Restriction map for
pFN21AE3288
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# NO CUT ENZYMES ( 93 enzymes) #
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Acc65I AccIII AclI AflII AgeI
AhdI AocI Aor51HI ApaI AscI
Asp700I Asp718I AspEI AspI AviII
AvrII BbrPI BfrI BglI BlnI
BlpI Bpu1102I BsaAI BsaBI BsaI
BseAI BsiWI BsmBI BsmI Bsp1407I
BspDI BspEI BsrGI BssHII Bst1107I
BstAPI BstBI BstZ17I Bsu36I CelII
ClaI CpoI DraI DrdI Eam1105I
Eco47III Eco81I EcoNI EcoRI EcoRV
EcoT22I FspI HpaI KpnI MamI
MfeI MroI MunI NcoI NheI
NotI NruI NsiI NspV PacI
PflFI PinAI PmaCI PmeI PmlI
Psp1406I PvuI RsrII SacI SalI
SbfI ScaI SfiI SfuI SgrAI
SmaI SnaBI SpeI SphI Sse8387I
SspBI SspI StuI SwaI Tth111I
XmaCI XmaI XmnI
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# ONE CUT ENZYMES ( 52 enzymes) #
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AatII 707 GACGTC GACGT^C
AccI 1320 GTCTAC GT^[AC][GT]AC
Alw44I 108 GTGCAC G^TGCAC
ApaLI 108 GTGCAC G^TGCAC
AseI 483 ATTAAT AT^TAAT
AsnI 483 ATTAAT AT^TAAT
AspHI 108 GTGCAC G[AT]GC[AT]^C
BamHI 258 GGATCC G^GATCC
BanII 578 GAGCCC G[GA]GC[CT]^C
BbsI 46 GTCTTC GAAGAC(2/6)
BbvCI 682 GCTGAGG CCTCAGC(-5/-2)
BcgI 2106 GCACCTTGCTCG (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
BciVI 1785 GGATAC GTATCC(6/5)
BclI 936 TGATCA T^GATCA
BpmI 82 CTGGAG CTGGAG(16/14)
BpuAI 46 GTCTTC GAAGAC(2/6)
BsaWI 1037 ACCGGA [AT]^CCGG[AT]
BsiHKAI 108 GTGCAC G[AT]GC[AT]^C
BspHI 1673 TCATGA T^CATGA
BspMI 738 GCAGGT ACCTGC(4/8)
BsrFI 129 GCCGGC [GA]^CCGG[CT]
BssSI 351 CTCGTG CACGAG(-5/-1)
BstEII 741 GGTGACC G^GT[ACGT]ACC
BstPI 741 GGTGACC G^GT[ACGT]ACC
BstXI 1943 CCATTATGCTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
Cfr10I 129 GCCGGC [GA]^CCGG[CT]
DsaI 225 CCGCGG C^C[GA][CT]GG
EcoO65I 741 GGTGACC G^GT[ACGT]ACC
EcoT14I 1506 CCAAGG C^C[AT][AT]GG
FbaI 936 TGATCA T^GATCA
FseI 128 GGCCGGCC GGCCGG^CC
HincII 1282 GTTGAC GT[CT]^[GA]AC
HindII 1282 GTTGAC GT[CT]^[GA]AC
HindIII 1881 AAGCTT A^AGCTT
KspI 225 CCGCGG CCGC^GG
MluI 783 ACGCGT A^CGCGT
NaeI 129 GCCGGC GCC^GGC
NdeI 494 CATATG CA^TATG
NgoMIV 129 GCCGGC G^CCGGC
PaeR7I 2114 CTCGAG C^TCGAG
PflMI 153 CCAGCGGCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
PshAI 244 GACTGGGGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC
PshBI 483 ATTAAT AT^TAAT
PvuII 1790 CAGCTG CAG^CTG
RcaI 1673 TCATGA T^CATGA
SacII 225 CCGCGG CCGC^GG
SexAI 1580 ACCTGGT A^CC[AT]GGT
StyI 1506 CCAAGG C^C[AT][AT]GG
Van91I 153 CCAGCGGCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
XbaI 1147 TCTAGA T^CTAGA
XcmI 393 CCAGGCGCGACTTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
XhoI 2114 CTCGAG C^TCGAG
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# TWO CUT ENZYMES ( 15 enzymes) #
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AlwNI 154 CAGCGGCTG CAG[ACGT][ACGT][ACGT]^CTG
940 CAGAGTCTG
BglII 732 AGATCT A^GATCT
1105 AGATCT
BsgI 2104 CTGCAC GTGCAG(16/14)
1399 GTGCAG
BspLU11I 501 ACATGT A^CATGT
1870 ACATGT
BsrBI 69 CCGCTC CCGCTC(-3/-3)
1209 CCGCTC
BsrDI 564 CATTGC GCAATG(2/0)
2213 GCAATG
DraII 217 GGGTCCC [GA]G^G[ACGT]CC[CT]
249 GGGTCCT
DraIII 348 CACCTCGTG CAC[ACGT][ACGT][ACGT]^GTG
1393 CACGGGGTG
EcoO109I 217 GGGTCCC [GA]G^G[ACGT]CC[CT]
249 GGGTCCT
MaeII 708 ACGT A^CGT
2041 ACGT
MslI 543 CACAGAAATG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
564 CATTGCGGTG
NspI 501 ACATGT [GA]CATG^[CT]
1870 ACATGT
PpuMI 217 GGGTCCC [GA]G^G[AT]CC[CT]
249 GGGTCCT
SapI 433 GAAGAGC GCTCTTC(1/4)
1211 GCTCTTC
TfiI 636 GATTC G^A[AT]TC
1805 GATTC
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# 3 OR MORE CUT ENZYMES ( 98 enzymes) #
#########################################
AccII AciI AcsI AcyI AfaI
AflIII AluI AlwI ApoI AvaI
AvaII BalI BanI BbeI BbvI
BcnI BfaI BmyI BsaHI BsaJI
BseRI BsiEI BsiYI BslI BsmAI
BsmFI BsoBI Bsp1286I BsrI BssKI
BstF5I BstNI BstUI BstYI Cac8I
CfoI Cfr13I DdeI DpnII EaeI
EagI EarI EclXI Eco52I EcoRII
FauI Fnu4HI FokI HaeII HaeIII
HapII HgaI HhaI Hin1I HinP1I
HinfI HpaII HphI ItaI KasI
Ksp632I MaeI MaeIII MboI MboII
MflI MluNI MnlI MscI MseI
MspA1I MspI MvaI MvnI MwoI
NarI NciI NdeII NlaIII NlaIV
PleI PstI RsaI Sau3AI Sau96I
ScrFI SfaNI SfcI SfoI SmlI
TaqI Tru9I TseI Tsp45I Tsp509I
TspRI TthHB8I XhoII