Restriction map for
pFN21AE6664
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# NO CUT ENZYMES ( 151 enzymes) #
##################################
AatII Acc65I AccI AccII AccIII
AclI AcyI AflII AgeI AhdI
Alw44I AlwNI AocI Aor51HI ApaI
ApaLI AscI AseI AsnI Asp718I
AspEI AviII AvrII BalI BamHI
BanII BbeI BbrPI BbsI BbvCI
BcgI BfrI BglI BglII BlnI
BpmI BpuAI BsaAI BsaBI BsaHI
BseAI BsgI BsiEI BsiWI BsmBI
BsmI BspDI BspEI BspHI BspMI
BsrBI BsrDI BsrFI BssHII Bst1107I
BstAPI BstBI BstEII BstPI BstUI
BstXI BstYI BstZ17I Bsu36I CfoI
Cfr10I ClaI CpoI DraI DraIII
DrdI EaeI EagI Eam1105I EclXI
Eco47III Eco52I Eco81I EcoO65I EcoRI
EcoRV EcoT22I FauI FseI FspI
HaeII HgaI HhaI Hin1I HinP1I
HpaI HphI KasI KpnI KspI
MaeII MamI MfeI MflI MluI
MluNI MroI MscI MspA1I MunI
MvnI NaeI NarI NdeI NgoMIV
NheI NotI NruI NsiI NspV
PacI PaeR7I PinAI PmaCI PmeI
PmlI PshBI Psp1406I PstI PvuI
PvuII RcaI RsrII SacI SacII
SalI SapI SbfI ScaI SexAI
SfcI SfiI SfoI SfuI SgrAI
SnaBI SpeI SphI Sse8387I SspI
SwaI TspRI XbaI XcmI XhoI
XhoII
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# ONE CUT ENZYMES ( 58 enzymes) #
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AflIII 261 ACATGT A^C[GA][CT]GT
AlwI 64 GATCC GGATC(4/5)
Asp700I 380 GAAGTCATTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
AspHI 676 GAGCAC G[AT]GC[AT]^C
AspI 700 GACCGGGTC GAC[ACGT]^[ACGT][ACGT]GTC
AvaI 536 CCCGGG C^[CT]CG[GA]G
BbvI 189 GCAGC GCAGC(8/12)
BciVI 102 GGATAC GTATCC(6/5)
BclI 330 TGATCA T^GATCA
BfaI 475 CTAG C^TAG
BlpI 421 GCTGAGC GC^T[ACGT]AGC
BmyI 676 GAGCAC G[AGT]GC[ACT]^C
Bpu1102I 421 GCTGAGC GC^T[ACGT]AGC
BsaI 357 GAGACC GGTCTC(1/5)
BsaWI 482 TCCGGT [AT]^CCGG[AT]
BsiHKAI 676 GAGCAC G[AT]GC[AT]^C
BsoBI 536 CCCGGG C^[CT]CG[GA]G
Bsp1286I 676 GAGCAC G[AGT]GC[ACT]^C
Bsp1407I 256 TGTACA T^GTACA
BspLU11I 261 ACATGT A^CATGT
BsrGI 256 TGTACA T^GTACA
BsrI 822 CCAGT ACTGG(1/-1)
BssSI 679 CACGAG CACGAG(-5/-1)
Cac8I 116 GCAAGC GC[ACGT]^[ACGT]GC
CelII 421 GCTGAGC GC^T[ACGT]AGC
DraII 519 GGGACCT [GA]G^G[ACGT]CC[CT]
DsaI 647 CCATGG C^C[GA][CT]GG
EarI 247 GAAGAG CTCTTC(1/4)
EcoNI 766 CCTCAGATAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
EcoO109I 519 GGGACCT [GA]G^G[ACGT]CC[CT]
EcoT14I 647 CCATGG C^C[AT][AT]GG
FbaI 330 TGATCA T^GATCA
Fnu4HI 189 GCAGC GC^[ACGT]GC
HincII 214 GTCAAC GT[CT]^[GA]AC
HindII 214 GTCAAC GT[CT]^[GA]AC
ItaI 189 GCAGC GC^[ACGT]GC
Ksp632I 247 GAAGAG CTCTTC(1/4)
MaeI 475 CTAG C^TAG
NcoI 647 CCATGG C^CATGG
NspI 261 ACATGT [GA]CATG^[CT]
PflFI 700 GACCGGGTC GAC[ACGT]^[ACGT][ACGT]GTC
PflMI 779 CCACAGAATGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
PleI 479 GACTC GAGTC(4/5)
PpuMI 519 GGGACCT [GA]G^G[AT]CC[CT]
SmaI 536 CCCGGG CCC^GGG
SmlI 291 CTTGAG C^T[CT][GA]AG
SspBI 256 TGTACA T^GTACA
StuI 54 AGGCCT AGG^CCT
StyI 647 CCATGG C^C[AT][AT]GG
TaqI 515 TCGA T^CGA
TfiI 303 GATTC G^A[AT]TC
TseI 189 GCAGC G^C[AT]GC
Tth111I 700 GACCGGGTC GAC[ACGT]^[ACGT][ACGT]GTC
TthHB8I 515 TCGA T^CGA
Van91I 779 CCACAGAATGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
XmaCI 536 CCCGGG C^CCGGG
XmaI 536 CCCGGG C^CCGGG
XmnI 380 GAAGTCATTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
###################################
# TWO CUT ENZYMES ( 20 enzymes) #
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AciI 23 CCGC CCGC(-3/-1)
571 GCGG
AcsI 71 AAATTT [GA]^AATT[CT]
626 GAATTT
AfaI 257 GTAC GT^AC
560 GTAC
ApoI 71 AAATTT [GA]^AATT[CT]
626 GAATTT
BanI 10 GGCACC G^G[CT][GA]CC
485 GGTGCC
BseRI 298 GAGGAG GAGGAG(10/8)
398 GAGGAG
BsmAI 357 GAGAC GTCTC(1/5)
460 GAGAC
BsmFI 499 GGGAC GGGAC(10/14)
519 GGGAC
DpnII 64 GATC ^GATC
331 GATC
HindIII 546 AAGCTT A^AGCTT
744 AAGCTT
HinfI 303 GATTC G^A[ACGT]TC
479 GACTC
MboI 64 GATC ^GATC
331 GATC
MseI 133 TTAA T^TAA
709 TTAA
NdeII 64 GATC ^GATC
331 GATC
PshAI 359 GACCTCAGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC
501 GACAATGGTC
RsaI 257 GTAC GT^AC
560 GTAC
Sau3AI 64 GATC ^GATC
331 GATC
SfaNI 127 GATGC GCATC(5/9)
164 GCATC
Tru9I 133 TTAA T^TAA
709 TTAA
Tsp45I 271 GTGAC ^GT[GC]AC
508 GTCAC
#########################################
# 3 OR MORE CUT ENZYMES ( 29 enzymes) #
#########################################
AluI AvaII BcnI BsaJI BsiYI
BslI BssKI BstF5I BstNI Cfr13I
DdeI EcoRII FokI HaeIII HapII
HpaII MaeIII MboII MnlI MslI
MspI MvaI MwoI NciI NlaIII
NlaIV Sau96I ScrFI Tsp509I