Restriction map for
pFN21AE5430
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# NO CUT ENZYMES ( 153 enzymes) #
##################################
AatII Acc65I AccI AccII AccIII
AclI AcsI AcyI AflII AflIII
AgeI AhdI Alw44I Aor51HI ApaI
ApaLI ApoI AscI AseI AsnI
Asp700I Asp718I AspEI AvaI AviII
BanI BanII BbeI BbrPI BcgI
BciVI BfrI BglI BglII BlpI
BpmI Bpu1102I BsaAI BsaBI BsaHI
BsaWI BseAI BsiEI BsiWI BsmBI
BsmI BsoBI Bsp1407I BspDI BspEI
BspLU11I BsrBI BsrFI BsrGI BssHII
Bst1107I BstAPI BstBI BstUI BstXI
BstZ17I CelII CfoI Cfr10I ClaI
CpoI DraI DraIII DrdI EagI
Eam1105I EarI EclXI Eco47III Eco52I
EcoNI EcoRI EcoRV EcoT22I FseI
FspI HaeII HgaI HhaI Hin1I
HinP1I HincII HindII HindIII HpaI
KasI KpnI Ksp632I KspI MaeII
MamI MfeI MluI MroI MseI
MunI MvnI NaeI NarI NdeI
NgoMIV NheI NotI NruI NsiI
NspV PacI PaeR7I PinAI PmaCI
PmeI PmlI PshAI PshBI Psp1406I
PstI PvuI RsrII SacI SacII
SalI SapI SbfI ScaI SexAI
SfiI SfoI SfuI SgrAI SmaI
SmlI SnaBI SpeI SphI Sse8387I
SspBI SspI StuI SwaI TaqI
Tru9I TthHB8I XbaI XcmI XhoI
XmaCI XmaI XmnI
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# ONE CUT ENZYMES ( 35 enzymes) #
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AciI 162 CCGC CCGC(-3/-1)
AlwNI 441 CAGCTCCTG CAG[ACGT][ACGT][ACGT]^CTG
AspHI 88 GTGCTC G[AT]GC[AT]^C
AspI 514 GACAATGTC GAC[ACGT]^[ACGT][ACGT]GTC
BalI 374 TGGCCA TGG^CCA
BamHI 683 GGATCC G^GATCC
BcnI 630 CCCGG CC^[GC]GG
BmyI 88 GTGCTC G[AGT]GC[ACT]^C
BsiHKAI 88 GTGCTC G[AT]GC[AT]^C
Bsp1286I 88 GTGCTC G[AGT]GC[ACT]^C
BspHI 407 TCATGA T^CATGA
BspMI 721 ACCTGC ACCTGC(4/8)
BsrDI 125 GCAATG GCAATG(2/0)
BsrI 240 ACTGG ACTGG(1/-1)
BssSI 109 CTCGTG CACGAG(-5/-1)
EaeI 374 TGGCCA [CT]^GGCC[GA]
FauI 161 CCCGC CCCGC(4/6)
HapII 631 CCGG C^CGG
HpaII 631 CCGG C^CGG
MluNI 374 TGGCCA TGG^CCA
MscI 374 TGGCCA TGG^CCA
MspI 631 CCGG C^CGG
MwoI 90 GCTCATCCTGC GC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GC
NciI 630 CCCGG CC^[GC]GG
NspI 425 ACATGC [GA]CATG^[CT]
PflFI 514 GACAATGTC GAC[ACGT]^[ACGT][ACGT]GTC
PflMI 440 CCAGCTCCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
RcaI 407 TCATGA T^CATGA
SfaNI 870 GATGC GCATC(5/9)
SfcI 888 CTATAG C^T[GA][CT]AG
TfiI 543 GATTC G^A[AT]TC
Tsp509I 492 AATT ^AATT
TspRI 239 CACTG CA[GC]TG[ACGT][ACGT]^
Tth111I 514 GACAATGTC GAC[ACGT]^[ACGT][ACGT]GTC
Van91I 440 CCAGCTCCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
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# TWO CUT ENZYMES ( 22 enzymes) #
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AvrII 189 CCTAGG C^CTAGG
621 CCTAGG
BbvCI 689 CCTCAGC CCTCAGC(-5/-2)
694 GCTGAGG
BclI 404 TGATCA T^GATCA
590 TGATCA
BfaI 190 CTAG C^TAG
622 CTAG
BlnI 189 CCTAGG C^CTAGG
621 CCTAGG
BsaI 16 GAGACC GGTCTC(1/5)
936 GAGACC
BseRI 228 CTCCTC GAGGAG(10/8)
924 GAGGAG
BsgI 166 CTGCAC GTGCAG(16/14)
298 GTGCAG
BstEII 144 GGTGACC G^GT[ACGT]ACC
861 GGTGACC
BstPI 144 GGTGACC G^GT[ACGT]ACC
861 GGTGACC
BstYI 683 GGATCC [GA]^GATC[CT]
822 GGATCT
DraII 131 GGGTCCT [GA]G^G[ACGT]CC[CT]
760 GGGACCT
EcoO109I 131 GGGTCCT [GA]G^G[ACGT]CC[CT]
760 GGGACCT
EcoO65I 144 GGTGACC G^GT[ACGT]ACC
861 GGTGACC
FbaI 404 TGATCA T^GATCA
590 TGATCA
HaeIII 375 GGCC GG^CC
564 GGCC
MaeI 190 CTAG C^TAG
622 CTAG
MflI 683 GGATCC [GA]^GATC[CT]
822 GGATCT
MspA1I 496 CAGCTG C[AC]G^C[GT]G
692 CAGCTG
PpuMI 131 GGGTCCT [GA]G^G[AT]CC[CT]
760 GGGACCT
PvuII 496 CAGCTG CAG^CTG
692 CAGCTG
XhoII 683 GGATCC [GA]^GATC[CT]
822 GGATCT
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# 3 OR MORE CUT ENZYMES ( 48 enzymes) #
#########################################
AfaI AluI AlwI AocI AvaII
BbsI BbvI BpuAI BsaJI BsiYI
BslI BsmAI BsmFI BssKI BstF5I
BstNI Bsu36I Cac8I Cfr13I DdeI
DpnII DsaI Eco81I EcoRII EcoT14I
Fnu4HI FokI HinfI HphI ItaI
MaeIII MboI MboII MnlI MslI
MvaI NcoI NdeII NlaIII NlaIV
PleI RsaI Sau3AI Sau96I ScrFI
StyI TseI Tsp45I