Restriction map for
pFN21AE6373
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# NO CUT ENZYMES ( 149 enzymes) #
##################################
AatII Acc65I AccI AccIII AclI
AcyI AflII AflIII AgeI AhdI
Alw44I AlwI AlwNI AocI Aor51HI
ApaI ApaLI AscI Asp700I Asp718I
AspEI AspI AviII AvrII BalI
BamHI BanI BbeI BbrPI BbsI
BbvCI BcgI BclI BfrI BglI
BglII BlnI BlpI Bpu1102I BpuAI
BsaAI BsaHI BsaWI BseAI BsiWI
BsmBI BsmI Bsp1407I BspDI BspEI
BspLU11I BsrBI BsrDI BsrGI BssHII
BssSI Bst1107I BstAPI BstBI BstEII
BstPI BstYI BstZ17I Bsu36I CelII
ClaI CpoI DpnII DraI DraIII
DrdI Eam1105I Eco47III Eco81I EcoO65I
EcoT22I FbaI FseI FspI HgaI
Hin1I HindIII HinfI HpaI KasI
KpnI KspI MboI MfeI MflI
MluI MluNI MroI MscI MunI
NarI NcoI NdeI NdeII NheI
NotI NruI NsiI NspI NspV
PacI PaeR7I PflFI PflMI PinAI
PleI PmaCI PmeI PmlI PshAI
Psp1406I PstI PvuI RsrII SacI
SacII SalI Sau3AI SbfI SexAI
SfaNI SfiI SfoI SfuI SgrAI
SmaI SnaBI SpeI SphI Sse8387I
SspBI StuI SwaI TaqI TfiI
Tth111I TthHB8I Van91I XbaI XhoI
XhoII XmaCI XmaI XmnI
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# ONE CUT ENZYMES ( 55 enzymes) #
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AseI 858 ATTAAT AT^TAAT
AsnI 858 ATTAAT AT^TAAT
AspHI 364 GTGCTC G[AT]GC[AT]^C
AvaI 466 CTCGGG C^[CT]CG[GA]G
BanII 43 GAGCCC G[GA]GC[CT]^C
BciVI 835 GTATCC GTATCC(6/5)
BfaI 595 CTAG C^TAG
BpmI 668 CTGGAG CTGGAG(16/14)
BsaBI 855 GATATTAATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
BsaI 671 GAGACC GGTCTC(1/5)
BseRI 295 CTCCTC GAGGAG(10/8)
BsgI 780 GTGCAG GTGCAG(16/14)
BsiEI 5 CGGCCG CG[GA][CT]^CG
BsiHKAI 364 GTGCTC G[AT]GC[AT]^C
BsmFI 103 GGGAC GGGAC(10/14)
BsoBI 466 CTCGGG C^[CT]CG[GA]G
BspHI 632 TCATGA T^CATGA
BsrFI 7 GCCGGC [GA]^CCGG[CT]
BstF5I 441 CATCC GGATG(2/0)
BstNI 15 CCAGG CC^[AT]GG
BstXI 312 CCACATAAGTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
Cfr10I 7 GCCGGC [GA]^CCGG[CT]
DraII 103 GGGACCT [GA]G^G[ACGT]CC[CT]
DsaI 770 CCACGG C^C[GA][CT]GG
EagI 5 CGGCCG C^GGCCG
EclXI 5 CGGCCG C^GGCCG
Eco52I 5 CGGCCG C^GGCCG
EcoNI 675 CCTGCAACAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
EcoO109I 103 GGGACCT [GA]G^G[ACGT]CC[CT]
EcoRI 691 GAATTC G^AATTC
EcoRII 15 CCAGG ^CC[AT]GG
EcoRV 538 GATATC GAT^ATC
EcoT14I 177 CCTTGG C^C[AT][AT]GG
FokI 441 CATCC GGATG(9/13)
HincII 130 GTCAAC GT[CT]^[GA]AC
HindII 130 GTCAAC GT[CT]^[GA]AC
MaeI 595 CTAG C^TAG
MaeII 302 ACGT A^CGT
MaeIII 165 GTCAC ^GT[ACGT]AC
MamI 855 GATATTAATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
MspA1I 482 CAGCTG C[AC]G^C[GT]G
MvaI 15 CCAGG CC^[AT]GG
NaeI 7 GCCGGC GCC^GGC
NgoMIV 7 GCCGGC G^CCGGC
PpuMI 103 GGGACCT [GA]G^G[AT]CC[CT]
PshBI 858 ATTAAT AT^TAAT
PvuII 482 CAGCTG CAG^CTG
RcaI 632 TCATGA T^CATGA
ScaI 609 AGTACT AGT^ACT
SfcI 724 CTACAG C^T[GA][CT]AG
SmlI 927 CTTGAG C^T[CT][GA]AG
SspI 907 AATATT AAT^ATT
StyI 177 CCTTGG C^C[AT][AT]GG
Tsp45I 165 GTCAC ^GT[GC]AC
XcmI 569 CCAAACAGAAAATGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
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# TWO CUT ENZYMES ( 16 enzymes) #
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AfaI 610 GTAC GT^AC
765 GTAC
AvaII 104 GGACC G^G[AT]CC
582 GGACC
BcnI 46 CCCGG CC^[GC]GG
77 CCCGG
BmyI 43 GAGCCC G[AGT]GC[ACT]^C
364 GTGCTC
Bsp1286I 43 GAGCCC G[AGT]GC[ACT]^C
364 GTGCTC
BspMI 106 ACCTGC ACCTGC(4/8)
674 ACCTGC
BsrI 667 ACTGG ACTGG(1/-1)
957 CCAGT
EaeI 5 CGGCCG [CT]^GGCC[GA]
12 CGGCCA
EarI 94 CTCTTC CTCTTC(1/4)
1005 GAAGAG
FauI 38 GCGGG CCCGC(4/6)
69 GCGGG
Ksp632I 94 CTCTTC CTCTTC(1/4)
1005 GAAGAG
MslI 201 CACCATAATG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
313 CACATAAGTG
NciI 46 CCCGG CC^[GC]GG
77 CCCGG
NlaIII 633 CATG CATG^
915 CATG
RsaI 610 GTAC GT^AC
765 GTAC
SapI 1005 GAAGAGC GCTCTTC(1/4)
93 GCTCTTC
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# 3 OR MORE CUT ENZYMES ( 38 enzymes) #
#########################################
AccII AciI AcsI AluI ApoI
BbvI BsaJI BsiYI BslI BsmAI
BssKI BstUI Cac8I CfoI Cfr13I
DdeI Fnu4HI HaeII HaeIII HapII
HhaI HinP1I HpaII HphI ItaI
MboII MnlI MseI MspI MvnI
MwoI NlaIV Sau96I ScrFI Tru9I
TseI Tsp509I TspRI