Restriction map for
pFN21AE3291
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# NO CUT ENZYMES ( 123 enzymes) #
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Acc65I AccII AclI AflII AgeI
AhdI Alw44I Aor51HI ApaI ApaLI
AscI Asp700I Asp718I AspEI AspHI
AspI AviII AvrII BamHI BanI
BanII BbeI BbrPI BbsI BbvCI
BciVI BfrI BglI BglII BlnI
BlpI BmyI BpmI Bpu1102I BpuAI
BsaAI BsaI BseRI BsiEI BsiHKAI
BsiWI BsmBI Bsp1286I Bsp1407I BspLU11I
BsrBI BsrFI BsrGI BssHII BssSI
BstBI BstEII BstPI BstUI BstXI
CelII Cfr10I CpoI DraI DrdI
EagI Eam1105I EclXI Eco47III Eco52I
EcoO65I EcoRI EcoT22I FseI FspI
HaeII HindIII HpaI KasI KpnI
KspI MluI MvnI NaeI NarI
NdeI NgoMIV NheI NotI NruI
NsiI NspV PacI PaeR7I PflFI
PflMI PinAI PmaCI PmeI PmlI
Psp1406I PvuI RsrII SacI SacII
SalI SapI SbfI ScaI SexAI
SfiI SfoI SfuI SgrAI SmaI
SnaBI SpeI SphI Sse8387I SspBI
SwaI Tth111I Van91I XbaI XhoI
XmaCI XmaI XmnI
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# ONE CUT ENZYMES ( 45 enzymes) #
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AatII 2134 GACGTC GACGT^C
AccIII 2095 TCCGGA T^CCGGA
AcyI 2134 GACGTC G[GA]^CG[CT]C
AocI 1590 CCTTAGG CC^T[ACGT]AGG
AseI 1429 ATTAAT AT^TAAT
AsnI 1429 ATTAAT AT^TAAT
AvaI 1532 CTCGGG C^[CT]CG[GA]G
BcgI 1721 GCACAGGAATCG (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
BclI 1037 TGATCA T^GATCA
BcnI 1095 CCGGG CC^[GC]GG
BsaBI 468 GATGAACATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
BsaHI 2134 GACGTC G[GA]^CG[CT]C
BsaWI 2095 TCCGGA [AT]^CCGG[AT]
BseAI 2095 TCCGGA T^CCGGA
BsoBI 1532 CTCGGG C^[CT]CG[GA]G
BspDI 1222 ATCGAT AT^CGAT
BspEI 2095 TCCGGA T^CCGGA
BspMI 1913 ACCTGC ACCTGC(4/8)
BsrDI 1994 GCAATG GCAATG(2/0)
Bst1107I 1392 GTATAC GTA^TAC
BstAPI 1125 GCAAGCACTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
BstYI 2252 GGATCT [GA]^GATC[CT]
BstZ17I 1392 GTATAC GTA^TAC
Bsu36I 1590 CCTTAGG CC^T[ACGT]AGG
CfoI 1963 GCGC GCG^C
ClaI 1222 ATCGAT AT^CGAT
DraIII 751 CACCCTGTG CAC[ACGT][ACGT][ACGT]^GTG
Eco81I 1590 CCTTAGG CC^T[ACGT]AGG
FbaI 1037 TGATCA T^GATCA
HgaI 856 GACGC GACGC(5/10)
HhaI 1963 GCGC GCG^C
Hin1I 2134 GACGTC G[GA]^CG[CT]C
HinP1I 1963 GCGC G^CGC
HincII 2197 GTTGAC GT[CT]^[GA]AC
HindII 2197 GTTGAC GT[CT]^[GA]AC
MamI 468 GATGAACATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
MfeI 314 CAATTG C^AATTG
MflI 2252 GGATCT [GA]^GATC[CT]
MroI 2095 TCCGGA T^CCGGA
MunI 314 CAATTG C^AATTG
NciI 1095 CCGGG CC^[GC]GG
PshAI 831 GACCCTAGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC
PshBI 1429 ATTAAT AT^TAAT
SmlI 1283 CTCAAG C^T[CT][GA]AG
XhoII 2252 GGATCT [GA]^GATC[CT]
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# TWO CUT ENZYMES ( 23 enzymes) #
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AccI 1392 GTATAC GT^[AC][GT]AC
1633 GTAGAC
AfaI 226 GTAC GT^AC
1155 GTAC
AflIII 933 ACACGT A^C[GA][CT]GT
1051 ACACGT
BsmAI 425 GAGAC GTCTC(1/5)
1809 GTCTC
BsmI 774 GCATTC GAATGC(1/-1)
1551 GCATTC
BspHI 48 TCATGA T^CATGA
410 TCATGA
EarI 1064 GAAGAG CTCTTC(1/4)
1420 GAAGAG
EcoNI 1531 CCTCGGGAAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
1877 CCTTGCAGAGG
EcoRV 406 GATATC GAT^ATC
597 GATATC
HapII 1095 CCGG C^CGG
2096 CCGG
HpaII 1095 CCGG C^CGG
2096 CCGG
Ksp632I 1064 GAAGAG CTCTTC(1/4)
1420 GAAGAG
MspA1I 996 CAGCTG C[AC]G^C[GT]G
2024 CAGCTG
MspI 1095 CCGG C^CGG
2096 CCGG
NcoI 341 CCATGG C^CATGG
1842 CCATGG
NspI 534 ACATGC [GA]CATG^[CT]
698 GCATGT
PvuII 996 CAGCTG CAG^CTG
2024 CAGCTG
RcaI 48 TCATGA T^CATGA
410 TCATGA
RsaI 226 GTAC GT^AC
1155 GTAC
SspI 850 AATATT AAT^ATT
1119 AATATT
StuI 357 AGGCCT AGG^CCT
2150 AGGCCT
Tsp45I 757 GTGAC ^GT[GC]AC
2111 GTCAC
XcmI 539 CCATAACAAAACTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
2222 CCAGCATGGTTCTGG
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# 3 OR MORE CUT ENZYMES ( 67 enzymes) #
#########################################
AciI AcsI AluI AlwI AlwNI
ApoI AvaII BalI BbvI BfaI
BsaJI BsgI BsiYI BslI BsmFI
BsrI BssKI BstF5I BstNI Cac8I
Cfr13I DdeI DpnII DraII DsaI
EaeI EcoO109I EcoRII EcoT14I FauI
Fnu4HI FokI HaeIII HinfI HphI
ItaI MaeI MaeII MaeIII MboI
MboII MluNI MnlI MscI MseI
MslI MvaI MwoI NdeII NlaIII
NlaIV PleI PpuMI PstI Sau3AI
Sau96I ScrFI SfaNI SfcI StyI
TaqI TfiI Tru9I TseI Tsp509I
TspRI TthHB8I