Restriction map for
pFN21AE1672
##################################
# NO CUT ENZYMES ( 191 enzymes) #
##################################
AatII Acc65I AccI AccII AccIII
AciI AclI AcsI AcyI AflII
AflIII AgeI AhdI Alw44I AocI
Aor51HI ApaI ApaLI ApoI AscI
AseI AsnI Asp700I Asp718I AspEI
AspI AvaI AviII AvrII BalI
BamHI BanI BanII BbeI BbrPI
BbsI BbvCI BcgI BciVI BclI
BcnI BfrI BglI BglII BlnI
BpmI BpuAI BsaAI BsaBI BsaHI
BsaI BsaWI BseAI BsgI BsiEI
BsiWI BsmAI BsmBI BsmI BsoBI
Bsp1407I BspDI BspEI BspLU11I BspMI
BsrBI BsrDI BsrFI BsrGI BssHII
BssSI Bst1107I BstAPI BstBI BstEII
BstPI BstUI BstXI BstZ17I Bsu36I
Cac8I CfoI Cfr10I ClaI CpoI
DraI DraII DraIII DrdI DsaI
EaeI EagI Eam1105I EclXI Eco47III
Eco52I Eco81I EcoO109I EcoO65I EcoRI
EcoRV EcoT14I EcoT22I FauI FbaI
FseI FspI HaeII HapII HgaI
HhaI Hin1I HinP1I HincII HindII
HindIII HpaI HpaII KasI KpnI
KspI MaeII MamI MfeI MluI
MluNI MroI MscI MslI MspI
MunI MvnI NaeI NarI NciI
NcoI NdeI NgoMIV NheI NlaIV
NotI NruI NsiI NspI NspV
PacI PaeR7I PflFI PinAI PmaCI
PmeI PmlI PpuMI PshAI PshBI
Psp1406I PstI PvuI RsrII SacI
SacII SalI SapI SbfI ScaI
SexAI SfcI SfiI SfoI SfuI
SgrAI SmaI SmlI SnaBI SpeI
SphI Sse8387I SspBI SspI StuI
StyI SwaI TaqI Tsp509I TspRI
Tth111I TthHB8I XhoI XmaCI XmaI
XmnI
###################################
# ONE CUT ENZYMES ( 33 enzymes) #
###################################
AfaI 474 GTAC GT^AC
AlwI 518 GATCC GGATC(4/5)
AlwNI 340 CAGCAGCTG CAG[ACGT][ACGT][ACGT]^CTG
AspHI 37 GTGCTC G[AT]GC[AT]^C
AvaII 107 GGACC G^G[AT]CC
BfaI 313 CTAG C^TAG
BlpI 39 GCTCAGC GC^T[ACGT]AGC
BmyI 37 GTGCTC G[AGT]GC[ACT]^C
Bpu1102I 39 GCTCAGC GC^T[ACGT]AGC
BsiHKAI 37 GTGCTC G[AT]GC[AT]^C
BsmFI 391 GGGAC GGGAC(10/14)
Bsp1286I 37 GTGCTC G[AGT]GC[ACT]^C
BspHI 512 TCATGA T^CATGA
BsrI 207 CCAGT ACTGG(1/-1)
BstYI 517 AGATCC [GA]^GATC[CT]
CelII 39 GCTCAGC GC^T[ACGT]AGC
EcoNI 237 CCTCCATGAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
HaeIII 27 GGCC GG^CC
HphI 246 GGTGA GGTGA(8/7)
MaeI 313 CTAG C^TAG
MaeIII 98 GTCAC ^GT[ACGT]AC
MflI 517 AGATCC [GA]^GATC[CT]
MseI 553 TTAA T^TAA
MwoI 47 GCAAGTCAAGC GC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GC
PflMI 87 CCACAGCCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
RcaI 512 TCATGA T^CATGA
RsaI 474 GTAC GT^AC
Tru9I 553 TTAA T^TAA
Tsp45I 98 GTCAC ^GT[GC]AC
Van91I 87 CCACAGCCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
XbaI 312 TCTAGA T^CTAGA
XcmI 110 CCATGATGCTCCTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
XhoII 517 AGATCC [GA]^GATC[CT]
###################################
# TWO CUT ENZYMES ( 18 enzymes) #
###################################
BseRI 132 GAGGAG GAGGAG(10/8)
190 GAGGAG
BsiYI 87 CCACAGCCTGG CC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GG
237 CCTCCATGAGG
BslI 87 CCACAGCCTGG CC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GG
237 CCTCCATGAGG
BstF5I 417 CATCC GGATG(2/0)
300 GGATG
Cfr13I 27 GGCCC G^G[ACGT]CC
107 GGACC
DdeI 40 CTCAG C^T[ACGT]AG
80 CTCAG
DpnII 73 GATC ^GATC
518 GATC
EarI 268 CTCTTC CTCTTC(1/4)
525 CTCTTC
FokI 417 CATCC GGATG(9/13)
300 GGATG
Ksp632I 268 CTCTTC CTCTTC(1/4)
525 CTCTTC
MboI 73 GATC ^GATC
518 GATC
MboII 269 TCTTC GAAGA(8/7)
526 TCTTC
MspA1I 42 CAGCTG C[AC]G^C[GT]G
343 CAGCTG
NdeII 73 GATC ^GATC
518 GATC
PvuII 42 CAGCTG CAG^CTG
343 CAGCTG
Sau3AI 73 GATC ^GATC
518 GATC
Sau96I 27 GGCCC G^G[ACGT]CC
107 GGACC
SfaNI 114 GATGC GCATC(5/9)
369 GATGC
#########################################
# 3 OR MORE CUT ENZYMES ( 16 enzymes) #
#########################################
AluI BbvI BsaJI BssKI BstNI
EcoRII Fnu4HI HinfI ItaI MnlI
MvaI NlaIII PleI ScrFI TfiI
TseI