Restriction map for
pFN21AE5445
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# NO CUT ENZYMES ( 153 enzymes) #
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AatII Acc65I AccI AccII AccIII
AclI AcyI AflII AgeI AhdI
AlwI AlwNI AocI Aor51HI ApaI
AscI AseI AsnI Asp718I AspEI
AspI AvaI AvaII AviII AvrII
BamHI BbeI BcgI BciVI BcnI
BfrI BglII BlnI BlpI BpmI
Bpu1102I BsaHI BsaWI BseAI BsgI
BsiEI BsiWI BsmBI BsoBI BspEI
BspLU11I BspMI BsrFI BssHII BssSI
Bst1107I BstAPI BstBI BstEII BstPI
BstUI BstYI BstZ17I Bsu36I CelII
CfoI Cfr10I Cfr13I CpoI DraI
DraII DrdI EagI Eam1105I EclXI
Eco47III Eco52I Eco81I EcoNI EcoO109I
EcoO65I EcoRI EcoRV EcoT22I FseI
FspI HaeII HapII HhaI Hin1I
HinP1I HincII HindII HindIII HpaI
HpaII KasI KpnI KspI MfeI
MflI MluI MroI MspI MunI
MvnI NaeI NarI NciI NgoMIV
NheI NotI NruI NsiI NspI
NspV PacI PaeR7I PflFI PflMI
PinAI PleI PmeI PpuMI PshAI
PshBI Psp1406I PvuI PvuII RsrII
SacI SacII SalI SapI Sau96I
SbfI ScaI SexAI SfiI SfoI
SfuI SgrAI SmaI SmlI SnaBI
SpeI SphI Sse8387I SspI StuI
SwaI Tth111I Van91I XbaI XhoI
XhoII XmaCI XmaI
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# ONE CUT ENZYMES ( 62 enzymes) #
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AcsI 536 AAATTC [GA]^AATT[CT]
AflIII 529 ACGTGT A^C[GA][CT]GT
AluI 558 AGCT AG^CT
Alw44I 422 GTGCAC G^TGCAC
ApaLI 422 GTGCAC G^TGCAC
ApoI 536 AAATTC [GA]^AATT[CT]
Asp700I 70 GAAGTGGTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
BalI 371 TGGCCA TGG^CCA
BanI 721 GGTGCC G^G[CT][GA]CC
BanII 321 GGGCTC G[GA]GC[CT]^C
BbrPI 528 CACGTG CAC^GTG
BbsI 85 GTCTTC GAAGAC(2/6)
BbvCI 683 CCTCAGC CCTCAGC(-5/-2)
BbvI 757 GCTGC GCAGC(8/12)
BclI 140 TGATCA T^GATCA
BfaI 151 CTAG C^TAG
BglI 346 GCCGTGATGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
BpuAI 85 GTCTTC GAAGAC(2/6)
BsaAI 528 CACGTG [CT]AC^GT[GA]
BsaBI 141 GATCACTATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
BsaI 438 GGTCTC GGTCTC(1/5)
BseRI 610 CTCCTC GAGGAG(10/8)
BsmAI 439 GTCTC GTCTC(1/5)
BsmFI 309 GTCCC GGGAC(10/14)
BsmI 605 GCATTC GAATGC(1/-1)
Bsp1407I 294 TGTACA T^GTACA
BspDI 517 ATCGAT AT^CGAT
BsrBI 464 CCGCTC CCGCTC(-3/-3)
BsrDI 923 CATTGC GCAATG(2/0)
BsrGI 294 TGTACA T^GTACA
BstXI 587 CCATCATGCTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
Cac8I 559 GCTTGC GC[ACGT]^[ACGT]GC
ClaI 517 ATCGAT AT^CGAT
DraIII 327 CACAGAGTG CAC[ACGT][ACGT][ACGT]^GTG
DsaI 317 CCATGG C^C[GA][CT]GG
EarI 79 CTCTTC CTCTTC(1/4)
FauI 384 CCCGC CCCGC(4/6)
FbaI 140 TGATCA T^GATCA
Fnu4HI 757 GCTGC GC^[ACGT]GC
HgaI 416 GCGTC GACGC(5/10)
ItaI 757 GCTGC GC^[ACGT]GC
Ksp632I 79 CTCTTC CTCTTC(1/4)
MaeI 151 CTAG C^TAG
MaeII 529 ACGT A^CGT
MamI 141 GATCACTATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
MluNI 371 TGGCCA TGG^CCA
MscI 371 TGGCCA TGG^CCA
MspA1I 385 CCGCTG C[AC]G^C[GT]G
NcoI 317 CCATGG C^CATGG
NdeI 356 CATATG CA^TATG
PmaCI 528 CACGTG CAC^GTG
PmlI 528 CACGTG CAC^GTG
PstI 490 CTGCAG CTGCA^G
SfcI 490 CTGCAG C^T[GA][CT]AG
SspBI 294 TGTACA T^GTACA
TaqI 518 TCGA T^CGA
TseI 757 GCTGC G^C[AT]GC
Tsp45I 448 GTGAC ^GT[GC]AC
TspRI 572 CACTG CA[GC]TG[ACGT][ACGT]^
TthHB8I 518 TCGA T^CGA
XcmI 587 CCATCATGCTGGTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
XmnI 70 GAAGTGGTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
###################################
# TWO CUT ENZYMES ( 17 enzymes) #
###################################
AspHI 337 GTGCTC G[AT]GC[AT]^C
422 GTGCAC
BsiHKAI 337 GTGCTC G[AT]GC[AT]^C
422 GTGCAC
BspHI 404 TCATGA T^CATGA
578 TCATGA
BsrI 54 CCAGT ACTGG(1/-1)
479 CCAGT
DpnII 141 GATC ^GATC
520 GATC
EaeI 344 TGGCCG [CT]^GGCC[GA]
371 TGGCCA
EcoT14I 186 CCTTGG C^C[AT][AT]GG
317 CCATGG
HaeIII 345 GGCC GG^CC
372 GGCC
MboI 141 GATC ^GATC
520 GATC
MseI 164 TTAA T^TAA
871 TTAA
MwoI 346 GCCGTGATGGC GC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GC
424 GCACGGATGGC
NdeII 141 GATC ^GATC
520 GATC
NlaIV 322 GGCTCC GG[ACGT]^[ACGT]CC
721 GGTGCC
RcaI 404 TCATGA T^CATGA
578 TCATGA
Sau3AI 141 GATC ^GATC
520 GATC
StyI 186 CCTTGG C^C[AT][AT]GG
317 CCATGG
Tru9I 164 TTAA T^TAA
871 TTAA
#########################################
# 3 OR MORE CUT ENZYMES ( 26 enzymes) #
#########################################
AciI AfaI BmyI BsaJI BsiYI
BslI Bsp1286I BssKI BstF5I BstNI
DdeI EcoRII FokI HinfI HphI
MaeIII MboII MnlI MslI MvaI
NlaIII RsaI ScrFI SfaNI TfiI
Tsp509I