Restriction map for
pFN21AE5814
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# NO CUT ENZYMES ( 97 enzymes) #
##################################
Acc65I AccI AclI AlwNI Aor51HI
AscI Asp718I AspI AviII AvrII
BanI BbeI BbrPI BbsI BbvCI
BcgI BciVI BglI BglII BlnI
BlpI Bpu1102I BpuAI BsiEI BsiWI
BsmBI Bsp1407I BspDI BspLU11I BsrDI
BsrGI BssSI Bst1107I BstAPI BstBI
BstXI BstZ17I CelII ClaI CpoI
EagI EclXI Eco47III Eco52I EcoNI
EcoRI EcoRV EcoT22I FseI FspI
HaeII HindIII KasI KpnI MfeI
MluI MunI NaeI NarI NdeI
NgoMIV NheI NotI NruI NsiI
NspV PacI PaeR7I PflFI PmaCI
PmeI PmlI PpuMI PshAI Psp1406I
PvuI PvuII RsrII SacI SalI
SapI SbfI SexAI SfiI SfoI
SfuI SgrAI SnaBI SpeI SphI
Sse8387I SspBI StuI SwaI Tth111I
XbaI XhoI
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# ONE CUT ENZYMES ( 39 enzymes) #
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AatII 2287 GACGTC GACGT^C
AccIII 1869 TCCGGA T^CCGGA
AflII 1573 CTTAAG C^TTAAG
AflIII 1241 ACACGT A^C[GA][CT]GT
AgeI 2545 ACCGGT A^CCGGT
AocI 943 CCTCAGG CC^T[ACGT]AGG
ApaI 2155 GGGCCC GGGCC^C
BfrI 1573 CTTAAG C^TTAAG
BseAI 1869 TCCGGA T^CCGGA
BsmI 1646 GCATTC GAATGC(1/-1)
BspEI 1869 TCCGGA T^CCGGA
BspHI 2291 TCATGA T^CATGA
BsrBI 1797 CCGCTC CCGCTC(-3/-3)
BsrFI 2545 ACCGGT [GA]^CCGG[CT]
BssHII 2640 GCGCGC G^CGCGC
BstEII 1449 GGTGACC G^GT[ACGT]ACC
BstPI 1449 GGTGACC G^GT[ACGT]ACC
Bsu36I 943 CCTCAGG CC^T[ACGT]AGG
Cfr10I 2545 ACCGGT [GA]^CCGG[CT]
DraI 127 TTTAAA TTT^AAA
DrdI 1350 GACCCAGAAGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
EarI 1179 CTCTTC CTCTTC(1/4)
Eco81I 943 CCTCAGG CC^T[ACGT]AGG
EcoO65I 1449 GGTGACC G^GT[ACGT]ACC
HpaI 414 GTTAAC GTT^AAC
Ksp632I 1179 CTCTTC CTCTTC(1/4)
KspI 713 CCGCGG CCGC^GG
MroI 1869 TCCGGA T^CCGGA
MspA1I 713 CCGCGG C[AC]G^C[GT]G
NcoI 1898 CCATGG C^CATGG
NspI 860 ACATGC [GA]CATG^[CT]
PflMI 2721 CCAAATGCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
PinAI 2545 ACCGGT A^CCGGT
RcaI 2291 TCATGA T^CATGA
SacII 713 CCGCGG CCGC^GG
SmaI 2054 CCCGGG CCC^GGG
Van91I 2721 CCAAATGCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
XmaCI 2054 CCCGGG C^CCGGG
XmaI 2054 CCCGGG C^CCGGG
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# TWO CUT ENZYMES ( 29 enzymes) #
###################################
AcyI 1474 GACGCC G[GA]^CG[CT]C
2287 GACGTC
AhdI 1810 GACTTCAAGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
2253 GACCAAATGTC
Alw44I 172 GTGCAC G^TGCAC
2174 GTGCAC
ApaLI 172 GTGCAC G^TGCAC
2174 GTGCAC
AseI 34 ATTAAT AT^TAAT
2086 ATTAAT
AsnI 34 ATTAAT AT^TAAT
2086 ATTAAT
AspEI 1810 GACTTCAAGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
2253 GACCAAATGTC
AvaI 2054 CCCGGG C^[CT]CG[GA]G
2354 CCCGAG
AvaII 635 GGACC G^G[AT]CC
2252 GGACC
BamHI 1894 GGATCC G^GATCC
2730 GGATCC
BclI 1419 TGATCA T^GATCA
1778 TGATCA
BpmI 1391 CTCCAG CTGGAG(16/14)
2410 CTCCAG
BsaHI 1474 GACGCC G[GA]^CG[CT]C
2287 GACGTC
BsaI 2633 GAGACC GGTCTC(1/5)
2486 GGTCTC
BsaWI 1869 TCCGGA [AT]^CCGG[AT]
2545 ACCGGT
BsoBI 2054 CCCGGG C^[CT]CG[GA]G
2354 CCCGAG
Eam1105I 1810 GACTTCAAGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
2253 GACCAAATGTC
EcoT14I 760 CCTTGG C^C[AT][AT]GG
1898 CCATGG
FbaI 1419 TGATCA T^GATCA
1778 TGATCA
HgaI 1474 GACGC GACGC(5/10)
448 GCGTC
Hin1I 1474 GACGCC G[GA]^CG[CT]C
2287 GACGTC
HincII 414 GTTAAC GT[CT]^[GA]AC
2226 GTTGAC
HindII 414 GTTAAC GT[CT]^[GA]AC
2226 GTTGAC
PshBI 34 ATTAAT AT^TAAT
2086 ATTAAT
SfaNI 1596 GATGC GCATC(5/9)
95 GCATC
SmlI 1265 CTCAAG C^T[CT][GA]AG
1573 CTTAAG
SspI 203 AATATT AAT^ATT
1016 AATATT
StyI 760 CCTTGG C^C[AT][AT]GG
1898 CCATGG
XcmI 99 CCACTTTTTAGATGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
2600 CCATTTACCCACTGG
#########################################
# 3 OR MORE CUT ENZYMES ( 93 enzymes) #
#########################################
AccII AciI AcsI AfaI AluI
AlwI ApoI Asp700I AspHI BalI
BanII BbvI BcnI BfaI BmyI
BsaAI BsaBI BsaJI BseRI BsgI
BsiHKAI BsiYI BslI BsmAI BsmFI
Bsp1286I BspMI BsrI BssKI BstF5I
BstNI BstUI BstYI Cac8I CfoI
Cfr13I DdeI DpnII DraII DraIII
DsaI EaeI EcoO109I EcoRII FauI
Fnu4HI FokI HaeIII HapII HhaI
HinP1I HinfI HpaII HphI ItaI
MaeI MaeII MaeIII MamI MboI
MboII MflI MluNI MnlI MscI
MseI MslI MspI MvaI MvnI
MwoI NciI NdeII NlaIII NlaIV
PleI PstI RsaI Sau3AI Sau96I
ScaI ScrFI SfcI TaqI TfiI
Tru9I TseI Tsp45I Tsp509I TspRI
TthHB8I XhoII XmnI