##################################
# NO CUT ENZYMES ( 131 enzymes) #
##################################
AatII Acc65I AccI AccIII AclI
AcsI AflII AflIII AgeI AhdI
Alw44I AocI Aor51HI ApaLI ApoI
AseI AsnI Asp718I AspEI AspI
AviII AvrII BalI BamHI BbrPI
BbvCI BciVI BfaI BfrI BglII
BlnI BsaAI BsaBI BsaI BseAI
BsgI BsiWI BsmAI BsmBI BsmFI
Bsp1407I BspDI BspEI BspHI BspLU11I
BspMI BsrDI BsrGI BssSI Bst1107I
BstAPI BstBI BstEII BstPI BstXI
BstYI BstZ17I Bsu36I ClaI CpoI
DraI DrdI Eam1105I Eco47III Eco81I
EcoO65I EcoRI EcoRV EcoT22I FseI
FspI HincII HindII HindIII HpaI
KpnI MaeI MamI MfeI MflI
MluI MluNI MroI MscI MunI
NdeI NheI NotI NruI NsiI
NspI NspV PacI PaeR7I PflFI
PinAI PmaCI PmeI PmlI PshAI
PshBI Psp1406I PvuI PvuII RcaI
RsrII SacI SalI SapI SbfI
ScaI SexAI SfaNI SfuI SgrAI
SmaI SnaBI SpeI SphI Sse8387I
SspBI SspI StuI SwaI Tsp509I
Tth111I XbaI XhoI XhoII XmaCI
XmaI
###################################
# ONE CUT ENZYMES ( 46 enzymes) #
###################################
AfaI 204 GTAC GT^AC
AlwI 3 GATCC GGATC(4/5)
AlwNI 449 CAGATACTG CAG[ACGT][ACGT][ACGT]^CTG
ApaI 956 GGGCCC GGGCC^C
AscI 328 GGCGCGCC GG^CGCGCC
Asp700I 916 GAATACCTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
AspHI 304 GAGCAC G[AT]GC[AT]^C
AvaII 906 GGACC G^G[AT]CC
BbsI 650 GTCTTC GAAGAC(2/6)
BcgI 80 CGAAGCGGGTGC (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
BclI 891 TGATCA T^GATCA
BlpI 243 GCTCAGC GC^T[ACGT]AGC
BpmI 544 CTCCAG CTGGAG(16/14)
Bpu1102I 243 GCTCAGC GC^T[ACGT]AGC
BpuAI 650 GTCTTC GAAGAC(2/6)
BsaWI 658 ACCGGA [AT]^CCGG[AT]
BsiHKAI 304 GAGCAC G[AT]GC[AT]^C
BsmI 483 GCATTC GAATGC(1/-1)
BsrBI 163 CCGCTC CCGCTC(-3/-3)
BsrFI 965 GCCGGC [GA]^CCGG[CT]
BsrI 1030 ACTGG ACTGG(1/-1)
BssHII 329 GCGCGC G^CGCGC
CelII 243 GCTCAGC GC^T[ACGT]AGC
Cfr10I 965 GCCGGC [GA]^CCGG[CT]
DraIII 232 CACAACGTG CAC[ACGT][ACGT][ACGT]^GTG
EcoNI 740 CCTACGCCAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
EcoT14I 23 CCATGG C^C[AT][AT]GG
FbaI 891 TGATCA T^GATCA
MseI 466 TTAA T^TAA
MslI 565 CATACAGGTG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
NaeI 965 GCCGGC GCC^GGC
NcoI 23 CCATGG C^CATGG
NgoMIV 965 GCCGGC G^CCGGC
PflMI 598 CCAGACTGTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
PpuMI 905 AGGACCC [GA]G^G[AT]CC[CT]
RsaI 204 GTAC GT^AC
SfiI 255 GGCCGCGGGGGCC GGCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGCC
SmlI 415 CTCAAG C^T[CT][GA]AG
StyI 23 CCATGG C^C[AT][AT]GG
TaqI 14 TCGA T^CGA
Tru9I 466 TTAA T^TAA
TspRI 724 CACTG CA[GC]TG[ACGT][ACGT]^
TthHB8I 14 TCGA T^CGA
Van91I 598 CCAGACTGTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
XcmI 411 CCACCTCAAGGATGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
XmnI 916 GAATACCTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
###################################
# TWO CUT ENZYMES ( 22 enzymes) #
###################################
AcyI 18 GGCGCC G[GA]^CG[CT]C
1035 GGCGCC
AvaI 309 CTCGGG C^[CT]CG[GA]G
366 CTCGGG
BanI 18 GGCGCC G^G[CT][GA]CC
1035 GGCGCC
BbeI 18 GGCGCC GGCGC^C
1035 GGCGCC
BsaHI 18 GGCGCC G[GA]^CG[CT]C
1035 GGCGCC
BseRI 382 GAGGAG GAGGAG(10/8)
760 GAGGAG
BsiEI 275 CGGCCG CG[GA][CT]^CG
812 CGGCCG
BsoBI 309 CTCGGG C^[CT]CG[GA]G
366 CTCGGG
EagI 275 CGGCCG C^GGCCG
812 CGGCCG
EclXI 275 CGGCCG C^GGCCG
812 CGGCCG
Eco52I 275 CGGCCG C^GGCCG
812 CGGCCG
HgaI 240 GACGC GACGC(5/10)
648 GCGTC
Hin1I 18 GGCGCC G[GA]^CG[CT]C
1035 GGCGCC
KasI 18 GGCGCC G^GCGCC
1035 GGCGCC
KspI 257 CCGCGG CCGC^GG
809 CCGCGG
NarI 18 GGCGCC GG^CGCC
1035 GGCGCC
PleI 73 GACTC GAGTC(4/5)
561 GACTC
PstI 287 CTGCAG CTGCA^G
970 CTGCAG
SacII 257 CCGCGG CCGC^GG
809 CCGCGG
SfcI 287 CTGCAG C^T[GA][CT]AG
970 CTGCAG
SfoI 18 GGCGCC GGC^GCC
1035 GGCGCC
Tsp45I 238 GTGAC ^GT[GC]AC
590 GTGAC
#########################################
# 3 OR MORE CUT ENZYMES ( 59 enzymes) #
#########################################
AccII AciI AluI BanII BbvI
BcnI BglI BmyI BsaJI BsiYI
BslI Bsp1286I BssKI BstF5I BstNI
BstUI Cac8I CfoI Cfr13I DdeI
DpnII DraII DsaI EaeI EarI
EcoO109I EcoRII FauI Fnu4HI FokI
HaeII HaeIII HapII HhaI HinP1I
HinfI HpaII HphI ItaI Ksp632I
MaeII MaeIII MboI MboII MnlI
MspA1I MspI MvaI MvnI MwoI
NciI NdeII NlaIII NlaIV Sau3AI
Sau96I ScrFI TfiI TseI