Restriction map for
pFN21AE3077
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# NO CUT ENZYMES ( 166 enzymes) #
##################################
AatII Acc65I AccI AccII AclI
AcyI AfaI AflII AflIII AhdI
Alw44I AlwNI AocI Aor51HI ApaI
ApaLI AscI AseI AsnI Asp700I
Asp718I AspEI AspHI AspI AviII
AvrII BamHI BbeI BbrPI BbsI
BbvCI BcgI BciVI BclI BcnI
BfaI BfrI BglII BlnI BlpI
BpmI Bpu1102I BpuAI BsaAI BsaBI
BsaHI BsaI BsgI BsiEI BsiHKAI
BsiWI BsmBI BsmI Bsp1407I BspDI
BspHI BspLU11I BspMI BsrBI BsrDI
BsrGI BssHII BssSI Bst1107I BstAPI
BstBI BstEII BstPI BstUI BstYI
BstZ17I Bsu36I CelII ClaI CpoI
DraI DrdI DsaI EagI Eam1105I
EarI EclXI Eco47III Eco52I Eco81I
EcoO65I EcoRI EcoRV EcoT14I FauI
FbaI FseI FspI HaeII HgaI
Hin1I HincII HindII HindIII HpaI
KasI KpnI Ksp632I KspI MaeI
MaeII MaeIII MamI MfeI MflI
MluI MspA1I MunI MvnI NaeI
NarI NciI NcoI NdeI NgoMIV
NheI NlaIII NotI NruI NspI
NspV PacI PflFI PmaCI PmeI
PmlI PshAI PshBI Psp1406I PvuI
PvuII RcaI RsaI RsrII SacI
SacII SalI SapI ScaI SexAI
SfiI SfoI SfuI SgrAI SmaI
SnaBI SpeI SphI SspBI SspI
StuI StyI SwaI Tsp45I Tth111I
XbaI XcmI XhoII XmaCI XmaI
XmnI
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# ONE CUT ENZYMES ( 49 enzymes) #
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AccIII 83 TCCGGA T^CCGGA
AcsI 187 AAATTT [GA]^AATT[CT]
AgeI 69 ACCGGT A^CCGGT
AlwI 86 GGATC GGATC(4/5)
ApoI 187 AAATTT [GA]^AATT[CT]
AvaI 43 CTCGAG C^[CT]CG[GA]G
BalI 128 TGGCCA TGG^CCA
BanI 245 GGCACC G^G[CT][GA]CC
BanII 397 GAGCCC G[GA]GC[CT]^C
BbvI 9 GCAGC GCAGC(8/12)
BglI 511 GCCACTAAGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
BmyI 397 GAGCCC G[AGT]GC[ACT]^C
BseAI 83 TCCGGA T^CCGGA
BsmAI 455 GAGAC GTCTC(1/5)
BsmFI 547 GTCCC GGGAC(10/14)
BsoBI 43 CTCGAG C^[CT]CG[GA]G
Bsp1286I 397 GAGCCC G[AGT]GC[ACT]^C
BspEI 83 TCCGGA T^CCGGA
BsrFI 69 ACCGGT [GA]^CCGG[CT]
BsrI 119 ACTGG ACTGG(1/-1)
BstXI 551 CCACAACCCTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
Cac8I 23 GCTCGC GC[ACGT]^[ACGT]GC
CfoI 178 GCGC GCG^C
Cfr10I 69 ACCGGT [GA]^CCGG[CT]
DpnII 87 GATC ^GATC
DraIII 311 CACAGAGTG CAC[ACGT][ACGT][ACGT]^GTG
EaeI 128 TGGCCA [CT]^GGCC[GA]
EcoNI 57 CCTGCAGGAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
EcoT22I 276 ATGCAT ATGCA^T
HhaI 178 GCGC GCG^C
HinP1I 178 GCGC G^CGC
MboI 87 GATC ^GATC
MluNI 128 TGGCCA TGG^CCA
MroI 83 TCCGGA T^CCGGA
MscI 128 TGGCCA TGG^CCA
MslI 90 CACCCTGGTG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
NdeII 87 GATC ^GATC
NsiI 276 ATGCAT ATGCA^T
PaeR7I 43 CTCGAG C^TCGAG
PinAI 69 ACCGGT A^CCGGT
PpuMI 545 AGGTCCC [GA]G^G[AT]CC[CT]
PstI 58 CTGCAG CTGCA^G
Sau3AI 87 GATC ^GATC
SbfI 57 CCTGCAGG CCTGCA^GG
SmlI 43 CTCGAG C^T[CT][GA]AG
Sse8387I 57 CCTGCAGG CCTGCA^GG
TseI 9 GCAGC G^C[AT]GC
TspRI 386 CACTG CA[GC]TG[ACGT][ACGT]^
XhoI 43 CTCGAG C^TCGAG
###################################
# TWO CUT ENZYMES ( 24 enzymes) #
###################################
AvaII 139 GGACC G^G[AT]CC
546 GGTCC
BsaJI 92 CCCTGG C^C[ACGT][ACGT]GG
557 CCCTGG
BsaWI 69 ACCGGT [AT]^CCGG[AT]
83 TCCGGA
BssKI 93 CCTGG ^CC[ACGT]GG
558 CCTGG
BstF5I 292 CATCC GGATG(2/0)
534 GGATG
BstNI 93 CCTGG CC^[AT]GG
558 CCTGG
DraII 32 AGGCCCT [GA]G^G[ACGT]CC[CT]
545 AGGTCCC
EcoO109I 32 AGGCCCT [GA]G^G[ACGT]CC[CT]
545 AGGTCCC
EcoRII 93 CCTGG ^CC[AT]GG
558 CCTGG
Fnu4HI 9 GCAGC GC^[ACGT]GC
164 GCGGC
FokI 292 CATCC GGATG(9/13)
534 GGATG
HapII 70 CCGG C^CGG
84 CCGG
HpaII 70 CCGG C^CGG
84 CCGG
HphI 89 TCACC GGTGA(8/7)
211 TCACC
ItaI 9 GCAGC GC^[ACGT]GC
164 GCGGC
MspI 70 CCGG C^CGG
84 CCGG
MvaI 93 CCTGG CC^[AT]GG
558 CCTGG
MwoI 390 GCTTAATGAGC GC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GC
511 GCCACTAAGGC
PflMI 236 CCAAAATGTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
310 CCACAGAGTGG
PleI 103 GAGTC GAGTC(4/5)
709 GAGTC
ScrFI 93 CCTGG CC^[ACGT]GG
558 CCTGG
SfcI 58 CTGCAG C^T[GA][CT]AG
299 CTGTAG
Tsp509I 188 AATT ^AATT
489 AATT
Van91I 236 CCAAAATGTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
310 CCACAGAGTGG
#########################################
# 3 OR MORE CUT ENZYMES ( 19 enzymes) #
#########################################
AciI AluI BseRI BsiYI BslI
Cfr13I DdeI HaeIII HinfI MboII
MnlI MseI NlaIV Sau96I SfaNI
TaqI TfiI Tru9I TthHB8I