Restriction map for
pFN21AB7862
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# NO CUT ENZYMES ( 148 enzymes) #
##################################
AatII Acc65I AccI AccIII AclI
AcyI AflII AgeI AhdI Alw44I
Aor51HI ApaI ApaLI AscI AseI
AsnI Asp718I AspEI AspHI AviII
AvrII BalI BanI BbeI BbrPI
BbsI BbvCI BcgI BciVI BfaI
BfrI BglI BlnI BlpI Bpu1102I
BpuAI BsaAI BsaBI BsaHI BseAI
BsgI BsiEI BsiHKAI BsiWI BsmBI
BspDI BspEI BspMI BsrBI BsrFI
BssHII BssSI Bst1107I BstAPI BstBI
BstEII BstNI BstPI BstXI BstZ17I
CelII Cfr10I ClaI CpoI DraII
DraIII DrdI EaeI EagI Eam1105I
EarI EclXI Eco47III Eco52I EcoNI
EcoO109I EcoO65I EcoRII EcoRV FauI
FseI FspI HgaI Hin1I HincII
HindII HpaI KasI KpnI Ksp632I
MaeI MaeII MamI MfeI MluI
MluNI MroI MscI MunI MvaI
NaeI NarI NgoMIV NheI NotI
NruI NspV PacI PaeR7I PflMI
PinAI PmaCI PmeI PmlI PpuMI
PshAI PshBI Psp1406I PstI PvuI
PvuII RsrII SacI SalI SapI
SbfI ScaI SexAI SfcI SfiI
SfoI SfuI SgrAI SmaI SnaBI
SpeI SphI Sse8387I SspI SwaI
TaqI TthHB8I Van91I XbaI XcmI
XhoI XmaCI XmaI
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# ONE CUT ENZYMES ( 45 enzymes) #
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AccII 129 CGCG CG^CG
AocI 405 CCTCAGG CC^T[ACGT]AGG
AspI 290 GACCCAGTC GAC[ACGT]^[ACGT][ACGT]GTC
AvaI 115 CCCGAG C^[CT]CG[GA]G
AvaII 48 GGACC G^G[AT]CC
BamHI 147 GGATCC G^GATCC
BanII 112 GAGCCC G[GA]GC[CT]^C
BbvI 610 GCAGC GCAGC(8/12)
BclI 573 TGATCA T^GATCA
BcnI 83 CCGGG CC^[GC]GG
BglII 1189 AGATCT A^GATCT
BmyI 112 GAGCCC G[AGT]GC[ACT]^C
BsmFI 1544 GTCCC GGGAC(10/14)
BsmI 1300 GAATGC GAATGC(1/-1)
BsoBI 115 CCCGAG C^[CT]CG[GA]G
Bsp1286I 112 GAGCCC G[AGT]GC[ACT]^C
BspHI 1884 TCATGA T^CATGA
BsrDI 1304 GCAATG GCAATG(2/0)
BssKI 83 CCGGG ^CC[ACGT]GG
BstUI 129 CGCG CG^CG
Bsu36I 405 CCTCAGG CC^T[ACGT]AGG
Cac8I 121 GCCAGC GC[ACGT]^[ACGT]GC
CfoI 125 GCGC GCG^C
Cfr13I 48 GGACC G^G[ACGT]CC
DraI 1576 TTTAAA TTT^AAA
Eco81I 405 CCTCAGG CC^T[ACGT]AGG
EcoT22I 1838 ATGCAT ATGCA^T
FbaI 573 TGATCA T^GATCA
HaeII 124 AGCGCC [GA]GCGC^[CT]
HhaI 125 GCGC GCG^C
HinP1I 125 GCGC G^CGC
KspI 128 CCGCGG CCGC^GG
MvnI 129 CGCG CG^CG
NciI 83 CCGGG CC^[GC]GG
NlaIV 147 GGATCC GG[ACGT]^[ACGT]CC
NsiI 1838 ATGCAT ATGCA^T
PflFI 290 GACCCAGTC GAC[ACGT]^[ACGT][ACGT]GTC
RcaI 1884 TCATGA T^CATGA
SacII 128 CCGCGG CCGC^GG
Sau96I 48 GGACC G^G[ACGT]CC
ScrFI 83 CCGGG CC^[ACGT]GG
StuI 1590 AGGCCT AGG^CCT
TfiI 24 GATTC G^A[AT]TC
TseI 610 GCAGC G^C[AT]GC
Tth111I 290 GACCCAGTC GAC[ACGT]^[ACGT][ACGT]GTC
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# TWO CUT ENZYMES ( 20 enzymes) #
###################################
AfaI 1012 GTAC GT^AC
1684 GTAC
AlwNI 22 CAGATTCTG CAG[ACGT][ACGT][ACGT]^CTG
453 CAGTGACTG
BsaI 40 GAGACC GGTCTC(1/5)
6 GGTCTC
BsaWI 50 ACCGGA [AT]^CCGG[AT]
1030 ACCGGA
BseRI 403 CTCCTC GAGGAG(10/8)
54 GAGGAG
Bsp1407I 1011 TGTACA T^GTACA
1683 TGTACA
BsrGI 1011 TGTACA T^GTACA
1683 TGTACA
BstYI 147 GGATCC [GA]^GATC[CT]
1189 AGATCT
EcoRI 1106 GAATTC G^AATTC
1358 GAATTC
EcoT14I 268 CCATGG C^C[AT][AT]GG
1071 CCATGG
HindIII 860 AAGCTT A^AGCTT
1403 AAGCTT
MflI 147 GGATCC [GA]^GATC[CT]
1189 AGATCT
MspA1I 12 CAGCGG C[AC]G^C[GT]G
128 CCGCGG
MwoI 121 GCCAGCGCCGC GC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GC
1741 GCCTTTAATGC
NcoI 268 CCATGG C^CATGG
1071 CCATGG
PleI 920 GACTC GAGTC(4/5)
532 GAGTC
RsaI 1012 GTAC GT^AC
1684 GTAC
SspBI 1011 TGTACA T^GTACA
1683 TGTACA
StyI 268 CCATGG C^C[AT][AT]GG
1071 CCATGG
XhoII 147 GGATCC [GA]^GATC[CT]
1189 AGATCT
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# 3 OR MORE CUT ENZYMES ( 45 enzymes) #
#########################################
AciI AcsI AflIII AluI AlwI
ApoI Asp700I BpmI BsaJI BsiYI
BslI BsmAI BspLU11I BsrI BstF5I
DdeI DpnII DsaI Fnu4HI FokI
HaeIII HapII HinfI HpaII HphI
ItaI MaeIII MboI MboII MnlI
MseI MslI MspI NdeI NdeII
NlaIII NspI Sau3AI SfaNI SmlI
Tru9I Tsp45I Tsp509I TspRI XmnI