Restriction map for
pFN21AE3213
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# NO CUT ENZYMES ( 113 enzymes) #
##################################
AatII AccIII AclI AcsI AcyI
AflII AflIII AgeI AhdI AlwI
ApoI AscI AseI AsnI AspEI
AspI AviII AvrII BamHI BbeI
BbvCI BcgI BfaI BfrI BglII
BlnI BlpI Bpu1102I BsaBI BsaHI
BsaI BsaWI BseAI BsiWI BsmBI
BspDI BspEI BspLU11I BsrBI BsrDI
BssHII BssSI Bst1107I BstAPI BstBI
BstYI BstZ17I CelII ClaI CpoI
DraIII DrdI Eam1105I EcoNI EcoRI
EcoRV EcoT22I FseI FspI Hin1I
HindIII HpaI KasI KspI MaeI
MamI MfeI MflI MluI MroI
MspA1I MunI NaeI NarI NcoI
NdeI NgoMIV NheI NotI NruI
NsiI NspV PacI PflFI PinAI
PmeI PshBI Psp1406I PvuI PvuII
RsrII SacI SacII SapI SbfI
ScaI SexAI SfiI SfoI SfuI
SgrAI SmaI SnaBI SpeI Sse8387I
SspI SwaI Tth111I XbaI XcmI
XhoII XmaCI XmaI
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# ONE CUT ENZYMES ( 44 enzymes) #
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Acc65I 35 GGTACC G^GTACC
AccI 1422 GTCGAC GT^[AC][GT]AC
Alw44I 784 GTGCAC G^TGCAC
AlwNI 1111 CAGAAGCTG CAG[ACGT][ACGT][ACGT]^CTG
AocI 859 CCTCAGG CC^T[ACGT]AGG
ApaLI 784 GTGCAC G^TGCAC
Asp718I 35 GGTACC G^GTACC
BalI 740 TGGCCA TGG^CCA
BbrPI 787 CACGTG CAC^GTG
BciVI 315 GGATAC GTATCC(6/5)
BglI 827 GCCGGGCAGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
BseRI 1060 GAGGAG GAGGAG(10/8)
BsiEI 388 CGGCCG CG[GA][CT]^CG
BsrFI 386 ACCGGC [GA]^CCGG[CT]
BstEII 941 GGTTACC G^GT[ACGT]ACC
BstPI 941 GGTTACC G^GT[ACGT]ACC
BstXI 731 CCAACGGCCTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
Bsu36I 859 CCTCAGG CC^T[ACGT]AGG
Cfr10I 386 ACCGGC [GA]^CCGG[CT]
DraI 267 TTTAAA TTT^AAA
EagI 388 CGGCCG C^GGCCG
EclXI 388 CGGCCG C^GGCCG
Eco52I 388 CGGCCG C^GGCCG
Eco81I 859 CCTCAGG CC^T[ACGT]AGG
EcoO65I 941 GGTTACC G^GT[ACGT]ACC
FauI 867 CCCGC CCCGC(4/6)
HphI 640 GGTGA GGTGA(8/7)
KpnI 35 GGTACC GGTAC^C
MluNI 740 TGGCCA TGG^CCA
MscI 740 TGGCCA TGG^CCA
MslI 795 CACACGGGTG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
NspI 485 GCATGC [GA]CATG^[CT]
PaeR7I 438 CTCGAG C^TCGAG
PflMI 415 CCAAAATGTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
PmaCI 787 CACGTG CAC^GTG
PmlI 787 CACGTG CAC^GTG
PpuMI 308 AGGACCT [GA]G^G[AT]CC[CT]
PshAI 287 GACCAGAGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC
SalI 1422 GTCGAC G^TCGAC
SphI 485 GCATGC GCATG^C
TfiI 393 GATTC G^A[AT]TC
Tsp509I 349 AATT ^AATT
Van91I 415 CCAAAATGTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
XhoI 438 CTCGAG C^TCGAG
###################################
# TWO CUT ENZYMES ( 34 enzymes) #
###################################
Aor51HI 80 AGCGCT AGC^GCT
932 AGCGCT
ApaI 748 GGGCCC GGGCC^C
864 GGGCCC
Asp700I 711 GAAGCCGTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
1048 GAAGAGTTTC
AspHI 382 GAGCAC G[AT]GC[AT]^C
784 GTGCAC
BanI 35 GGTACC G^G[CT][GA]CC
835 GGCACC
BbsI 126 GAAGAC GAAGAC(2/6)
956 GTCTTC
BpmI 973 CTCCAG CTGGAG(16/14)
687 CTGGAG
BpuAI 126 GAAGAC GAAGAC(2/6)
956 GTCTTC
BsaAI 787 CACGTG [CT]AC^GT[GA]
1397 TACGTG
BsiHKAI 382 GAGCAC G[AT]GC[AT]^C
784 GTGCAC
BsmAI 294 GTCTC GTCTC(1/5)
1227 GTCTC
BsmI 575 GAATGC GAATGC(1/-1)
1340 GCATTC
Bsp1407I 962 TGTACA T^GTACA
1022 TGTACA
BspHI 185 TCATGA T^CATGA
1214 TCATGA
BspMI 152 ACCTGC ACCTGC(4/8)
992 ACCTGC
BsrGI 962 TGTACA T^GTACA
1022 TGTACA
BstF5I 1137 GGATG GGATG(2/0)
1242 GGATG
DsaI 39 CCACGG C^C[GA][CT]GG
677 CCGTGG
EaeI 388 CGGCCG [CT]^GGCC[GA]
740 TGGCCA
Eco47III 80 AGCGCT AGC^GCT
932 AGCGCT
EcoT14I 912 CCAAGG C^C[AT][AT]GG
1199 CCAAGG
FokI 1137 GGATG GGATG(9/13)
1242 GGATG
HaeII 80 AGCGCT [GA]GCGC^[CT]
932 AGCGCT
HgaI 86 GCGTC GACGC(5/10)
1184 GCGTC
HincII 1375 GTCAAC GT[CT]^[GA]AC
1422 GTCGAC
HindII 1375 GTCAAC GT[CT]^[GA]AC
1422 GTCGAC
MaeII 788 ACGT A^CGT
1398 ACGT
RcaI 185 TCATGA T^CATGA
1214 TCATGA
SfaNI 664 GATGC GCATC(5/9)
1243 GATGC
SspBI 962 TGTACA T^GTACA
1022 TGTACA
StuI 1008 AGGCCT AGG^CCT
1261 AGGCCT
StyI 912 CCAAGG C^C[AT][AT]GG
1199 CCAAGG
Tsp45I 302 GTGAC ^GT[GC]AC
448 GTCAC
XmnI 711 GAAGCCGTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
1048 GAAGAGTTTC
#########################################
# 3 OR MORE CUT ENZYMES ( 67 enzymes) #
#########################################
AccII AciI AfaI AluI AvaI
AvaII BanII BbvI BclI BcnI
BmyI BsaJI BsgI BsiYI BslI
BsmFI BsoBI Bsp1286I BsrI BssKI
BstNI BstUI Cac8I CfoI Cfr13I
DdeI DpnII DraII EarI EcoO109I
EcoRII FbaI Fnu4HI HaeIII HapII
HhaI HinP1I HinfI HpaII ItaI
Ksp632I MaeIII MboI MboII MnlI
MseI MspI MvaI MvnI MwoI
NciI NdeII NlaIII NlaIV PleI
PstI RsaI Sau3AI Sau96I ScrFI
SfcI SmlI TaqI Tru9I TseI
TspRI TthHB8I