Restriction map for
pFN21AE4253
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# NO CUT ENZYMES ( 82 enzymes) #
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AatII AccIII AclI AgeI AhdI
Aor51HI ApaI AscI AspEI AspI
AviII BbeI BlpI Bpu1102I BsaBI
BsaWI BseAI BsiEI BsiWI BsmBI
BspDI BspEI BspLU11I BsrFI BssHII
Bst1107I BstAPI BstBI BstZ17I CelII
Cfr10I ClaI CpoI DrdI EagI
Eam1105I EclXI Eco47III Eco52I EcoNI
FseI FspI HincII HindII HpaI
KasI KspI MamI MluI MroI
NaeI NarI NdeI NgoMIV NheI
NotI NruI NspV PaeR7I PflFI
PinAI PmeI Psp1406I PvuI RsrII
SacI SacII SalI SbfI ScaI
SfiI SfoI SfuI SgrAI SnaBI
SpeI SphI Sse8387I SwaI Tth111I
XbaI XhoI
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# ONE CUT ENZYMES ( 70 enzymes) #
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Acc65I 439 GGTACC G^GTACC
AccI 98 GTAGAC GT^[AC][GT]AC
AccII 1307 CGCG CG^CG
AcyI 1525 GGCGTC G[GA]^CG[CT]C
AflII 498 CTTAAG C^TTAAG
AflIII 399 ACGTGT A^C[GA][CT]GT
Alw44I 259 GTGCAC G^TGCAC
AlwNI 2405 CAGCAACTG CAG[ACGT][ACGT][ACGT]^CTG
AocI 2917 CCTGAGG CC^T[ACGT]AGG
ApaLI 259 GTGCAC G^TGCAC
Asp700I 541 GAAATTTTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
Asp718I 439 GGTACC G^GTACC
AvaI 2484 CCCGGG C^[CT]CG[GA]G
AvrII 90 CCTAGG C^CTAGG
BalI 29 TGGCCA TGG^CCA
BamHI 2870 GGATCC G^GATCC
BanI 439 GGTACC G^G[CT][GA]CC
BbrPI 2077 CACGTG CAC^GTG
BbvCI 1800 GCTGAGG CCTCAGC(-5/-2)
BcgI 2579 GCAGAAGCATCG (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
BclI 1838 TGATCA T^GATCA
BfrI 498 CTTAAG C^TTAAG
BlnI 90 CCTAGG C^CTAGG
BsaAI 2077 CACGTG [CT]AC^GT[GA]
BsaHI 1525 GGCGTC G[GA]^CG[CT]C
BsmI 219 GAATGC GAATGC(1/-1)
BsoBI 2484 CCCGGG C^[CT]CG[GA]G
Bsp1407I 2549 TGTACA T^GTACA
BspHI 1467 TCATGA T^CATGA
BsrBI 966 CCGCTC CCGCTC(-3/-3)
BsrDI 665 GCAATG GCAATG(2/0)
BsrGI 2549 TGTACA T^GTACA
BssSI 2572 CTCGTG CACGAG(-5/-1)
BstEII 1869 GGTCACC G^GT[ACGT]ACC
BstPI 1869 GGTCACC G^GT[ACGT]ACC
BstUI 1307 CGCG CG^CG
Bsu36I 2917 CCTGAGG CC^T[ACGT]AGG
DraI 699 TTTAAA TTT^AAA
DraIII 2074 CACCACGTG CAC[ACGT][ACGT][ACGT]^GTG
EaeI 29 TGGCCA [CT]^GGCC[GA]
Eco81I 2917 CCTGAGG CC^T[ACGT]AGG
EcoO65I 1869 GGTCACC G^GT[ACGT]ACC
EcoRV 901 GATATC GAT^ATC
EcoT22I 1594 ATGCAT ATGCA^T
FbaI 1838 TGATCA T^GATCA
HaeII 2778 AGCGCC [GA]GCGC^[CT]
Hin1I 1525 GGCGTC G[GA]^CG[CT]C
HindIII 1398 AAGCTT A^AGCTT
KpnI 439 GGTACC GGTAC^C
MfeI 2264 CAATTG C^AATTG
MluNI 29 TGGCCA TGG^CCA
MscI 29 TGGCCA TGG^CCA
MunI 2264 CAATTG C^AATTG
MvnI 1307 CGCG CG^CG
NsiI 1594 ATGCAT ATGCA^T
PacI 1135 TTAATTAA TTAAT^TAA
PflMI 1057 CCACTCTTTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
PmaCI 2077 CACGTG CAC^GTG
PmlI 2077 CACGTG CAC^GTG
PshAI 2762 GACCCAAGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC
RcaI 1467 TCATGA T^CATGA
SexAI 2339 ACCTGGT A^CC[AT]GGT
SmaI 2484 CCCGGG CCC^GGG
SspBI 2549 TGTACA T^GTACA
StuI 1499 AGGCCT AGG^CCT
Van91I 1057 CCACTCTTTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
XcmI 2300 CCACCGACTCCATGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
XmaCI 2484 CCCGGG C^CCGGG
XmaI 2484 CCCGGG C^CCGGG
XmnI 541 GAAATTTTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
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# TWO CUT ENZYMES ( 20 enzymes) #
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AseI 1134 ATTAAT AT^TAAT
1138 ATTAAT
AsnI 1134 ATTAAT AT^TAAT
1138 ATTAAT
BciVI 759 GGATAC GTATCC(6/5)
782 GGATAC
BglI 1324 GCCCGCCAGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
2822 GCCAAGGAGGC
BglII 1213 AGATCT A^GATCT
1937 AGATCT
BseRI 2471 CTCCTC GAGGAG(10/8)
1318 GAGGAG
BsgI 2071 CTGCAC GTGCAG(16/14)
1481 GTGCAG
BspMI 870 GCAGGT ACCTGC(4/8)
1718 GCAGGT
CfoI 1306 GCGC GCG^C
2779 GCGC
EcoRI 1108 GAATTC G^AATTC
1918 GAATTC
HgaI 1526 GCGTC GACGC(5/10)
2224 GCGTC
HhaI 1306 GCGC GCG^C
2779 GCGC
HinP1I 1306 GCGC G^CGC
2779 GCGC
NcoI 1958 CCATGG C^CATGG
2309 CCATGG
NspI 1253 ACATGC [GA]CATG^[CT]
1596 GCATGT
PpuMI 2722 AGGACCC [GA]G^G[AT]CC[CT]
2945 AGGACCC
PshBI 1134 ATTAAT AT^TAAT
1138 ATTAAT
SspI 302 AATATT AAT^ATT
1035 AATATT
TaqI 2022 TCGA T^CGA
2588 TCGA
TthHB8I 2022 TCGA T^CGA
2588 TCGA
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# 3 OR MORE CUT ENZYMES ( 86 enzymes) #
#########################################
AciI AcsI AfaI AluI AlwI
ApoI AspHI AvaII BanII BbsI
BbvI BcnI BfaI BmyI BpmI
BpuAI BsaI BsaJI BsiHKAI BsiYI
BslI BsmAI BsmFI Bsp1286I BsrI
BssKI BstF5I BstNI BstXI BstYI
Cac8I Cfr13I DdeI DpnII DraII
DsaI EarI EcoO109I EcoRII EcoT14I
FauI Fnu4HI FokI HaeIII HapII
HinfI HpaII HphI ItaI Ksp632I
MaeI MaeII MaeIII MboI MboII
MflI MnlI MseI MslI MspA1I
MspI MvaI MwoI NciI NdeII
NlaIII NlaIV PleI PstI PvuII
RsaI SapI Sau3AI Sau96I ScrFI
SfaNI SfcI SmlI StyI TfiI
Tru9I TseI Tsp45I Tsp509I TspRI
XhoII