiI NspI
NspV PacI PaeR7I PflFI PflMI
PinAI PmaCI PmeI PmlI PpuMI
PshAI PshBI Psp1406I PstI PvuI
PvuII RcaI RsrII SacII SalI
SapI SbfI SexAI SfcI SfiI
SfoI SfuI SgrAI SmaI SmlI
SnaBI SpeI SphI Sse8387I SspBI
SspI StuI SwaI TfiI Tth111I
Van91I XbaI XcmI XhoI XhoII
XmaCI XmaI XmnI
###################################
# ONE CUT ENZYMES ( 43 enzymes) #
###################################
AccIII 113 TCCGGA T^CCGGA
AlwI 131 GGATC GGATC(4/5)
AlwNI 266 CAGCCCCTG CAG[ACGT][ACGT][ACGT]^CTG
AocI 310 CCTCAGG CC^T[ACGT]AGG
AspHI 46 GAGCTC G[AT]GC[AT]^C
BanII 46 GAGCTC G[GA]GC[CT]^C
BmyI 46 GAGCTC G[AGT]GC[ACT]^C
BsaWI 113 TCCGGA [AT]^CCGG[AT]
BseAI 113 TCCGGA T^CCGGA
BsiHKAI 46 GAGCTC G[AT]GC[AT]^C
Bsp1286I 46 GAGCTC G[AGT]GC[ACT]^C
BspEI 113 TCCGGA T^CCGGA
BspMI 119 ACCTGC ACCTGC(4/8)
BsrBI 154 GAGCGG CCGCTC(-3/-3)
Bsu36I 310 CCTCAGG CC^T[ACGT]AGG
Cac8I 185 GCGTGC GC[ACGT]^[ACGT]GC
CfoI 7 GCGC GCG^C
Cfr13I 302 GGGCC G^G[ACGT]CC
DpnII 132 GATC ^GATC
Eco81I 310 CCTCAGG CC^T[ACGT]AGG
EcoRI 197 GAATTC G^AATTC
EcoT14I 245 CCTTGG C^C[AT][AT]GG
HapII 114 CCGG C^CGG
HhaI 7 GCGC GCG^C
HinP1I 7 GCGC G^CGC
HpaII 114 CCGG C^CGG
HphI 391 GGTGA GGTGA(8/7)
MaeIII 79 GTCAC ^GT[ACGT]AC
MboI 132 GATC ^GATC
MboII 138 GAAGA GAAGA(8/7)
MroI 113 TCCGGA T^CCGGA
MspI 114 CCGG C^CGG
NdeII 132 GATC ^GATC
NlaIII 418 CATG CATG^
SacI 46 GAGCTC GAGCT^C
Sau3AI 132 GATC ^GATC
Sau96I 302 GGGCC G^G[ACGT]CC
ScaI 424 AGTACT AGT^ACT
SfaNI 217 GATGC GCATC(5/9)
StyI 245 CCTTGG C^C[AT][AT]GG
TaqI 75 TCGA T^CGA
Tsp45I 79 GTCAC ^GT[GC]AC
TthHB8I 75 TCGA T^CGA
###################################
# TWO CUT ENZYMES ( 18 enzymes) #
###################################
AcsI 141 GAATTT [GA]^AATT[CT]
197 GAATTC
AfaI 242 GTAC GT^AC
425 GTAC
ApoI 141 GAATTT [GA]^AATT[CT]
197 GAATTC
BseRI 308 CTCCTC GAGGAG(10/8)
428 CTCCTC
BsiYI 359 CCACACTGAGG CC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GG
382 CCCACTCCAGG
BslI 359 CCACACTGAGG CC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GG
382 CCCACTCCAGG
BsmAI 179 GAGAC GTCTC(1/5)
378 GTCTC
BsrI 224 ACTGG ACTGG(1/-1)
234 ACTGG
DdeI 311 CTCAG C^T[ACGT]AG
364 CTGAG
EaeI 157 CGGCCA [CT]^GGCC[GA]
190 CGGCCA
HinfI 77 GAGTC G^A[ACGT]TC
376 GAGTC
MseI 65 TTAA T^TAA
212 TTAA
MwoI 147 GCTAAAAGAGC GC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GC
274 GCCCTTGCTGC
NlaIV 99 GGAACC GG[ACGT]^[ACGT]CC
116 GGAACC
PleI 77 GAGTC GAGTC(4/5)
376 GAGTC
RsaI 242 GTAC GT^AC
425 GTAC
Tru9I 65 TTAA T^TAA
212 TTAA
Tsp509I 142 AATT ^AATT
198 AATT
#########################################
# 3 OR MORE CUT ENZYMES ( 19 enzymes) #
#########################################
AciI AluI BbvI BpmI BsaJI
BsmFI BssKI BstF5I BstNI EcoRII
Fnu4HI FokI HaeIII ItaI MnlI
MvaI ScrFI TseI TspRI