Restriction map for
pFN21AE1943
##################################
# NO CUT ENZYMES ( 137 enzymes) #
##################################
AatII Acc65I AccIII AclI AcyI
AflII AgeI AhdI Alw44I AocI
Aor51HI ApaI ApaLI AscI Asp700I
Asp718I AspEI AspHI AspI AvaI
AviII AvrII BalI BamHI BanII
BbeI BbrPI BciVI BfrI BglI
BlnI BlpI BpmI Bpu1102I BsaAI
BsaBI BsaHI BsaWI BseAI BseRI
BsiHKAI BsiWI BsmBI BsmI BsoBI
BspDI BspEI BspLU11I BspMI BsrFI
BssHII BssSI Bst1107I BstBI BstXI
BstZ17I Bsu36I CelII Cfr10I ClaI
CpoI DrdI DsaI EaeI EagI
Eam1105I EarI EclXI Eco47III Eco52I
Eco81I EcoNI EcoT22I FseI FspI
HgaI Hin1I HindIII HpaI KasI
KpnI Ksp632I KspI MamI MfeI
MluI MluNI MroI MscI MspA1I
MunI NaeI NarI NcoI NdeI
NgoMIV NheI NotI NruI NsiI
NspI NspV PacI PaeR7I PflFI
PinAI PleI PmaCI PmeI PmlI
PshAI Psp1406I PvuII RsrII SacI
SacII SapI SbfI ScaI SexAI
SfiI SfoI SfuI SgrAI SmaI
SmlI SnaBI SpeI SphI Sse8387I
StuI Tth111I XbaI XhoI XmaCI
XmaI XmnI
###################################
# ONE CUT ENZYMES ( 52 enzymes) #
###################################
AccI 66 GTCGAC GT^[AC][GT]AC
AcsI 892 GAATTC [GA]^AATT[CT]
AflIII 140 ACGTGT A^C[GA][CT]GT
AlwNI 1032 CAGGATCTG CAG[ACGT][ACGT][ACGT]^CTG
ApoI 892 GAATTC [GA]^AATT[CT]
AseI 462 ATTAAT AT^TAAT
AsnI 462 ATTAAT AT^TAAT
BanI 922 GGTGCC G^G[CT][GA]CC
BbsI 1310 GAAGAC GAAGAC(2/6)
BcgI 1014 CGAGACCATTGC (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
BclI 372 TGATCA T^GATCA
BfaI 898 CTAG C^TAG
BglII 1429 AGATCT A^GATCT
BmyI 963 GGGCAC G[AGT]GC[ACT]^C
BpuAI 1310 GAAGAC GAAGAC(2/6)
BsaI 1015 GAGACC GGTCTC(1/5)
BsgI 1403 GTGCAG GTGCAG(16/14)
Bsp1286I 963 GGGCAC G[AGT]GC[ACT]^C
Bsp1407I 210 TGTACA T^GTACA
BspHI 986 TCATGA T^CATGA
BsrBI 16 GAGCGG CCGCTC(-3/-3)
BsrGI 210 TGTACA T^GTACA
BstAPI 388 GCAAATGCTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
BstEII 715 GGTTACC G^GT[ACGT]ACC
BstPI 715 GGTTACC G^GT[ACGT]ACC
DraI 645 TTTAAA TTT^AAA
DraII 125 GGGACCC [GA]G^G[ACGT]CC[CT]
DraIII 1277 CACAAGGTG CAC[ACGT][ACGT][ACGT]^GTG
EcoO109I 125 GGGACCC [GA]G^G[ACGT]CC[CT]
EcoO65I 715 GGTTACC G^GT[ACGT]ACC
EcoRI 892 GAATTC G^AATTC
EcoRV 901 GATATC GAT^ATC
EcoT14I 951 CCTTGG C^C[AT][AT]GG
FauI 114 CCCGC CCCGC(4/6)
FbaI 372 TGATCA T^GATCA
HaeII 56 GGCGCT [GA]GCGC^[CT]
HincII 66 GTCGAC GT[CT]^[GA]AC
HindII 66 GTCGAC GT[CT]^[GA]AC
MaeI 898 CTAG C^TAG
MaeII 140 ACGT A^CGT
MslI 330 CATTACAATG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
PpuMI 125 GGGACCC [GA]G^G[AT]CC[CT]
PshBI 462 ATTAAT AT^TAAT
PstI 1038 CTGCAG CTGCA^G
PvuI 159 CGATCG CGAT^CG
RcaI 986 TCATGA T^CATGA
SalI 66 GTCGAC G^TCGAC
SspBI 210 TGTACA T^GTACA
StyI 951 CCTTGG C^C[AT][AT]GG
SwaI 644 ATTTAAAT ATTT^AAAT
Tsp45I 1273 GTGAC ^GT[GC]AC
XcmI 1094 CCAACCTCTGGCTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
###################################
# TWO CUT ENZYMES ( 17 enzymes) #
###################################
AlwI 90 GATCC GGATC(4/5)
1034 GGATC
BbvCI 1063 CCTCAGC CCTCAGC(-5/-2)
916 GCTGAGG
BcnI 76 CCGGG CC^[GC]GG
123 CCGGG
BsaJI 131 CCCAGG C^C[ACGT][ACGT]GG
951 CCTTGG
BsiEI 22 CGGTCG CG[GA][CT]^CG
159 CGATCG
BsmFI 125 GGGAC GGGAC(10/14)
1112 GGGAC
BsrI 364 ACTGG ACTGG(1/-1)
1358 CCAGT
HapII 76 CCGG C^CGG
123 CCGG
HinfI 759 GAATC G^A[ACGT]TC
1218 GAATC
HpaII 76 CCGG C^CGG
123 CCGG
MspI 76 CCGG C^CGG
123 CCGG
NciI 76 CCGGG CC^[GC]GG
123 CCGGG
PflMI 1019 CCATTGCGTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
1094 CCAACCTCTGG
SfcI 183 CTACAG C^T[GA][CT]AG
1038 CTGCAG
SspI 459 AATATT AAT^ATT
749 AATATT
TfiI 759 GAATC G^A[AT]TC
1218 GAATC
Van91I 1019 CCATTGCGTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
1094 CCAACCTCTGG
#########################################
# 3 OR MORE CUT ENZYMES ( 52 enzymes) #
#########################################
AccII AciI AfaI AluI AvaII
BbvI BsiYI BslI BsmAI BsrDI
BssKI BstF5I BstNI BstUI BstYI
Cac8I CfoI Cfr13I DdeI DpnII
EcoRII Fnu4HI FokI HaeIII HhaI
HinP1I HphI ItaI MaeIII MboI
MboII MflI MnlI MseI MvaI
MvnI MwoI NdeII NlaIII NlaIV
RsaI Sau3AI Sau96I ScrFI SfaNI
TaqI Tru9I TseI Tsp509I TspRI
TthHB8I XhoII