Restriction map for
pFN21AE1670
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# NO CUT ENZYMES ( 181 enzymes) #
##################################
AatII Acc65I AccI AccII AccIII
AciI AclI AcyI AfaI AflII
AflIII AgeI AhdI Alw44I AocI
Aor51HI ApaI ApaLI AscI AseI
AsnI Asp718I AspEI AspI AvaI
AviII AvrII BalI BamHI BanI
BanII BbeI BbrPI BbsI BbvCI
BcgI BciVI BclI BcnI BfrI
BglI BlnI BpmI BpuAI BsaAI
BsaBI BsaHI BsaWI BseAI BsgI
BsiEI BsiWI BsmBI BsmFI BsmI
BsoBI Bsp1407I BspDI BspEI BspLU11I
BspMI BsrBI BsrDI BsrFI BsrGI
BssHII BssSI Bst1107I BstAPI BstBI
BstEII BstPI BstUI BstXI BstZ17I
Bsu36I Cac8I CfoI Cfr10I ClaI
CpoI DraI DraIII DrdI DsaI
EaeI EagI Eam1105I EclXI Eco47III
Eco52I Eco81I EcoO65I EcoRI EcoRV
EcoT14I EcoT22I FauI FbaI FseI
FspI HaeII HapII HgaI HhaI
Hin1I HinP1I HindIII HpaI HpaII
KasI KpnI KspI MaeII MamI
MfeI MluI MluNI MroI MscI
MslI MspI MunI MvnI NaeI
NarI NciI NcoI NdeI NgoMIV
NheI NlaIV NotI NruI NsiI
NspI NspV PacI PaeR7I PflFI
PinAI PmaCI PmeI PmlI PshAI
PshBI Psp1406I PstI PvuI RsaI
RsrII SacI SacII SalI SapI
SbfI ScaI SexAI SfcI SfiI
SfoI SfuI SgrAI SmaI SmlI
SnaBI SpeI SphI Sse8387I SspBI
SspI StuI StyI SwaI TaqI
Tth111I TthHB8I XbaI XhoI XmaCI
XmaI
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# ONE CUT ENZYMES ( 39 enzymes) #
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AcsI 316 AAATTC [GA]^AATT[CT]
AlwI 246 GATCC GGATC(4/5)
AlwNI 337 CAGCAGCTG CAG[ACGT][ACGT][ACGT]^CTG
ApoI 316 AAATTC [GA]^AATT[CT]
Asp700I 429 GAAATACTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
AspHI 37 GTGCTC G[AT]GC[AT]^C
AvaII 107 GGACC G^G[AT]CC
BfaI 310 CTAG C^TAG
BlpI 39 GCTCAGC GC^T[ACGT]AGC
BmyI 37 GTGCTC G[AGT]GC[ACT]^C
Bpu1102I 39 GCTCAGC GC^T[ACGT]AGC
BsaI 301 GAGACC GGTCTC(1/5)
BsiHKAI 37 GTGCTC G[AT]GC[AT]^C
Bsp1286I 37 GTGCTC G[AGT]GC[ACT]^C
BspHI 509 TCATGA T^CATGA
CelII 39 GCTCAGC GC^T[ACGT]AGC
DdeI 40 CTCAG C^T[ACGT]AG
DraII 106 AGGACCT [GA]G^G[ACGT]CC[CT]
EarI 265 CTCTTC CTCTTC(1/4)
EcoNI 396 CCTGATGAAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
EcoO109I 106 AGGACCT [GA]G^G[ACGT]CC[CT]
HincII 528 GTCAAC GT[CT]^[GA]AC
HindII 528 GTCAAC GT[CT]^[GA]AC
HphI 381 GGTGA GGTGA(8/7)
Ksp632I 265 CTCTTC CTCTTC(1/4)
MaeI 310 CTAG C^TAG
MaeIII 382 GTGAC ^GT[ACGT]AC
MseI 550 TTAA T^TAA
PflMI 87 CCACAGCCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
PpuMI 106 AGGACCT [GA]G^G[AT]CC[CT]
RcaI 509 TCATGA T^CATGA
SfaNI 114 GATGC GCATC(5/9)
Tru9I 550 TTAA T^TAA
Tsp45I 382 GTGAC ^GT[GC]AC
Tsp509I 317 AATT ^AATT
TspRI 324 CACTG CA[GC]TG[ACGT][ACGT]^
Van91I 87 CCACAGCCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
XcmI 186 CCAGGAGGAGTTTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
XmnI 429 GAAATACTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
###################################
# TWO CUT ENZYMES ( 17 enzymes) #
###################################
BglII 254 AGATCT A^GATCT
514 AGATCT
BseRI 132 GAGGAG GAGGAG(10/8)
190 GAGGAG
BsiYI 87 CCACAGCCTGG CC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GG
396 CCTGATGAAGG
BslI 87 CCACAGCCTGG CC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GG
396 CCTGATGAAGG
BsmAI 301 GAGAC GTCTC(1/5)
388 GAGAC
BsrI 21 ACTGG ACTGG(1/-1)
204 CCAGT
BstF5I 225 CATCC GGATG(2/0)
297 GGATG
BstYI 254 AGATCT [GA]^GATC[CT]
514 AGATCT
Cfr13I 27 GGCCC G^G[ACGT]CC
107 GGACC
FokI 225 CATCC GGATG(9/13)
297 GGATG
HaeIII 3 GGCC GG^CC
27 GGCC
MflI 254 AGATCT [GA]^GATC[CT]
514 AGATCT
MspA1I 42 CAGCTG C[AC]G^C[GT]G
340 CAGCTG
PvuII 42 CAGCTG CAG^CTG
340 CAGCTG
Sau96I 27 GGCCC G^G[ACGT]CC
107 GGACC
TfiI 137 GAATC G^A[AT]TC
443 GAATC
XhoII 254 AGATCT [GA]^GATC[CT]
514 AGATCT
#########################################
# 3 OR MORE CUT ENZYMES ( 21 enzymes) #
#########################################
AluI BbvI BsaJI BssKI BstNI
DpnII EcoRII Fnu4HI HinfI ItaI
MboI MboII MnlI MvaI MwoI
NdeII NlaIII PleI Sau3AI ScrFI
TseI